HOMER3 (NM_001145722) Human Tagged ORF Clone

CAT#: RC226668

HOMER3 (Myc-DDK-tagged)-Human homer homolog 3 (Drosophila) (HOMER3), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_001145722" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal antibody to Homer-3 (homer homolog 3 (Drosophila))
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HOMER-3; VESL3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC226668 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACAGCCAGGGAGCAGCCAATCTTCAGCACACGGGCGCACGTGTTCCAAATTGACCCAGCCACCA
AGCGAAACTGGATCCCAGCGGGCAAGCACGCACTCACTGTCTCCTATTTCTACGATGCCACCCGCAATGT
GTACCGCATCATCAGCATCGGAGGCGCCAAGGCCATCATCAACAGCACTGTCACTCCCAACATGACCTTC
ACCAAAACTTCCCAGAAGTTCGGGCAGTGGGCCGACAGTCGCGCCAACACAGTCTACGGCTTGGGCTTTG
CCTCTGAACAGCATCTGACACAGTTTGCCGAGAAGTTCCAGGAAGTGAAGGAAGCAGCCAGGCTGGCCAG
GGAGAAATCTCAGGATGGCGGGGAGCTCACCAGTCCAGCCCTGGGGCTCGCCTCCCACCAGGTGCCCCCG
AGCCCTCTCGTCAGTGCCAACGGCCCCGGCGAGGAAAAACTGTTCCGCAGCCAGAGCGCTGATGCCCCCG
GCCCCACAGAGCGCGAGCGGCTAAAGAAGATGTTGTCTGAGGGCTCCGTGGGCGAGGTACAGTGGGAGGC
CGAGTTTTTCGCACTGCAGGACAGCAACAACAAGCTGGCAGGCGCCCTGCGAGAGGCCAACGCCGCCGCA
GCCCAGTGGAGGCAGCAGCTGGAGGCTCAGCGTGCAGAGGCCGAGCGGCTGCGGCAGCGGGTGGCTGAGC
TGGAGGCTCAGGCAGCTTCAGAGGTGACCCCCACCGGTGAGAAGGAGGGGCTGGGCCAGGGCCAGTCGCT
GGAACAGCTGGAAGCTCTGGTGCAAACCAAGGACCAGGAGATTCAGACCCTGAAGAGTCAGACTGGGGGG
CCCCGCGAGGCCCTGGAGGCTGCCGAGCGTGAGGAGACTCAGCAGAAGGTGCAGGACCTGGAGACCCGCA
ATGCGGAGTTGGAGCACCAGCTGCGGGCGATGGAGCGCAGCCTGGAGGAGGCACGGGCAGAGCGGGAGCG
GGCGCGGGCTGAGGTGGGCCGGGCAGCGCAGCTGCTGGACGTCAGGCTGTTTGAGCTGAGTGAGCTGCGT
GAGGGCCTGGCCCGCCTGGCTGAGGCTGCGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC226668 protein sequence
Red=Cloning site Green=Tags(s)

MSTAREQPIFSTRAHVFQIDPATKRNWIPAGKHALTVSYFYDATRNVYRIISIGGAKAIINSTVTPNMTF
TKTSQKFGQWADSRANTVYGLGFASEQHLTQFAEKFQEVKEAARLAREKSQDGGELTSPALGLASHQVPP
SPLVSANGPGEEKLFRSQSADAPGPTERERLKKMLSEGSVGEVQWEAEFFALQDSNNKLAGALREANAAA
AQWRQQLEAQRAEAERLRQRVAELEAQAASEVTPTGEKEGLGQGQSLEQLEALVQTKDQEIQTLKSQTGG
PREALEAAEREETQQKVQDLETRNAELEHQLRAMERSLEEARAERERARAEVGRAAQLLDVRLFELSELR
EGLARLAEAAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001145722
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001145722.1, NP_001139194.1
RefSeq Size 1859 bp
RefSeq ORF 1086 bp
Locus ID 9454
UniProt ID Q9NSC5
Protein Families Druggable Genome
MW 39.9 kDa
Gene Summary This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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