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NOTCH4 (NM_004557) Human Tagged ORF Clone
CAT＃: RC221762
- TrueORF®
NOTCH4 (Myc-DDK-tagged)-Human notch 4 (NOTCH4)
ORF Plasmid: tGFP
"NM_004557" in other vectors (3)
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CNY 19,832.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | INT3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221762 representing NM_004557
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCCCCTTCACTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTATGTGTCTCAGTGGTCAGACCCA GAGGGCTGCTGTGTGGGAGTTTCCCAGAACCCTGTGCCAATGGAGGCACCTGCCTGAGCCTGTCTCTGGG ACAAGGGACCTGCCAGTGTGCCCCTGGCTTCCTGGGTGAGACGTGCCAGTTTCCTGACCCCTGCCAGAAC GCCCAGCTCTGCCAAAATGGAGGCAGCTGCCAAGCCCTGCTTCCCGCTCCCCTAGGGCTCCCCAGCTCTC CCTCTCCATTGACACCCAGCTTCTTGTGCACTTGCCTCCCTGGCTTCACTGGTGAGAGATGCCAGGCCAA GCTTGAAGACCCTTGTCCTCCCTCCTTCTGTTCCAAAAGGGGCCGCTGCCACATCCAGGCCTCGGGCCGC CCACAGTGCTCCTGCATGCCTGGATGGACAGGTGAGCAGTGCCAGCTTCGGGACTTCTGTTCAGCCAACC CATGTGTTAATGGAGGGGTGTGTCTGGCCACATACCCCCAGATCCAGTGCCACTGCCCACCGGGCTTCGA GGGCCATGCCTGTGAACGTGATGTCAACGAGTGCTTCCAGGACCCAGGACCCTGCCCCAAAGGCACCTCC TGCCATAACACCCTGGGCTCCTTCCAGTGCCTCTGCCCTGTGGGGCAGGAGGGTCCACGTTGTGAGCTGC GGGCAGGACCCTGCCCTCCTAGGGGCTGTTCGAATGGGGGCACCTGCCAGCTGATGCCAGAGAAAGACTC CACCTTTCACCTCTGCCTCTGTCCCCCAGGTTTCATAGGCCCAGACTGTGAGGTGAATCCAGACAACTGT GTCAGCCACCAGTGTCAGAATGGGGGCACTTGCCAGGATGGGCTGGACACCTACACCTGCCTCTGCCCAG AAACCTGGACAGGCTGGGACTGCTCCGAAGATGTGGATGAGTGTGAGACCCAGGGTCCCCCTCACTGCAG AAACGGGGGCACCTGCCAGAACTCTGCTGGTAGCTTTCACTGCGTGTGTGTGAGTGGCTGGGGCGGCACA AGCTGTGAGGAGAACCTGGATGACTGTATTGCTGCCACCTGTGCCCCGGGATCCACCTGCATTGACCGGG TGGGCTCTTTCTCCTGCCTCTGCCCACCTGGACGCACAGGACTCCTGTGCCACTTGGAAGACATGTGTCT GAGCCAGCCGTGCCATGGGGATGCCCAATGCAGCACCAACCCCCTCACAGGCTCCACACTCTGCCTGTGT CAGCCTGGCTATTCGGGGCCCACCTGCCACCAGGACCTGGACGAGTGTCTGATGGCCCAGCAAGGCCCAA GTCCCTGTGAACATGGCGGTTCCTGCCTCAACACTCCTGGCTCCTTCAACTGCCTCTGTCCACCTGGCTA CACAGGCTCCCGTTGTGAGGCTGATCACAATGAGTGCCTCTCCCAGCCCTGCCACCCAGGAAGCACCTGT CTGGACCTACTTGCCACCTTCCACTGCCTCTGCCCGCCAGGCTTAGAAGGGCAGCTCTGTGAGGTGGAGA CCAACGAGTGTGCCTCAGCTCCCTGCCTGAACCACGCGGATTGCCATGACCTGCTCAACGGCTTCCAGTG CATCTGCCTGCCTGGATTCTCCGGCACCCGATGTGAGGAGGATATCGATGAGTGCAGAAGCTCTCCCTGT GCCAATGGTGGGCAGTGCCAGGACCAGCCTGGAGCCTTCCACTGCAAGTGTCTCCCAGGCTTTGAAGGGC CACGCTGTCAAACAGAGGTGGATGAGTGCCTGAGTGACCCATGTCCCGTTGGAGCCAGCTGCCTTGATCT TCCAGGAGCCTTCTTTTGCCTCTGCCCCTCTGGTTTCACAGGCCAGCTCTGTGAGGTTCCCCTGTGTGCT CCCAACCTGTGCCAGCCCAAGCAGATATGTAAGGACCAGAAAGACAAGGCCAACTGCCTCTGTCCTGATG GAAGCCCTGGCTGTGCCCCACCTGAGGACAACTGCACCTGCCACCACGGGCACTGCCAGAGATCCTCATG TGTGTGTGACGTGGGTTGGACGGGGCCAGAGTGTGAGGCAGAGCTAGGGGGCTGCATCTCTGCACCCTGT GCCCATGGGGGGACCTGCTACCCCCAGCCCTCTGGCTACAACTGCACCTGCCCTACAGGCTACACAGGAC CCACCTGTAGTGAGGAGATGACAGCTTGTCACTCAGGGCCATGTCTCAATGGCGGCTCCTGCAACCCTAG CCCTGGAGGCTACTACTGCACCTGCCCTCCAAGCCACACAGGGCCCCAGTGCCAAACCAGCACTGACTAC TGTGTGTCTGCCCCGTGCTTCAATGGGGGTACCTGTGTGAACAGGCCTGGCACCTTCTCCTGCCTCTGTG CCATGGGCTTCCAGGGCCCGCGCTGTGAGGGAAAGCTCCGCCCCAGCTGTGCAGACAGCCCCTGTAGGAA TAGGGCAACCTGCCAGGACAGCCCTCAGGGTCCCCGCTGCCTCTGCCCCACTGGCTACACCGGAGGCAGC TGCCAGACTCTGATGGACTTATGTGCCCAGAAGCCCTGCCCACGCAATTCCCACTGCCTCCAGACTGGGC CCTCCTTCCACTGCTTGTGCCTCCAGGGATGGACCGGGCCTCTCTGCAACCTTCCACTGTCCTCCTGCCA GAAGGCTGCACTGAGCCAAGGCATAGACGTCTCTTCCCTTTGCCACAATGGAGGCCTCTGTGTCGACAGC GGCCCCTCCTATTTCTGCCACTGCCCCCCTGGATTCCAAGGCAGCCTGTGCCAGGATCACGTGAACCCAT GTGAGTCCAGGCCTTGCCAGAACGGGGCCACCTGCATGGCCCAGCCCAGTGGGTATCTCTGCCAGTGTGC CCCAGGCTACGATGGACAGAACTGCTCAAAGGAACTCGATGCTTGTCAGTCCCAACCCTGTCACAACCAT GGAACCTGTACTCCCAAACCTGGAGGATTCCACTGTGCCTGCCCTCCAGGCTTTGTGGGGCTACGCTGTG AGGGAGACGTGGACGAGTGTCTGGACCAGCCCTGCCACCCCACAGGCACTGCAGCCTGCCACTCTCTGGC CAATGCCTTCTACTGCCAGTGTCTGCCTGGACACACAGGCCAGTGGTGTGAGGTGGAGATAGACCCCTGC CACAGCCAACCCTGCTTTCATGGAGGGACCTGTGAGGCCACAGCAGGATCACCCCTGGGTTTCATCTGCC ACTGCCCCAAGGGTTTTGAAGGCCCCACCTGCAGCCACAGGGCCCCTTCCTGCGGCTTCCATCACTGCCA CCACGGAGGCCTGTGTCTGCCCTCCCCTAAGCCAGGCTTCCCACCACGCTGTGCCTGCCTCAGTGGCTAT GGGGGTCCTGACTGCCTGACCCCACCAGCTCCTAAAGGCTGTGGCCCTCCCTCCCCATGCCTATACAATG GCAGCTGCTCAGAGACCACGGGCTTGGGGGGCCCAGGCTTTCGATGCTCCTGCCCTCACAGCTCTCCAGG GCCCCGGTGTCAGAAACCCGGAGCCAAGGGGTGTGAGGGCAGAAGTGGAGATGGGGCCTGCGATGCTGGC TGCAGTGGCCCGGGAGGAAACTGGGATGGAGGGGACTGCTCTCTGGGAGTCCCAGACCCCTGGAAGGGCT GCCCCTCCCACTCTCGGTGCTGGCTTCTCTTCCGGGACGGGCAGTGCCACCCACAGTGTGACTCTGAAGA GTGTCTGTTTGATGGCTACGACTGTGAGACCCCTCCAGCCTGCACTCCAGCCTATGACCAGTACTGCCAT GATCACTTCCACAACGGGCACTGTGAGAAAGGCTGCAACACTGCAGAGTGTGGCTGGGATGGAGGTGACT GCAGGCCTGAAGATGGGGACCCAGAGTGGGGGCCCTCCCTGGCCCTGCTGGTGGTACTGAGCCCCCCAGC CCTAGACCAGCAGCTGTTTGCCCTGGCCCGGGTGCTGTCCCTGACTCTGAGGGTAGGACTCTGGGTAAGG AAGGATCGTGATGGCAGGGACATGGTGTACCCCTATCCTGGGGCCCGGGCTGAAGAAAAGCTAGGAGGAA CTCGGGACCCCACCTATCAGGAGAGAGCAGCCCCTCAAACGCAGCCCCTGGGCAAGGAGACCGACTCCCT CAGTGCTGGGTTTGTGGTGGTCATGGGTGTGGATTTGTCCCGCTGTGGCCCTGACCACCCGGCATCCCGC TGTCCCTGGGACCCTGGGCTTCTACTCCGCTTCCTTGCTGCGATGGCTGCAGTGGGAGCCCTGGAGCCCC TGCTGCCTGGACCACTGCTGGCTGTCCACCCTCATGCAGGGACCGCACCCCCTGCCAACCAGCTTCCCTG GCCTGTGCTGTGCTCCCCAGTGGCCGGGGTGATTCTCCTGGCCCTAGGGGCTCTTCTCGTCCTCCAGCTC ATCCGGCGTCGACGCCGAGAGCATGGAGCTCTCTGGCTGCCCCCTGGTTTCACTCGACGGCCTCGGACTC AGTCAGCTCCCCACCGACGCCGGCCCCCACTAGGCGAGGACAGCATTGGTCTCAAGGCACTGAAGCCAAA GGCAGAAGTTGATGAGGATGGAGTTGTGATGTGCTCAGGCCCTGAGGAGGGAGAGGAGGTGGGCCAGGCT GAAGAAACAGGCCCACCCTCCACGTGCCAGCTCTGGTCTCTGAGTGGTGGCTGTGGGGCGCTCCCTCAGG CAGCCATGCTAACTCCTCCCCAGGAATCTGAGATGGAAGCCCCTGACCTGGACACCCGTGGACCTGATGG GGTGACACCCCTGATGTCAGCAGTTTGCTGTGGGGAAGTACAGTCCGGGACCTTCCAAGGGGCATGGTTG GGATGTCCTGAGCCCTGGGAACCTCTGCTGGATGGAGGGGCCTGTCCCCAGGCTCACACCGTGGGCACTG GGGAGACCCCCCTGCACCTGGCTGCCCGATTCTCCCGGCCAACCGCTGCCCGCCGCCTCCTTGAGGCTGG AGCCAACCCCAACCAGCCAGACCGGGCAGGGCGCACACCCCTTCATGCTGCTGTGGCTGCTGATGCTCGG GAGGTCTGCCAGCTTCTGCTCCGTAGCAGACAAACTGCAGTGGACGCTCGCACAGAGGACGGGACCACAC CCTTGATGCTGGCTGCCAGGCTGGCGGTGGAAGACCTGGTTGAAGAACTGATTGCAGCCCAAGCAGACGT GGGGGCCAGAGATAAATGGGGGAAAACTGCGCTGCACTGGGCTGCTGCCGTGAACAACGCCCGAGCCGCC CGCTCGCTTCTCCAGGCCGGAGCCGATAAAGATGCCCAGGACAACAGGGAGCAGACGCCGCTATTCCTGG CGGCGCGGGAAGGAGCGGTGGAAGTAGCCCAGCTACTGCTGGGGCTGGGGGCAGCCCGAGAGCTGCGGGA CCAGGCTGGGCTAGCGCCGGCGGACGTCGCTCACCAACGTAACCACTGGGATCTGCTGACGCTGCTGGAA GGGGCTGGGCCACCAGAGGCCCGTCACAAAGCCACGCCGGGCCGCGAGGCTGGGCCCTTCCCGCGCGCAC GGACGGTGTCAGTAAGCGTGCCCCCGCATGGGGGCGGGGCTCTGCCGCGCTGCCGGACGCTGTCAGCCGG AGCAGGCCCTCGTGGGGGCGGAGCTTGTCTGCAGGCTCGGACTTGGTCCGTAGACTTGGCTGCGCGGGGG GGCGGGGCCTATTCTCATTGCCGGAGCCTCTCGGGAGTAGGAGCAGGAGGAGGCCCGACCCCTCGCGGCC GTAGGTTTTCTGCAGGCATGCGCGGGCCTCGGCCCAACCCTGCGATAATGCGAGGAAGATACGGAGTGGC TGCCGGGCGCGGAGGCAGGGTCTCAACGGATGACTGGCCCTGTGATTGGGTGGCCCTGGGAGCTTGCGGT TCTGCCTCCAACATTCCGATCCCGCCTCCTTGCCTTACTCCGTCCCCGGAGCGGGGATCACCTCAACTTG ACTGTGGTCCCCCAGCCCTCCAAGAAATGCCCATAAACCAAGGAGGAGAGGGTAAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221762 representing NM_004557
Red=Cloning site Green=Tags(s) MQPPSLLLLLLLLLLLCVSVVRPRGLLCGSFPEPCANGGTCLSLSLGQGTCQCAPGFLGETCQFPDPCQN AQLCQNGGSCQALLPAPLGLPSSPSPLTPSFLCTCLPGFTGERCQAKLEDPCPPSFCSKRGRCHIQASGR PQCSCMPGWTGEQCQLRDFCSANPCVNGGVCLATYPQIQCHCPPGFEGHACERDVNECFQDPGPCPKGTS CHNTLGSFQCLCPVGQEGPRCELRAGPCPPRGCSNGGTCQLMPEKDSTFHLCLCPPGFIGPDCEVNPDNC VSHQCQNGGTCQDGLDTYTCLCPETWTGWDCSEDVDECETQGPPHCRNGGTCQNSAGSFHCVCVSGWGGT SCEENLDDCIAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHGDAQCSTNPLTGSTLCLC QPGYSGPTCHQDLDECLMAQQGPSPCEHGGSCLNTPGSFNCLCPPGYTGSRCEADHNECLSQPCHPGSTC LDLLATFHCLCPPGLEGQLCEVETNECASAPCLNHADCHDLLNGFQCICLPGFSGTRCEEDIDECRSSPC ANGGQCQDQPGAFHCKCLPGFEGPRCQTEVDECLSDPCPVGASCLDLPGAFFCLCPSGFTGQLCEVPLCA PNLCQPKQICKDQKDKANCLCPDGSPGCAPPEDNCTCHHGHCQRSSCVCDVGWTGPECEAELGGCISAPC AHGGTCYPQPSGYNCTCPTGYTGPTCSEEMTACHSGPCLNGGSCNPSPGGYYCTCPPSHTGPQCQTSTDY CVSAPCFNGGTCVNRPGTFSCLCAMGFQGPRCEGKLRPSCADSPCRNRATCQDSPQGPRCLCPTGYTGGS CQTLMDLCAQKPCPRNSHCLQTGPSFHCLCLQGWTGPLCNLPLSSCQKAALSQGIDVSSLCHNGGLCVDS GPSYFCHCPPGFQGSLCQDHVNPCESRPCQNGATCMAQPSGYLCQCAPGYDGQNCSKELDACQSQPCHNH GTCTPKPGGFHCACPPGFVGLRCEGDVDECLDQPCHPTGTAACHSLANAFYCQCLPGHTGQWCEVEIDPC HSQPCFHGGTCEATAGSPLGFICHCPKGFEGPTCSHRAPSCGFHHCHHGGLCLPSPKPGFPPRCACLSGY GGPDCLTPPAPKGCGPPSPCLYNGSCSETTGLGGPGFRCSCPHSSPGPRCQKPGAKGCEGRSGDGACDAG CSGPGGNWDGGDCSLGVPDPWKGCPSHSRCWLLFRDGQCHPQCDSEECLFDGYDCETPPACTPAYDQYCH DHFHNGHCEKGCNTAECGWDGGDCRPEDGDPEWGPSLALLVVLSPPALDQQLFALARVLSLTLRVGLWVR KDRDGRDMVYPYPGARAEEKLGGTRDPTYQERAAPQTQPLGKETDSLSAGFVVVMGVDLSRCGPDHPASR CPWDPGLLLRFLAAMAAVGALEPLLPGPLLAVHPHAGTAPPANQLPWPVLCSPVAGVILLALGALLVLQL IRRRRREHGALWLPPGFTRRPRTQSAPHRRRPPLGEDSIGLKALKPKAEVDEDGVVMCSGPEEGEEVGQA EETGPPSTCQLWSLSGGCGALPQAAMLTPPQESEMEAPDLDTRGPDGVTPLMSAVCCGEVQSGTFQGAWL GCPEPWEPLLDGGACPQAHTVGTGETPLHLAARFSRPTAARRLLEAGANPNQPDRAGRTPLHAAVAADAR EVCQLLLRSRQTAVDARTEDGTTPLMLAARLAVEDLVEELIAAQADVGARDKWGKTALHWAAAVNNARAA RSLLQAGADKDAQDNREQTPLFLAAREGAVEVAQLLLGLGAARELRDQAGLAPADVAHQRNHWDLLTLLE GAGPPEARHKATPGREAGPFPRARTVSVSVPPHGGGALPRCRTLSAGAGPRGGGACLQARTWSVDLAARG GGAYSHCRSLSGVGAGGGPTPRGRRFSAGMRGPRPNPAIMRGRYGVAAGRGGRVSTDDWPCDWVALGACG SASNIPIPPPCLTPSPERGSPQLDCGPPALQEMPINQGGEGKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004557 |
| ORF Size | 6009 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004557.4 |
| RefSeq Size | 6762 bp |
| RefSeq ORF | 6012 bp |
| Locus ID | 4855 |
| UniProt ID | Q99466 |
| Protein Families | Druggable Genome |
| Protein Pathways | Dorso-ventral axis formation, Notch signaling pathway |
| MW | 210.1 kDa |
| Gene Summary | This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221762L1 | Lenti ORF clone of Human notch 4 (NOTCH4), Myc-DDK-tagged |
CNY 22,232.00 |
|
| RG221762 | NOTCH4 (tGFP-tagged) - Human notch 4 (NOTCH4) |
CNY 21,432.00 |
|
| SC303498 | NOTCH4 (untagged)-Human notch 4 (NOTCH4) |
CNY 13,232.00 |
