KLHL23 (PHOSPHO2-KLHL23) (NM_001199290) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232968 representing NM_001199290 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTAAAAGGACAAGAAGATTATATTTATCTTTTCAAGGATTCAACACATCCAGTGGATTTTCTGG ATGCATTCAGAACATTTTACTTGGATGGATTATTTACTGATATTACTCTTCAGTGTCCTTCAGGCATAAT TTTCCATTGTCACCGAGCCGTTTTAGCTGCTTGCAGCAATTATTTTAAGGCAATGTTCACAGCTGACATG AAAGAAAAATTTAAAAATAAAATAAAACTCTCTGGCATCCACCATGATATTCTGGAAGGCCTTGTAAATT ATGCATACACTTCCCAAATTGAAATAACTAAAAGAAATGTTCAAAGCCTGCTTGAGGCAGCGGATCTGCT ACAGTTCCTTTCAGTAAAGAAGGCTTGTGAGCGGTTTTTGGTAAGGCACTTGGATATTGATAATTGTATT GGAATGCACTCCTTTGCAGAATTTCATGTGTGTCCAGAACTAGAGAAGGAATCTCGAAGAATTCTATGTT CAAAGTTTAAGGAAGTGTGGCAACAAGAAGAATTTCTGGAAATCAGCCTTGAAAAGTTTCTCTTTATCTT GTCCAGAAAGAATCTCAGTGTTTGGAAAGAAGAAGCTATCATAGAGCCAGTTATTAAGTGGACTGCTCAT GATGTAGAAAATCGAATTGAATGCCTCTATAATCTACTGAGCTATATCAACATTGATATAGATCCAGTGT ACTTAAAAACAGCCTTAGGCCTTCAAAGAAGCTGCCTGCTCACCGAAAATAAGATCCGCTCCCTAATATA CAATGCCTTGAATCCCATGCATAAAGAGATTTCCCAGAGGTCCACAGCCACAATGTATATAATTGGAGGC TATTACTGGCATCCTTTATCAGAGGTTCACATATGGGATCCTTTGACAAATGTTTGGATTCAGGGAGCAG AAATACCAGATTATACCAGGGAGAGCTATGGTGTTACATGTTTAGGACCCAACATTTATGTAACTGGGGG CTACAGGACGGATAACATAGAAGCTCTTGACACAGTGTGGATCTATAACAGTGAAAGTGATGAATGGACA GAAGGTTTGCCAATGCTCAATGCCAGGTATTACCACTGTGCAGTCACCTTGGGTGGCTGTGTCTATGCTT TAGGTGGTTACAGAAAAGGGGCTCCAGCAGAAGAGGCTGAGTTCTATGATCCTTTAAAAGAGAAATGGAT TCCTATTGCAAACATGATTAAAGGTGTGGGAAATGCTACTGCCTGTGTCTTACATGATGTTATCTACGTC ATTGGTGGCCACTGTGGCTACAGAGGAAGCTGCACCTATGACAAAGTTCAGAGCTACAATTCCGATATCA ACGAATGGAGCCTCATCACCTCCAGTCCACATCCAGAATATGGATTGTGCTCAGTTCCGTTTGAAAATAA GCTCTATCTAGTCGGTGGACAAACTACAATCACAGAATGCTATGACCCTGAACAAAATGAATGGAGAGAG ATAGCTCCCATGATGGAAAGGAGGATGGAGTGCGGTGCCGTCATCATGAATGGATGTATTTATGTCACTG GAGGATACTCCTACTCAAAGGGAACGTATCTTCAGAGCATTGAGAAATATGATCCAGATCTTAATAAGTG GGAAATAGTGGGTAATCTTCCCAGTGCCATGCGGTCTCATGGGTGTGTTTGTGTGTATAATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232968 representing NM_001199290 Red=Cloning site Green=Tags(s) MALKGQEDYIYLFKDSTHPVDFLDAFRTFYLDGLFTDITLQCPSGIIFHCHRAVLAACSNYFKAMFTADM KEKFKNKIKLSGIHHDILEGLVNYAYTSQIEITKRNVQSLLEAADLLQFLSVKKACERFLVRHLDIDNCI GMHSFAEFHVCPELEKESRRILCSKFKEVWQQEEFLEISLEKFLFILSRKNLSVWKEEAIIEPVIKWTAH DVENRIECLYNLLSYINIDIDPVYLKTALGLQRSCLLTENKIRSLIYNALNPMHKEISQRSTATMYIIGG YYWHPLSEVHIWDPLTNVWIQGAEIPDYTRESYGVTCLGPNIYVTGGYRTDNIEALDTVWIYNSESDEWT EGLPMLNARYYHCAVTLGGCVYALGGYRKGAPAEEAEFYDPLKEKWIPIANMIKGVGNATACVLHDVIYV IGGHCGYRGSCTYDKVQSYNSDINEWSLITSSPHPEYGLCSVPFENKLYLVGGQTTITECYDPEQNEWRE IAPMMERRMECGAVIMNGCIYVTGGYSYSKGTYLQSIEKYDPDLNKWEIVGNLPSAMRSHGCVCVYNV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199290
ORF Size	1674 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199290.3
RefSeq Size	4206 bp
RefSeq ORF	1677 bp
Locus ID	100526832
UniProt ID	Q8NBE8
MW	63.9 kDa
Gene Summary	This locus represents naturally occurring read-through transcription between the neighboring PHOSPHO2 (phosphatase, orphan 2) and KLHL23 (kelch-like 23) genes on chromosome 2. The read-through transcript includes only non-coding PHOSPHO2 exons, and thus encodes the KLHL23 protein. [provided by RefSeq, Dec 2010]

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