DC SIGN (CD209) (NM_001144896) Human Tagged ORF Clone

CAT#: RC226695

  • TrueORF®

CD209 (Myc-DDK-tagged)-Human CD209 molecule (CD209), transcript variant 3. Note: ORF is codon optimized

ORF Plasmid: DDK tGFP



  "NM_001144896" in other vectors (4)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CD209 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1; hDC-SIGN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC226695 ORF sequence, codon optimized.
Due to the complexity of NM_001144896, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical
.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGACAGCAAAGAGCCTAGGCTGCAGCAACTCGGTCTTCTCGAAGAAGAGCAGCTTAGGGGCCTGG
GTTTTCGACAGACTCGGGGCTATAAAAGTCTTGCGGTGAGCAAGGTCCCTTCCTCAATCTCACAGGAACA
GAGCAGACAGGACGCTATTTATCAGAATCTGACACAGTTGAAGGCAGCTGTAGGGGAACTGTCTGAAAAA
AGTAAGTTGCAGGAGATATATCAAGAGCTGACCCAACTGAAAGCAGCTGTGGGGGAGCTGCCTGAGAAAT
CTAAGCTGCAAGAAATCTATCAGGAACTGACACGGCTGAAGGCTGCCGTAGGCGAGCTCCCAGAAAAGAG
CAAGCTGCAAGAAATTTACCAGGAACTCACATGGCTCAAGGCCGCCGTGGGGGAACTCCCCGAAAAATCA
AAGATGCAAGAGATCTACCAGGAACTGACGCGGCTGAAAGCGGCAGTCGGCGAGCTCCCTGAGAAAAGTA
AACAACAGGAGATCTACCAGGAGCTTACACGCCTTAAGGCCGCAGTCGGGGAGCTTCCTGAAAAGTCCAA
GCAGCAGGAGATCTACCAAGAACTGACCAGACTCAAGGCCGCAGTGGGTGAATTGCCGGAGAAGTCTAAG
CAACAGGAGATCTATCAGGAGCTCACCCAATTGAAGGCGGCAGTAGAGAGGTTGTGCCACCCGTGTCCAT
GGGAGTGGACTTTCTTCCAGGGCAACTGTTACTTCATGAGTAATTCACAGCGCAACTGGCATGACAGCAT
CACAGCGTGCAAAGAAGTTGGCGCTCAGCTGGTGGTCATTAAGAGTGCTGAGGAACAGAACTTTCTTCAA
CTGCAGAGCAGCCGATCAAATAGGTTCACTTGGATGGGGCTCAGCGACCTTAACCAGGAAGGAACATGGC
AGTGGGTAGATGGAAGTCCACTGCTCCCGTCATTCAAACAATATTGGAACAGGGGAGAGCCTAATAACGT
GGGCGAGGAGGACTGTGCTGAGTTCAGCGGCAATGGCTGGAACGATGATAAATGCAACCTGGCCAAGTTT
TGGATCTGCAAAAAGTCAGCAGCAAGCTGTAGCAGAGACGAAGAACAGTTCCTGAGTCCCGCTCCCGCTA
CGCCTAATCCTCCGCCCGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC226695 representing NM_001144896
Red=Cloning site Green=Tags(s)

MSDSKEPRLQQLGLLEEEQLRGLGFRQTRGYKSLAVSKVPSSISQEQSRQDAIYQNLTQLKAAVGELSEK
SKLQEIYQELTQLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTWLKAAVGELPEKS
KMQEIYQELTRLKAAVGELPEKSKQQEIYQELTRLKAAVGELPEKSKQQEIYQELTRLKAAVGELPEKSK
QQEIYQELTQLKAAVERLCHPCPWEWTFFQGNCYFMSNSQRNWHDSITACKEVGAQLVVIKSAEEQNFLQ
LQSSRSNRFTWMGLSDLNQEGTWQWVDGSPLLPSFKQYWNRGEPNNVGEEDCAEFSGNGWNDDKCNLAKF
WICKKSAASCSRDEEQFLSPAPATPNPPPA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001144896
ORF Size 1140 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001144896.1, NP_001138368.1
RefSeq Size 4256 bp
RefSeq ORF 1143 bp
Locus ID 30835
UniProt ID Q9NNX6
Protein Families Druggable Genome
MW 43.3 kDa
Gene Summary This gene encodes a C-type lectin that functions in cell adhesion and pathogen recognition. This receptor recognizes a wide range of evolutionarily divergent pathogens with a large impact on public health, including leprosy and tuberculosis mycobacteria, the Ebola, hepatitis C, HIV-1 and Dengue viruses, and the SARS-CoV acute respiratory syndrome coronavirus. The protein is organized into four distinct domains: a C-terminal carbohydrate recognition domain, a flexible tandem-repeat neck domain, a transmembrane region and an N-terminal cytoplasmic domain involved in internalization. This gene is closely related in terms of both sequence and function to a neighboring gene, CLEC4M (Gene ID: 10332), also known as L-SIGN. The two genes differ in viral recognition and expression patterns, with this gene showing high expression on the surface of dendritic cells. Polymorphisms in the neck region are associated with protection from HIV-1 infection, while single nucleotide polymorphisms in the promoter of this gene are associated with differing resistance and susceptibility to and severity of infectious disease, including rs4804803, which is associated with SARS severity. [provided by RefSeq, May 2020]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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