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TLR8 (NM_138636) Human Tagged ORF Clone
CAT＃: RC221986
- TrueORF®
TLR8 (Myc-DDK-tagged)-Human toll-like receptor 8 (TLR8)
ORF Plasmid: tGFP
"NM_138636" in other vectors (6)
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CNY 11,600.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD288 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221986 representing NM_138636
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACATGTTCCTTCAGTCGTCAATGCTGACCTGCATTTTCCTGCTAATATCTGGTTCCTGTGAGT TATGCGCCGAAGAAAATTTTTCTAGAAGCTATCCTTGTGATGAGAAAAAGCAAAATGACTCAGTTATTGC AGAGTGCAGCAATCGTCGACTACAGGAAGTTCCCCAAACGGTGGGCAAATATGTGACAGAACTAGACCTG TCTGATAATTTCATCACACACATAACGAATGAATCATTTCAAGGGCTGCAAAATCTCACTAAAATAAATC TAAACCACAACCCCAATGTACAGCACCAGAACGGAAATCCCGGTATACAATCAAATGGCTTGAATATCAC AGACGGGGCATTCCTCAACCTAAAAAACCTAAGGGAGTTACTGCTTGAAGACAACCAGTTACCCCAAATA CCCTCTGGTTTGCCAGAGTCTTTGACAGAACTTAGTCTAATTCAAAACAATATATACAACATAACTAAAG AGGGCATTTCAAGACTTATAAACTTGAAAAATCTCTATTTGGCCTGGAACTGCTATTTTAACAAAGTTTG CGAGAAAACTAACATAGAAGATGGAGTATTTGAAACGCTGACAAATTTGGAGTTGCTATCACTATCTTTC AATTCTCTTTCACACGTGCCACCCAAACTGCCAAGCTCCCTACGCAAACTTTTTCTGAGCAACACCCAGA TCAAATACATTAGTGAAGAAGATTTCAAGGGATTGATAAATTTAACATTACTAGATTTAAGCGGGAACTG TCCGAGGTGCTTCAATGCCCCATTTCCATGCGTGCCTTGTGATGGTGGTGCTTCAATTAATATAGATCGT TTTGCTTTTCAAAACTTGACCCAACTTCGATACCTAAACCTCTCTAGCACTTCCCTCAGGAAGATTAATG CTGCCTGGTTTAAAAATATGCCTCATCTGAAGGTGCTGGATCTTGAATTCAACTATTTAGTGGGAGAAAT AGCCTCTGGGGCATTTTTAACGATGCTGCCCCGCTTAGAAATACTTGACTTGTCTTTTAACTATATAAAG GGGAGTTATCCACAGCATATTAATATTTCCAGAAACTTCTCTAAACTTTTGTCTCTACGGGCATTGCATT TAAGAGGTTATGTGTTCCAGGAACTCAGAGAAGATGATTTCCAGCCCCTGATGCAGCTTCCAAACTTATC GACTATCAACTTGGGTATTAATTTTATTAAGCAAATCGATTTCAAACTTTTCCAAAATTTCTCCAATCTG GAAATTATTTACTTGTCAGAAAACAGAATATCACCGTTGGTAAAAGATACCCGGCAGAGTTATGCAAATA GTTCCTCTTTTCAACGTCATATCCGGAAACGACGCTCAACAGATTTTGAGTTTGACCCACATTCGAACTT TTATCATTTCACCCGTCCTTTAATAAAGCCACAATGTGCTGCTTATGGAAAAGCCTTAGATTTAAGCCTC AACAGTATTTTCTTCATTGGGCCAAACCAATTTGAAAATCTTCCTGACATTGCCTGTTTAAATCTGTCTG CAAATAGCAATGCTCAAGTGTTAAGTGGAACTGAATTTTCAGCCATTCCTCATGTCAAATATTTGGATTT GACAAACAATAGACTAGACTTTGATAATGCTAGTGCTCTTACTGAATTGTCCGACTTGGAAGTTCTAGAT CTCAGCTATAATTCACACTATTTCAGAATAGCAGGCGTAACACATCATCTAGAATTTATTCAAAATTTCA CAAATCTAAAAGTTTTAAACTTGAGCCACAACAACATTTATACTTTAACAGATAAGTATAACCTGGAAAG CAAGTCCCTGGTAGAATTAGTTTTCAGTGGCAATCGCCTTGACATTTTGTGGAATGATGATGACAACAGG TATATCTCCATTTTCAAAGGTCTCAAGAATCTGACACGTCTGGATTTATCCCTTAATAGGCTGAAGCACA TCCCAAATGAAGCATTCCTTAATTTGCCAGCGAGTCTCACTGAACTACATATAAATGATAATATGTTAAA GTTTTTTAACTGGACATTACTCCAGCAGTTTCCTCGTCTCGAGTTGCTTGACTTACGTGGAAACAAACTA CTCTTTTTAACTGATAGCCTATCTGACTTTACATCTTCCCTTCGGACACTGCTGCTGAGTCATAACAGGA TTTCCCACCTACCCTCTGGCTTTCTTTCTGAAGTCAGTAGTCTGAAGCACCTCGATTTAAGTTCCAATCT GCTAAAAACAATCAACAAATCCGCACTTGAAACTAAGACCACCACCAAATTATCTATGTTGGAACTACAC GGAAACCCCTTTGAATGCACCTGTGACATTGGAGATTTCCGAAGATGGATGGATGAACATCTGAATGTCA AAATTCCCAGACTGGTAGATGTCATTTGTGCCAGTCCTGGGGATCAAAGAGGGAAGAGTATTGTGAGTCT GGAGCTAACAACTTGTGTTTCAGATGTCACTGCAGTGATATTATTTTTCTTCACGTTCTTTATCACCACC ATGGTTATGTTGGCTGCCCTGGCTCACCATTTGTTTTACTGGGATGTTTGGTTTATATATAATGTGTGTT TAGCTAAGGTAAAAGGCTACAGGTCTCTTTCCACATCCCAAACTTTCTATGATGCTTACATTTCTTATGA CACCAAAGATGCCTCTGTTACTGACTGGGTGATAAATGAGCTGCGCTACCACCTTGAAGAGAGCCGAGAC AAAAACGTTCTCCTTTGTCTAGAGGAGAGGGATTGGGATCCGGGATTGGCCATCATCGACAACCTCATGC AGAGCATCAACCAAAGCAAGAAAACAGTATTTGTTTTAACCAAAAAATATGCAAAAAGCTGGAACTTTAA AACAGCTTTTTACTTGGCTTTGCAGAGGCTAATGGATGAGAACATGGATGTGATTATATTTATCCTGCTG GAGCCAGTGTTACAGCATTCTCAGTATTTGAGGCTACGGCAGCGGATCTGTAAGAGCTCCATCCTCCAGT GGCCTGACAACCCGAAGGCAGAAGGCTTGTTTTGGCAAACTCTGAGAAATGTGGTCTTGACTGAAAATGA TTCACGGTATAACAATATGTATGTCGATTCCATTAAGCAATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221986 representing NM_138636
Red=Cloning site Green=Tags(s) MENMFLQSSMLTCIFLLISGSCELCAEENFSRSYPCDEKKQNDSVIAECSNRRLQEVPQTVGKYVTELDL SDNFITHITNESFQGLQNLTKINLNHNPNVQHQNGNPGIQSNGLNITDGAFLNLKNLRELLLEDNQLPQI PSGLPESLTELSLIQNNIYNITKEGISRLINLKNLYLAWNCYFNKVCEKTNIEDGVFETLTNLELLSLSF NSLSHVPPKLPSSLRKLFLSNTQIKYISEEDFKGLINLTLLDLSGNCPRCFNAPFPCVPCDGGASINIDR FAFQNLTQLRYLNLSSTSLRKINAAWFKNMPHLKVLDLEFNYLVGEIASGAFLTMLPRLEILDLSFNYIK GSYPQHINISRNFSKLLSLRALHLRGYVFQELREDDFQPLMQLPNLSTINLGINFIKQIDFKLFQNFSNL EIIYLSENRISPLVKDTRQSYANSSSFQRHIRKRRSTDFEFDPHSNFYHFTRPLIKPQCAAYGKALDLSL NSIFFIGPNQFENLPDIACLNLSANSNAQVLSGTEFSAIPHVKYLDLTNNRLDFDNASALTELSDLEVLD LSYNSHYFRIAGVTHHLEFIQNFTNLKVLNLSHNNIYTLTDKYNLESKSLVELVFSGNRLDILWNDDDNR YISIFKGLKNLTRLDLSLNRLKHIPNEAFLNLPASLTELHINDNMLKFFNWTLLQQFPRLELLDLRGNKL LFLTDSLSDFTSSLRTLLLSHNRISHLPSGFLSEVSSLKHLDLSSNLLKTINKSALETKTTTKLSMLELH GNPFECTCDIGDFRRWMDEHLNVKIPRLVDVICASPGDQRGKSIVSLELTTCVSDVTAVILFFFTFFITT MVMLAALAHHLFYWDVWFIYNVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRYHLEESRD KNVLLCLEERDWDPGLAIIDNLMQSINQSKKTVFVLTKKYAKSWNFKTAFYLALQRLMDENMDVIIFILL EPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQTLRNVVLTENDSRYNNMYVDSIKQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138636 |
| ORF Size | 3123 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138636.4 |
| RefSeq Size | 4211 bp |
| RefSeq ORF | 3126 bp |
| Locus ID | 51311 |
| UniProt ID | Q9NR97 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Toll-like receptor signaling pathway |
| MW | 119.6 kDa |
| Gene Summary | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Toll-Like Receptor 8 Agonist Strengthens the Protective Efficacy of ESAT-6 Immunization toMycobacterium tuberculosisInfection
,Tang, J;Sun, M;Shi, G;Xu, Y;Han, Y;Li, X;Dong, W;Zhan, L;Qin, C;,
Front Immunol
,PubMed ID 29416532
[TLR8]
|
|
PNAS Plus: MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory response
,Muller Fabbri, Alessio Paone, Federica Calore, Roberta Galli, Eugenio Gaudio, Ramasamy Santhanam, Francesca Lovat, Paolo Fadda, Charlene Mao, Gerard J. Nuovo, Nicola Zanesi, Melissa Crawford, Gulcin H. Ozer, Dorothee Wernicke, Hansjuerg Alder, Michael A. Caligiuri, Patrick Nana-Sinkam, Danilo Perrotti, and Carlo M. Croce,
PNAS, Jul 2012; 109: E2110 - E2116.
[TLR8]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221986L1 | Lenti ORF clone of Human toll-like receptor 8 (TLR8), Myc-DDK-tagged |
CNY 14,000.00 |
|
| RC221986L2 | Lenti ORF clone of Human toll-like receptor 8 (TLR8), mGFP tagged |
CNY 12,260.00 |
|
| RC221986L3 | Lenti ORF clone of Human toll-like receptor 8 (TLR8), Myc-DDK-tagged |
CNY 12,260.00 |
|
| RC221986L4 | Lenti ORF clone of Human toll-like receptor 8 (TLR8), mGFP tagged |
CNY 14,000.00 |
|
| RG221986 | TLR8 (tGFP-tagged) - Human toll-like receptor 8 (TLR8) |
CNY 13,200.00 |
|
| SC306054 | TLR8 (untagged)-Human toll-like receptor 8 (TLR8) |
CNY 10,880.00 |
