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NEDD4 (NM_006154) Human Tagged ORF Clone
CAT＃: RC221706
NEDD4 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1
ORF Plasmid: tGFP
"NM_006154" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NEDD4-1; RPF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221706 representing NM_006154
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGAGGACGAGGAAAATTCACGAATTGTGAGAGTAA GAGTTATAGCCGGAATAGGCCTTGCCAAGAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGAC GTTATATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAAAAAGAGTTTGAATCCA AAGTGGAATGAAGAAATATTATTCAGAGTTCATCCTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACG AAAACCGATTGACAAGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGACAGAAAA TCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTCATCCAAGAAGTCACAAATCAAGAGTT AAAGGTTATCTGAGACTAAAAATGACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAAC AGGCTGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTTGCCATTTGCAGCAACA ACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAAGAGAGGCAGGATATCCTTGGAAGGACCTATTATGTA AACCATGAATCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTGAGAATG GCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATATCCGAGGAAACAGAAAGTGT TGACAACCAAGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAGCCAG GCCTTCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCAGAAGAATTGAATGCCA GACTCACCATTTTTGGAAATTCAGCCGTGAGCCAGCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGG CAGCTTACAAGCCTATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCTGGATTA CCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTAGATCACAATTCCAGAACGA CTACTTGGACAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGC AGGCCCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCCATCTGAAATTGAGCAA GGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATGCACCAAATGGGAGGCCTTTCTTTATTGACCACAACA CTAAAACCACCACCTGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATCACTTGA TACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGAGAACTCACACAGATGGAAGAATCTTC TACATAAATCACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGAC CAGCAGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAAAGTTGAAGAAGCAGAA TGACATTCCAAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATT ATGGGTGTCAAGAGAGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGGGATTGG ATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAGGAAATGTTTAACCCTTATTATGGGTT GTTTGAATATTCTGCTACGGACAATTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGAT CACCTCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCATGGCAAACTGTTGGATG GTTTTTTCATCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCCTTCATGATATGGAATCTGT GGATAGTGAATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTCAGGTTT ATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCTGAAAAATGGTGGATCAGAAATAGTTG TCACCAATAAGAACAAAAAGGAATATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAA GCAAATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCATCAAAATTTTTGATGAA AATGAACTAGAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGACTGGAGGGAACATACAAAGT ATAAAAATGGCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGATGGATTC AGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGTGCCTATGAATGGATTTGCTGAA CTATACGGTTCAAATGGACCACAGTCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAG CTCATACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAATTATGGGATAAACTTCA GATGGCAATTGAAAACACCCAGGGCTTTGATGGAGTTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221706 representing NM_006154
Red=Cloning site Green=Tags(s) MATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNP KWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRV KGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYV NHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNQESSENWEIIREDEATMYSSQ AFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGL PPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQ GFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIF YINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRI MGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNED HLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRF IIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDE NELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAE LYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006154 |
| ORF Size | 2700 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006154.1, NP_006145.1 |
| RefSeq Size | 5749 bp |
| RefSeq ORF | 2703 bp |
| Locus ID | 4734 |
| UniProt ID | P46934 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Ubiquitin mediated proteolysis |
| MW | 104 kDa |
| Gene Summary | This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221706L1 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221706L2 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC221706L3 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221706L4 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG221706 | NEDD4 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1 |
CNY 4,370.00 |
|
| SC310054 | NEDD4 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1 |
CNY 6,600.00 |
