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PIGO (NM_032634) Human Tagged ORF Clone
CAT＃: RC222262
- TrueORF®
PIGO (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 1
ORF Plasmid: tGFP
"NM_032634" in other vectors (5)
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CNY 7,584.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HPMRS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222262 representing NM_032634
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAAGCCTCAGTGTTGCTCTTCCTGGCCTGGGTCTGCTTCCTCTTCTACGCTGGCATTGCCCTCT TCACCAGTGGCTTCCTGCTCACCCGTTTGGAGCTCACCAACCATAGCAGCTGCCAAGAGCCCCCAGGCCC TGGGTCCCTGCCATGGGGGAGCCAAGGGAAACCTGGGGCCTGCTGGATGGCTTCCCGATTTTCGCGGGTT GTGTTGGTGCTGATAGATGCTCTGCGATTTGACTTCGCCCAGCCCCAGCATTCACACGTGCCTAGAGAGC CTCCTGTCTCCCTACCCTTCCTGGGCAAACTAAGCTCCTTGCAGAGGATCCTGGAGATTCAGCCCCACCA TGCCCGGCTCTACCGATCTCAGGTTGACCCTCCTACCACCACCATGCAGCGCCTCAAGGCCCTCACCACT GGCTCACTGCCTACCTTTATTGATGCTGGTAGTAACTTCGCCAGCCACGCCATAGTGGAAGACAATCTCA TTAAGCAGCTCACCAGTGCAGGAAGGCGTGTAGTCTTCATGGGAGATGATACCTGGAAAGACCTTTTCCC TGGTGCTTTCTCCAAAGCTTTCTTCTTCCCATCCTTCAATGTCAGAGACCTAGACACAGTGGACAATGGC ATCCTGGAACACCTCTACCCCACCATGGACAGTGGTGAATGGGACGTGCTGATTGCTCACTTCCTGGGTG TGGACCACTGTGGCCACAAGCATGGCCCTCACCACCCTGAAATGGCCAAGAAACTTAGCCAGATGGACCA GGTGATCCAGGGACTTGTGGAGCGTCTGGAGAATGACACACTGCTGGTAGTGGCTGGGGACCATGGGATG ACCACAAATGGAGACCATGGAGGGGACAGTGAGCTGGAGGTCTCAGCTGCTCTCTTTCTGTATAGCCCCA CAGCAGTCTTCCCCAGCACCCCACCAGAGGAGCCAGAGGTGATTCCTCAAGTTAGCCTTGTGCCCACGCT GGCCCTGCTGCTGGGCCTGCCCATCCCATTTGGGAATATCGGGGAAGTGATGGCTGAGCTATTCTCAGGG GGTGAGGACTCCCAGCCCCACTCCTCTGCTTTAGCCCAAGCCTCAGCTCTCCATCTCAATGCTCAGCAGG TGTCCCGATTTCTTCATACCTACTCAGCTGCTACTCAGGACCTTCAAGCTAAGGAGCTTCATCAGCTGCA GAACCTCTTCTCCAAGGCCTCTGCTGACTACCAGTGGCTTCTCCAGAGCCCCAAGGGGGCTGAGGCGACA CTGCCGACTGTGATTGCTGAGCTGCAGCAGTTCCTGCGGGGAGCTCGGGCCATGTGCATCGAGTCTTGGG CTCGTTTCTCTCTGGTCCGCATGGCGGGGGGTACTGCTCTCTTGGCTGCTTCCTGCTTTATCTGCCTGCT GGCATCTCAGTGGGCAATATCCCCAGGCTTTCCATTCTGCCCTCTACTCCTGACACCTGTGGCCTGGGGC CTGGTTGGGGCCATAGCGTATGCTGGACTCCTGGGAACTATTGAGCTGAAGCTAGATCTAGTGCTTCTAG GGGCTGTGGCTGCAGTGAGCTCATTCCTCCCTTTTCTGTGGAAAGCCTGGGCTGGCTGGGGGTCCAAGAG GCCCCTGGCAACCCTGTTTCCCATCCCTGGGCCCGTCCTGTTACTCCTGCTGTTTCGCTTGGCTGTGTTC TTCTCTGATAGTTTTGTTGTAGCTGAGGCCAGGGCCACCCCCTTCCTTTTGGGCTCATTCATCCTGCTCC TGGTTGTCCAGCTTCACTGGGAGGGCCAGCTGCTTCCACCTAAGCTACTCACAATGCCCCGCCTTGGCAC TTCAGCCACAACAAACCCCCCACGGCACAATGGTGCATATGCCCTGAGGCTTGGAATTGGGTTGCTTTTA TGTACAAGGCTAGCTGGGCTTTTTCATCGTTGCCCTGAAGAGACACCTGTTTGCCACTCCTCTCCCTGGC TGAGTCCTCTGGCATCCATGGTGGGTGGTCGAGCCAAGAATTTGTGGTATGGAGCTTGTGTGGCGGCGCT GGTGGCCCTGTTAGCTGCCGTGCGCTTGTGGCTTCGCCGCTATGGTAATCTCAAGAGCCCCGAGCCACCC ATGCTCTTTGTGCGCTGGGGACTGCCCCTAATGGCATTGGGTACTGCTGCCTACTGGGCATTGGCGTCGG GGGCAGATGAGGCTCCCCCCCGTCTCCGGGTCCTGGTCTCTGGGGCATCCATGGTGCTGCCTCGGGCTGT AGCAGGGCTGGCTGCTTCAGGGCTCGCGCTGCTGCTCTGGAAGCCTGTGACAGTGCTGGTGAAGGCTGGG GCAGGCGCTCCAAGGACCAGGACTGTCCTCACTCCCTTCTCAGGCCCCCCCACTTCTCAAGCTGACTTGG ATTATGTGGTCCCTCAAATCTACCGACACATGCAGGAGGAGTTCCGGGGCCGGTTAGAGAGGACCAAATC TCAGGGTCCCCTGACTGTGGCTGCTTATCAGTTGGGGAGTGTCTACTCAGCTGCTATGGTCACAGCCCTC ACCCTGTTGGCCTTCCCACTTCTGCTGTTGCATGCGGAGCGCATCAGCCTTGTGTTCCTGCTTCTGTTTC TGCAGAGCTTCCTTCTCCTACATCTGCTTGCTGCTGGGATACCCGTCACCACCCCTGGTCCTTTTACTGT GCCATGGCAGGCAGTCTCGGCTTGGGCCCTCATGGCCACACAGACCTTCTACTCCACAGGCCACCAGCCT GTCTTTCCAGCCATCCATTGGCATGCAGCCTTCGTGGGATTCCCAGAGGGTCATGGCTCCTGTACTTGGC TGCCTGCTTTGCTAGTGGGAGCCAACACCTTTGCCTCCCACCTCCTCTTTGCAGTAGGTTGCCCACTGCT CCTGCTCTGGCCTTTCCTGTGTGAGAGTCAAGGGCTGCGGAAGAGACAGCAGCCCCCAGGGAATGAAGCT GATGCCAGAGTCAGACCCGAGGAGGAAGAGGAGCCACTGATGGAGATGCGGCTCCGGGATGCGCCTCAGC ACTTCTATGCAGCACTGCTGCAGCTGGGCCTCAAGTACCTCTTTATCCTTGGTATTCAGATTCTGGCCTG TGCCTTGGCAGCCTCCATCCTTCGCAGGCATCTCATGGTCTGGAAAGTGTTTGCCCCTAAGTTCATATTT GAGGCTGTGGGCTTCATTGTGAGCAGCGTGGGACTTCTCCTGGGCATAGCTTTGGTGATGAGAGTGGATG GTGCTGTGAGCTCCTGGTTCAGGCAGCTATTTCTGGCCCAGCAGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222262 representing NM_032634
Red=Cloning site Green=Tags(s) MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGACWMASRFSRV VLVLIDALRFDFAQPQHSHVPREPPVSLPFLGKLSSLQRILEIQPHHARLYRSQVDPPTTTMQRLKALTT GSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSFNVRDLDTVDNG ILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGM TTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSG GEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEAT LPTVIAELQQFLRGARAMCIESWARFSLVRMAGGTALLAASCFICLLASQWAISPGFPFCPLLLTPVAWG LVGAIAYAGLLGTIELKLDLVLLGAVAAVSSFLPFLWKAWAGWGSKRPLATLFPIPGPVLLLLLFRLAVF FSDSFVVAEARATPFLLGSFILLLVVQLHWEGQLLPPKLLTMPRLGTSATTNPPRHNGAYALRLGIGLLL CTRLAGLFHRCPEETPVCHSSPWLSPLASMVGGRAKNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPP MLFVRWGLPLMALGTAAYWALASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLVKAG AGAPRTRTVLTPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQLGSVYSAAMVTAL TLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPVTTPGPFTVPWQAVSAWALMATQTFYSTGHQP VFPAIHWHAAFVGFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEA DARVRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILACALAASILRRHLMVWKVFAPKFIF EAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQLFLAQQR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032634 |
| ORF Size | 3267 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032634.4 |
| RefSeq Size | 4076 bp |
| RefSeq ORF | 3270 bp |
| Locus ID | 84720 |
| UniProt ID | Q8TEQ8 |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
| MW | 118.5 kDa |
| Gene Summary | This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least three alternatively spliced transcripts encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222262L3 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 1, Myc-DDK-tagged |
CNY 12,730.00 |
|
| RC222262L4 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 1, mGFP tagged |
CNY 12,730.00 |
|
| RG222262 | PIGO (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 1 |
CNY 11,880.00 |
|
| SC111852 | PIGO (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 1 |
CNY 3,656.00 |
|
| SC318490 | PIGO (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 1 |
CNY 7,592.00 |
