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ROBO1 (NM_001145844) Human Tagged ORF Clone
CAT＃: RC227713
ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3
ORF Plasmid: tGFP
"NM_001145844" in other vectors (6)
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CNY 16,480.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | axon guidance receptor; DUTT1; FLJ21882; MGC131599; MGC133277; roundabout, axon guidance receptor, homolog 1 (Drosophila); roundabout 1; SAX3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227713 representing NM_001145844
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCTTCGTC AGGAAGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTCTCAAAAGGAGAACCTGCAAC TTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATTGAATGGTACAAAGGGGGAGAGAGAGTGGAG ACAGACAAAGATGACCCTCGCTCACACCGAATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAG TACATGGACGGAAAAGTAGACCTGATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGC TGTGAGCCACAATGCATCGCTGGAAGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTC ATGGTTGCAGTAGGAGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCACCATTT CATGGAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACTATACGAGGAGGAAAGCTCAT GATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTTTGTGTTGGTACCAATATGGTTGGGGAACGT GAGAGTGAAGTAGCCGAGCTGACTGTCTTAGAGAGACCATCATTTGTGAAGAGACCCAGTAACTTGGCAG TAACTGTGGATGACAGTGCAGAATTTAAATGTGAGGCCCGAGGTGACCCTGTACCTACAGTACGATGGAG GAAAGATGATGGAGAGCTGCCCAAATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAG GTGACAGCTGGTGACATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGCATCTG CTACTCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAACCCCGTGACCAGGTTGTTGC TTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGAAATCCTCAACCAGCTATTTTCTGGAGGAGA GAAGGGAGTCAGAATCTACTTTTCTCATATCAACCACCACAGTCATCCAGCCGATTTTCAGTCTCCCAGA CTGGCGACCTCACAATTACTAATGTCCAGCGATCTGATGTTGGTTATTACATCTGCCAGACTTTAAATGT TGCTGGAAGCATCATCACAAAGGCATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTT ATTCGACAAGGTCCTGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGTGTGGCCACAG GCAGTCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCAACCCAAGACTCTCGAATCAA ACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAGCTGGGTGATACTGGTCGGTACACCTGCATT GCATCAACCCCCAGTGGTGAAGCAACATGGAGTGCTTACATTGAAGTTCAAGAATTTGGAGTTCCAGTTC AGCCTCCAAGACCTACTGACCCAAATTTAATCCCTAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAG CAGAAATACAGTCACATTATCATGGCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATA GAAGCCTTCAGCCATGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACAGAAACATCTG CCATTAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCAGCTAATGCATATGGAATTAG TGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGATGTCCTACCAACAAGTCAGGGGGTGGACCAC AAGCAGGTCCAGAGAGAGCTGGGAAATGCTGTTCTGCACCTCCACAACCCCACCGTCCTTTCTTCCTCTT CCATCGAAGTGCACTGGACAGTAGATCAACAGTCTCAGTATATACAAGGATATAAAATTCTCTATCGGCC ATCTGGAGCCAACCACGGAGAATCAGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTG GTAATCCCTGATCTCAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAATGAATTTCAAG GAGCAGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGTGCCCCACCCCAAGGTGTAAC TGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTTAGTTGGCAGCCACCTCCAGAAGACACTCAA AATGGAATGGTCCAAGAGTATAAGGTTTGGTGTCTGGGCAATGAAACTCGATACCACATCAACAAAACAG TGGATGGTTCCACCTTTTCCGTGGTCATTCCCTTTCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGC AGCCAGCACTGGGGCTGGGTCTGGGGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAAC CCTGTGTCACCTGAGGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCA TAGCAGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATCTGGCTTTATCGACACCGCAA GAAGAGAAACGGACTTACTAGTACATACGCGGGTATCAGAAAAGTAACTTACCAGAGAGGAGGCGAAGCT GTCAGCAGTGGAGGGAGGCCTGGACTTCTCAACATCAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACA CGTGGCCTAATACTGGCAACAACCACAATGACTGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAACAG CGACAGCAACCTCACTACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAACCAACTGGATAAC AAACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAACAAAATCAATG AGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTTTGTCAATCCATCAGGGCAGCCTACTCC TTACGCCACCACTCAGCTCATCCAGTCAAACCTCAGCAACAACATGAACAATGGCAGCGGGGACTCTGGC GAGAAGCACTGGAAACCACTGGGACAGCAGAAACAAGAAGTGGCACCAGTTCAGTACAACATCGTGGAGC AAAACAAGCTGAACAAAGATTATCGAGCAAATGACACAGTTCCTCCAACTATCCCATACAACCAATCATA CGACCAGAACACAGGAGGATCCTACAACAGCTCAGACCGGGGCAGTAGTACATCTGGGAGTCAGGGGCAC AAGAAAGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCATGAACTGGGCAGACCTGCTTCCTCCTC CCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGAGTACAACATTTCTGTAGATGAAAGCTATGACCA AGAAATGCCATGTCCCGTGCCACCAGCAAGGATGTATTTGCAACAAGATGAATTAGAAGAGGAGGAAGAT GAACGAGGCCCCACTCCCCCTGTTCGGGGAGCAGCTTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGT CCACTGCCACTCTGACTCCCTCCCCACAGGAAGAACTCCAGCCCATGTTACAGGATTGTCCAGAGGAGAC TGGCCACATGCAGCACCAGCCCGACAGGAGACGGCAGCCTGTGAGTCCTCCTCCACCACCACGGCCGATC TCCCCTCCACATACCTATGGCTACATTTCAGGACCCCTGGTCTCAGATATGGATACGGATGCGCCAGAAG AGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGATGCAAACCAGAAGGCTTTTGTTACGTGGGCTTGA GCAGACACCTGCCTCCAGTGTTGGGGACCTGGAGAGCTCTGTCACGGGGTCCATGATCAACGGCTGGGGC TCAGCCTCAGAGGAGGACAACATTTCCAGCGGACGCTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCA CTGATGCTGACTTTGCCCAGGCAGTCGCAGCAGCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACGGCA AATGCAGGATGCTGCTGGCCGTCGACATTTTCATGCGTCTCAGTGCCCTAGGCCCACAAGTCCCGTGTCT ACAGACAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGACCAGCCAAGAAACTGAAACACCAGCCAG GACATCTGCGCAGAGAAACCTACACAGATGATCTTCCACCACCTCCTGTGCCGCCACCTGCTATAAAGTC ACCTACTGCCCAATCCAAGACACAGCTGGAAGTACGACCTGTAGTGGTGCCAAAACTCCCTTCTATGGAT GCAAGAACAGACAGATCATCAGACAGAAAAGGAAGCAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGAC AGGTTGTTGACATGCGAACAAATCCAGGTGATCCCAGAGAAGCACAGGAACAGCAAAATGACGGGAAAGG ACGTGGAAACAAGGCAGCAAAACGAGACCTTCCACCAGCAAAGACTCATCTCATCCAAGAGGATATTCTA CCTTATTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAGATCCCAGTTCCTCAAGCTCAATGTCAT CAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAATGTAGGTCGAAGAAATATTGCAGAAATGCAGGT ACTTGGAGGATATGAAAGAGGAGAAGATAATAATGAAGAATTAGAGGAAACTGAAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227713 representing NM_001145844
Red=Cloning site Green=Tags(s) MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVE TDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDV MVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGER ESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRK VTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRR EGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPV IRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCI ASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYII EAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDH KQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSV VIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQ NGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGN PVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEA VSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDN KQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSG EKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGH KKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEED ERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPI SPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWG SASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVS TDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMD ARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDIL PYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145844 |
| ORF Size | 4818 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145844.1, NP_001139316.1 |
| RefSeq ORF | 4820 bp |
| Locus ID | 6091 |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance |
| MW | 175.7 kDa |
| Gene Summary | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227713L1 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, Myc-DDK-tagged |
CNY 18,880.00 |
|
| RC227713L2 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, mGFP tagged |
CNY 20,210.00 |
|
| RC227713L3 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, Myc-DDK-tagged |
CNY 18,880.00 |
|
| RC227713L4 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, mGFP tagged |
CNY 18,880.00 |
|
| RG227713 | ROBO1 (tGFP-tagged) - Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3 |
CNY 20,140.00 |
|
| SC326515 | ROBO1 (untagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 3, mRNA |
CNY 16,488.00 |
