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Apc5 (ANAPC5) (NM_001137559) Human Tagged ORF Clone
CAT＃: RC226883
- TrueORF®
ANAPC5 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2
ORF Plasmid: tGFP
"NM_001137559" in other vectors (4)
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CNY 4,784.00
CNY 5,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | APC5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226883 representing NM_001137559
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAGGCGAGTTGAAGGATATGGAACAGTTTTTTGATGACCTTTCAGATTCTTTCTCTGGAACTG AACCAGAGGTTCACAAAACAAGTGTAGTAGGTTTGTTTCTGCGTCACATGATCTTGGCCTACAGTAAGCT TTCTTTCAGCCAAGTGTTTAAACTGTACACTGCCCTTCAGCAGTACTTCCAGAATGGTGAGAAAAAGACA GTGGAGGATGCTGATATGGAACTGACCAGTAGAGATGAGGGTGAAAGAAAAATGGAAAAAGAAGAACTTG ATGTATCTGTAAGAGAAGAGGAGGTATCTTGCAGTGGGCCTCTGTCCCAAAAACAAGCAGAATTTTTTCT TTCTCAACAGGCTTCTTTGCTAAAGAATGATGAGACTAAGGCCCTCACTCCAGCTTCCTTGCAGAAGGAA TTAAACAATTTGTTGAAATTTAATCCTGATTTTGCTGAAGCGCATTATCTCAGCTACTTAAACAACCTCC GTGTCCAAGATGTTTTCAGTTCAACACACAGTCTCCTCCATTATTTTGATCGTCTGATTCTTACCGGAGC CGAAAGCAAAAGTAATGGGGAAGAGGGCTATGGCCGGAGCTTGAGATACGCCGCTCTGAATCTTGCCGCC CTGCACTGCCGCTTCGGTCACTATCAACAGGCAGAGCTCGCCCTGCAGGAGGCAATTAGGATTGCCCAGG AGTCCAACGATCACGTGTGTCTCCAGCACTGTTTGAGCTGGCTTTATGTGCTGGGGCAGAAGAGATCCGA TAGCTATGTTCTGCTGGAGCATTCTGTGAAGAAGGCAGTACATTTTGGGTTACCGAGAGCTTTTGCTGGG AAGACGGCAAACAAGCTGATGGATGCCCTAAAGGACTCCGACCTCCTGCACTGGAAACACAGCCTGTCAG AGCTCATCGATATCAGCATCGCACAGAAAACGGCCATCTGGAGGCTGTATGGCCGCAGCACCATGGCACT GCAACAGGCCCAGATGTTGCTGAGCATGAACAGCCTGGAGGCGGTGAATGCGGGCGTGCAGCAGAACAAC ACAGAGTCCTTTGCTGTCGCACTCTGCCACCTCGCAGAGCTACACGCGGAGCAGGGCTGTTTTGCTGCAG CTTCTGAAGTGTTAAAGCACTTGAAGGAACGATTTCCGCCTAATAGTCAGCACGCCCAGTTATGGATGCT ATGTGATCAAAAAATACAGTTTGACAGAGCAATGAATGATGGCAAATATCATTTGGCTGATTCACTTGTT ACAGGAATCACAGCTCTCAATAGCATAGAGGGTGTTTATAGGAAAGCGGTTGTATTACAAGCTCAGAACC AAATGTCAGAGGCACATAAGCTTTTACAAAAATTGTTGGTTCATTGTCAGAAACTGAAGAACACAGAAAT GGTGATCAGTGTCCTACTGTCCGTGGCAGAGCTGTACTGGCGATCTTCCTCCCCTACCATCGCGCTGCCC ATGCTCCTGCAGGCTCTGGCCCTCTCCAAGGAGTACCGGTTACAGTACTTGGCCTCTGAAACAGTGCTGA ACTTGGCTTTTGCGCAGCTCATTCTTGGAATCCCAGAACAGGCCTTAAGTCTTCTCCACATGGCCATCGA GCCCATCTTGGCTGACGGGGCTATCCTGGACAAAGGTCGTGCCATGTTCTTAGTGGCCAAGTGCCAGGTG GCTTCAGCAGCTTCCTACGATCAGCCGAAGAAAGCAGAAGCTCTGGAGGCTGCCATCGAGAACCTCAATG AAGCCAAGAACTATTTTGCAAAGGTTGACTGCAAAGAGCGCATCAGGGACGTCGTTTACTTCCAGGCCAG ACTCTACCATACCCTGGGGAAGACCCAGGAGAGGAACCGGTGTGCGATGCTCTTCCGGCAGCTGCATCAG GAGCTGCCCTCTCATGGGGTACCCTTGATAAACCATCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226883 representing NM_001137559
Red=Cloning site Green=Tags(s) MAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQNGEKKT VEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKE LNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAA LHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRSDSYVLLEHSVKKAVHFGLPRAFAG KTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEAVNAGVQQNN TESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLV TGITALNSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALP MLLQALALSKEYRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPILADGAILDKGRAMFLVAKCQV ASAASYDQPKKAEALEAAIENLNEAKNYFAKVDCKERIRDVVYFQARLYHTLGKTQERNRCAMLFRQLHQ ELPSHGVPLINHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001137559 |
| ORF Size | 1929 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001137559.1, NP_001131031.1 |
| RefSeq ORF | 1932 bp |
| Locus ID | 51433 |
| UniProt ID | Q9UJX4 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
| MW | 72.3 kDa |
| Gene Summary | This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226883L3 | Lenti-ORF clone of ANAPC5 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2 |
CNY 7,600.00 |
|
| RC226883L4 | Lenti-ORF clone of ANAPC5 (mGFP-tagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2 |
CNY 7,600.00 |
|
| RG226883 | ANAPC5 (tGFP-tagged) - Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2 |
CNY 6,270.00 |
|
| SC326144 | ANAPC5 (untagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2 |
CNY 10,360.00 |
