NRG2 (NM_013983) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DON1; HRG2; NTAK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC221873 representing NM_013983 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCAGGTTTGCTGCTCAGCGCTGCCGCCGCCGCCACTGGAGAAGGGTCGGTGCAGCAGCTACAGCG ACAGCAGCAGCAGCAGCAGCGAGAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCGAGAGCGGCAGCAGCAG CAGGAGCAGCAGCAACAACAGCAGCATCTCTCGTCCCGCTGCGCCCCCAGAGCCGCGGCCGCAGCAACAG CCGCAGCCCCGCAGCCCCGCAGCCCGGAGAGCCGCCGCCCGTTCGCGAGCCGCAGCCGCCGGCGGCATGA GGCGCGACCCGGCCCCCGGCTTCTCCATGCTGCTCTTCGGTGTGTCGCTCGCCTGCTACTCGCCCAGCCT CAAGTCAGTGCAGGACCAGGCGTACAAGGCACCCGTGGTGGTGGAGGGCAAGGTACAGGGGCTGGTCCCA GCCGGCGGCTCCAGCTCCAACAGCACCCGAGAGCCGCCCGCCTCGGGTCGGGTGGCGTTGGTAAAGGTGC TGGACAAGTGGCCGCTCCGGAGCGGGGGGCTGCAGCGCGAGCAGGTGATCAGCGTGGGCTCCTGTGTGCC GCTCGAAAGGAACCAGCGCTACATCTTTTTCCTGGAGCCCACGGAACAGCCCTTAGTCTTTAAGACGGCC TTTGCCCCCCTCGATACCAACGGCAAAAATCTCAAGAAAGAGGTGGGCAAGATCCTGTGCACTGACTGCG CCACCCGGCCCAAGTTGAAGAAGATGAAGAGCCAGACGGGACAGGTGGGTGAGAAGCAATCGCTGAAGTG TGAGGCAGCAGCCGGTAATCCCCAGCCTTCCTACCGTTGGTTCAAGGATGGCAAGGAGCTCAACCGCAGC CGAGACATTCGCATCAAATATGGCAACGGCAGAAAGAACTCACGACTACAGTTCAACAAGGTGAAGGTGG AGGACGCTGGGGAGTATGTCTGCGAGGCCGAGAACATCCTGGGGAAGGACACCGTCCGGGGCCGGCTTTA CGTCAACAGCGTGAGCACCACCCTGTCATCCTGGTCGGGGCACGCCCGGAAGTGCAACGAGACAGCCAAG TCCTATTGCGTCAATGGAGGCGTCTGCTACTACATCGAGGGCATCAACCAGCTCTCCTGCAAGTGTCCTG TGGGATACACCGGGGACAGGTGTCAGCAGTTCGCAATGGTCAACTTCTCCAAGCACCTTGGATTTGAATT AAAGGAAGCCGAGGAGCTGTACCAGAAGAGGGTCCTGACCATCACGGGCATCTGCGTGGCTCTGCTGGTC GTGGGCATCGTCTGTGTGGTGGCCTACTGCAAGACCAAAAAACAGCGGAAGCAGATGCACAACCACCTCC GGCAGAACATGTGCCCGGCCCATCAGAACCGGAGCTTGGCCAATGGGCCCAGCCACCCCCGGCTGGACCC AGAGGAGATCCAGATGGCAGATTATATTTCCAAGAACGTGCCAGCCACAGACCATGTCATCAGGAGAGAA ACTGAGACCACCTTCTCTGGGAGCCACTCCTGTTCTCCTTCTCACCACTGCTCCACAGCCACACCCACCT CCAGCCACAGACACGAGAGCCACACGTGGAGCCTGGAACGTTCTGAGAGCCTGACTTCTGACTCCCAGTC GGGGATCATGCTATCATCAGTGGGTACCAGCAAATGCAACAGCCCAGCATGTGTGGAGGCCCGGGCAAGG CGGGCAGCAGCCTACAACCTGGAGGAGCGGCGCAGGGCCACCGCGCCACCCTATCACGATTCCGTGGACT CCCTTCGCGACTCCCCACACAGCGAGAGGTACGTGTCGGCCCTGACCACGCCCGCGCGCCTCTCGCCCGT GGACTTCCACTACTCGCTGGCCACGCAGGTGCCAACTTTCGAGATCACGTCCCCCAACTCGGCGCACGCC GTGTCGCTGCCGCCGGCGGCGCCCATCAGTTACCGCCTGGCCGAGCAGCAGCCGTTACTGCGGCACCCGG CGCCCCCCGGCCCGGGACCCGGACCCGGGCCCGGGCCCGGGCCCGGCGCAGACATGCAGCGCAGCTATGA CAGCTACTATTACCCCGCGGCGGGGCCCGGACCGCGGCGCGGGACCTGCGCGCTCGGCGGCAGCCTGGGC AGCCTGCCTGCCAGCCCCTTCCGCATCCCCGAGGACGACGAGTACGAGACCACGCAGGAGTGCGCGCCCC CGCCGCCGCCGCGGCCGCGCGCGCGCGGTGCGTCCCGCAGGACGTCGGCGGGGCCCCGGCGCTGGCGCCG CTCGCGCCTCAACGGGCTGGCGGCGCAGCGCGCACGGGCGGCGAGGGACTCGCTGTCGCTGAGCAGCGGC TCGGGCGGCGGCTCAGCCTCGGCGTCGGACGACGACGCGGACGACGCGGACGGGGCGCTGGCGGCCGAGA GCACACCTTTCCTGGGCCTGCGTGGGGCGCACGACGCGCTGCGCTCGGACTCGCCGCCACTGTGCCCGGC GGCCGACAGCAGGACTTACTACTCACTGGACAGCCACAGCACGCGGGCCAGCAGCAGACACAGCCGCGGG CCGCCCCCGCGGGCCAAGCAGGACTCGGCGCCACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC221873 representing NM_013983 Red=Cloning site Green=Tags(s) MRQVCCSALPPPPLEKGRCSSYSDSSSSSSERSSSSSSSSSESGSSSRSSSNNSSISRPAAPPEPRPQQQ PQPRSPAARRAAARSRAAAAGGMRRDPAPGFSMLLFGVSLACYSPSLKSVQDQAYKAPVVVEGKVQGLVP AGGSSSNSTREPPASGRVALVKVLDKWPLRSGGLQREQVISVGSCVPLERNQRYIFFLEPTEQPLVFKTA FAPLDTNGKNLKKEVGKILCTDCATRPKLKKMKSQTGQVGEKQSLKCEAAAGNPQPSYRWFKDGKELNRS RDIRIKYGNGRKNSRLQFNKVKVEDAGEYVCEAENILGKDTVRGRLYVNSVSTTLSSWSGHARKCNETAK SYCVNGGVCYYIEGINQLSCKCPVGYTGDRCQQFAMVNFSKHLGFELKEAEELYQKRVLTITGICVALLV VGIVCVVAYCKTKKQRKQMHNHLRQNMCPAHQNRSLANGPSHPRLDPEEIQMADYISKNVPATDHVIRRE TETTFSGSHSCSPSHHCSTATPTSSHRHESHTWSLERSESLTSDSQSGIMLSSVGTSKCNSPACVEARAR RAAAYNLEERRRATAPPYHDSVDSLRDSPHSERYVSALTTPARLSPVDFHYSLATQVPTFEITSPNSAHA VSLPPAAPISYRLAEQQPLLRHPAPPGPGPGPGPGPGPGADMQRSYDSYYYPAAGPGPRRGTCALGGSLG SLPASPFRIPEDDEYETTQECAPPPPPRPRARGASRRTSAGPRRWRRSRLNGLAAQRARAARDSLSLSSG SGGGSASASDDDADDADGALAAESTPFLGLRGAHDALRSDSPPLCPAADSRTYYSLDSHSTRASSRHSRG PPPRAKQDSAPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013983
ORF Size	2556 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013983.3
RefSeq Size	3945 bp
RefSeq ORF	2559 bp
Locus ID	9542
UniProt ID	O14511
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	ErbB signaling pathway
MW	91.8 kDa
Gene Summary	This gene encodes a novel member of the neuregulin family of growth and differentiation factors. Through interaction with the ERBB family of receptors, this protein induces the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1, another member of the neuregulin family of ligands. The products of these genes mediate distinct biological processes by acting at different sites in tissues and eliciting different biological responses in cells. This gene is located close to the region for demyelinating Charcot-Marie-Tooth disease locus, but is not responsible for this disease. Alternative transcript variants encoding distinct isoforms have been described. [provided by RefSeq, May 2010]

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