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BCMO1 (BCO1) (NM_017429) Human Tagged ORF Clone
CAT＃: RC223790
BCMO1 (Myc-DDK-tagged)-Human beta-carotene 15,15'-monooxygenase 1 (BCMO1)
ORF Plasmid: tGFP
"NM_017429" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BCDO; BCDO1; BCMO; BCMO1; BCO |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223790 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATAATATTTGGCAGGAATAGGAAAGAACAGCTGGAGCCTGTGAGGGCCAAAGTGACAGGCAAGA TTCCAGCATGGCTGCAGGGAACCCTGCTCCGCAACGGGCCTGGGATGCACACAGTTGGGGAGTCCAGATA CAACCATTGGTTCGACGGCCTTGCCCTGCTCCACAGCTTCACCATCAGAGACGGTGAAGTCTATTACAGG AGCAAATACCTGAGAAGCGATACCTACAACACCAATATTGAGGCAAACAGGATTGTGGTGTCTGAGTTTG GAACAATGGCCTATCCGGACCCCTGCAAAAACATATTTTCCAAAGCTTTCTCCTACTTGTCTCACACCAT CCCCGATTTCACCGACAACTGCCTGATCAACATCATGAAGTGCGGAGAAGACTTCTACGCGACCTCAGAG ACCAATTACATCAGGAAAATCAACCCACAGACTCTGGAAACCCTGGAGAAGGTTGATTATCGTAAATACG TGGCGGTAAATCTGGCAACGTCACATCCCCATTATGATGAGGCTGGAAATGTTCTAAACATGGGCACATC CATTGTGGAAAAGGGGAAGACAAAGTATGTGATTTTTAAGATCCCTGCCACAGTACCAGAGGGCAAGAAG CAGGGGAAGAGCCCCTGGAAGCACACAGAGGTGTTCTGCTCCATCCCATCCCGCTCCCTGCTCTCCCCAA GCTACTACCACAGCTTTGGAGTCACCGAGAACTATGTCATCTTCCTTGAGCAGCCTTTCAGGTTGGATAT TCTCAAGATGGCAACCGCATACATCCGGAGTATGAGCTGGGCCTCCTGCCTGGCTTTCCACAGGGAGGAG AAGACTTATATCCACATCATCGACCAAAGGACCAGGCAGCCTGTGCAGACCAAGTTTTACACAGACGCCA TGGTGGTCTTCCATCACGTCAACGCCTACGAAGAGGACGGCTGCATCGTGTTTGACGTCATTGCCTACGA GGACAACAGCCTCTACCAGCTCTTCTACCTGGCCAACCTGAACCAGGACTTCAAGGAGAACTCCAGGCTC ACCTCGGTCCCCACCCTCAGGAGGTTTGCCGTGCCCCTCCACGTGGACAAGAATGCAGAAGTGGGCACAA ATTTAATCAAAGTGGCATCTACAACAGCCACGGCCCTGAAGGAAGAAGATGGCCAAGTCTACTGCCAGCC GGAATTTCTTTATGAAGGCTTAGAGCTTCCACGGGTCAATTATGCTCACAATGGAAAGCAATACCGATAT GTCTTTGCTACAGGAGTTCAGTGGAGTCCAATCCCAACCAAGATAATAAAATATGACATTCTCACAAAGT CATCCTTAAAATGGAGAGAGGACGACTGCTGGCCAGCGGAACCCCTGTTTGTGCCCGCGCCAGGTGCCAA GGATGAGGATGACGGAGTAATCTTATCAGCCATTGTCTCTACTGATCCCCAAAAGCTGCCTTTTCTGCTC ATTCTGGATGCCAAAAGCTTTACGGAATTGGCCCGTGCCTCTGTTGATGTCGATATGCACATGGATCTCC ATGGATTATTCATTACAGACATGGACTGGGACACAAAAAAGCAGGCCGCTTCTGAGGAACAGCGGGACAG GGCTTCCGACTGCCACGGGGCTCCTCTGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223790 protein sequence
Red=Cloning site Green=Tags(s) MDIIFGRNRKEQLEPVRAKVTGKIPAWLQGTLLRNGPGMHTVGESRYNHWFDGLALLHSFTIRDGEVYYR SKYLRSDTYNTNIEANRIVVSEFGTMAYPDPCKNIFSKAFSYLSHTIPDFTDNCLINIMKCGEDFYATSE TNYIRKINPQTLETLEKVDYRKYVAVNLATSHPHYDEAGNVLNMGTSIVEKGKTKYVIFKIPATVPEGKK QGKSPWKHTEVFCSIPSRSLLSPSYYHSFGVTENYVIFLEQPFRLDILKMATAYIRSMSWASCLAFHREE KTYIHIIDQRTRQPVQTKFYTDAMVVFHHVNAYEEDGCIVFDVIAYEDNSLYQLFYLANLNQDFKENSRL TSVPTLRRFAVPLHVDKNAEVGTNLIKVASTTATALKEEDGQVYCQPEFLYEGLELPRVNYAHNGKQYRY VFATGVQWSPIPTKIIKYDILTKSSLKWREDDCWPAEPLFVPAPGAKDEDDGVILSAIVSTDPQKLPFLL ILDAKSFTELARASVDVDMHMDLHGLFITDMDWDTKKQAASEEQRDRASDCHGAPLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017429 |
| ORF Size | 1641 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017429.3 |
| RefSeq Size | 2446 bp |
| RefSeq ORF | 1644 bp |
| Locus ID | 53630 |
| UniProt ID | Q9HAY6 |
| Protein Pathways | Metabolic pathways, Retinol metabolism |
| MW | 62.6 kDa |
| Gene Summary | Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223790L3 | Lenti ORF clone of Human beta-carotene 15,15'-monooxygenase 1 (BCMO1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223790L4 | Lenti ORF clone of Human beta-carotene 15,15'-monooxygenase 1 (BCMO1), mGFP tagged |
CNY 5,890.00 |
|
| RG223790 | BCMO1 (tGFP-tagged) - Human beta-carotene 15,15'-monooxygenase 1 (BCMO1) |
CNY 4,370.00 |
|
| SC304462 | BCMO1 (untagged)-Human beta-carotene 15,15'-monooxygenase 1 (BCMO1) |
CNY 4,080.00 |
