Semaphorin 5A (SEMA5A) (NM_003966) Human Tagged ORF Clone

CAT＃: RC221685

SEMA5A (Myc-DDK-tagged)-Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A)

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_003966" in other vectors (4)

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CNY 7,480.00

货期*
现货
规格
    • 10 ug

Product images

经常一起买 (5)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA311279
Goat Anti-SEMA5A (Semaphorin 5A) Antibody
    • 100 ug

CNY 5,371.00

添加到购物车

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms SEMAF; semF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC221685 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGGAACCTGTGTTATAGCATGGCTGTTCTCAAGCCTGGGGCTGTGGAGACTCGCCCACCCAGAGG
CCCAGGGTACGACTCAGTGCCAGAGAACCGAGCATCCAGTCATCTCCTATAAAGAAATTGGCCCCTGGTT
ACGGGAGTTCAGAGCGAAGAATGCTGTGGATTTCTCGCAGTTAACATTTGACCCAGGACAGAAAGAACTT
GTTGTAGGAGCAAGAAACTACCTCTTCAGGTTACAGCTTGAGGATCTGTCTCTTATCCAGGCTGTGGAAT
GGGAGTGTGATGAAGCTACCAAAAAGGCCTGTTACAGCAAAGGCAAATCAAAGGAGGAATGTCAGAACTA
CATCCGGGTGCTTCTGGTGGGTGGCGACCGGTTATTCACCTGTGGGACCAATGCATTCACGCCTGTCTGC
ACCAACCGCTCGTTGAGCAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCCCGCTGTCCCTACA
GTCCCCAGCACAATTCCACAGCGCTCCTCACAGCTGGTGGGGAGCTCTATGCTGCTACAGCCATGGATTT
TCCAGGACGTGATCCTGCCATTTACCGAAGCCTAGGCATTTTACCTCCTCTCCGCACGGCGCAGTACAAC
TCCAAATGGCTCAATGAGCCAAACTTTGTGTCATCTTATGACATCGGAAATTTTACCTACTTCTTTTTCC
GAGAAAATGCAGTAGAGCATGACTGTGGGAAAACAGTGTTCTCCAGAGCTGCCCGGGTGTGCAAGAACGA
TATTGGTGGGCGCTTCCTGCTGGAAGACACCTGGACCACATTCATGAAGGCTCGCCTGAACTGCTCCCGT
CCTGGGGAAGTCCCCTTTTACTACAACGAATTGCAGAGTACTTTCTTCCTGCCTGAGCTGGATTTGATCT
ATGGCATCTTTACCACCAATGTGAACAGCATTGCGGCCTCAGCTGTGTGCGTCTTCAACCTGAGCGCCAT
CGCGCAGGCCTTCTCTGGGCCCTTCAAGTACCAAGAAAACTCGCGCTCGGCCTGGCTACCGTATCCCAAC
CCAAACCCCCACTTCCAGTGTGGCACCGTGGACCAGGGCCTGTACGTGAACCTGACCGAGAGAAATCTGC
AGGATGCTCAGAAGTTCATTCTGATGCATGAGGTGGTACAGCCAGTGACCACAGTGCCCTCCTTCATGGA
GGACAATAGCCGCTTTTCCCACGTGGCAGTCGACGTGGTGCAGGGCAGAGAAGCGCTCGTCCACATCATC
TATTTGGCCACAGATTACGGAACCATTAAGAAAGTGCGGGTACCCCTGAATCAGACCTCAAGCAGCTGTT
TGCTGGAAGAGATTGAGCTCTTCCCTGAGAGGCGGAGGGAGCCCATCAGGAGCCTGCAGATCCTGCACAG
CCAGAGTGTCCTGTTCGTGGGCCTGCGGGAGCACGTGGTCAAGATCCCCCTGAAGAGGTGCCAGTTCTAC
CGCACACGCAGCACCTGCATTGGGGCCCAGGACCCTTACTGTGGCTGGGATGTGGTAATGAAGAAATGCA
CAAGCCTGGAGGAGAGCCTGAGCATGACGCAGTGGGAACAGAGCATCTCTGCGTGTCCGACCAGGAATCT
CACCGTGGATGGGCACTTTGGTGTGTGGTCTCCGTGGACGCCTTGCACGCACACAGATGGCAGCGCCGTG
GGATCCTGCCTCTGTCGAACCCGCTCCTGCGACAGCCCGGCCCCGCAGTGTGGTGGCTGGCAGTGCGAGG
GCCCTGGCATGGAGATCGCCAACTGTTCCAGGAACGGAGGCTGGACTCCCTGGACCTCGTGGTCTCCCTG
CAGCACTACCTGTGGGATCGGCTTCCAGGTGCGGCAGCGCTCCTGCAGCAACCCCACTCCCAGGCACGGG
GGCCGGGTGTGCGTGGGACAGAACCGCGAGGAAAGATACTGCAATGAACATTTGCTATGTCCCCCACACA
TGTTCTGGACAGGCTGGGGTCCTTGGGAACGGTGCACAGCCCAATGCGGGGGTGGCATTCAAGCTCGCCG
CAGGATCTGTGAGAATGGGCCTGACTGTGCAGGCTGCAATGTGGAGTACCAGTCTTGCAACACCAACCCG
TGTCCTGAGCTGAAGAAGACCACGCCCTGGACACCCTGGACACCTGTCAACATCTCTGACAACGGCGGCC
ACTATGAGCAACGATTCCGATACACATGCAAAGCCCGCCTGGCTGATCCGAATTTGCTGGAAGTGGGAAG
ACAGAGAATCGAAATGCGGTACTGTTCTAGCGACGGCACCAGTGGCTGCTCCACAGATGGGCTTTCTGGG
GATTTCCTGCGTGCTGGGAGATACTCTGCCCACACGGTCAACGGGGCTTGGTCAGCCTGGACGTCGTGGT
CACAGTGCAGCCGTGACTGCAGCAGGGGCATTCGGAACCGGAAGCGTGTTTGCAACAACCCCGAACCCAA
GTATGGGGGAATGCCTTGCCTTGGCCCATCTCTGGAATACCAGGAATGCAACATTTTGCCCTGCCCAGTG
GATGGCGTGTGGTCTTGCTGGTCCCCCTGGACAAAATGTTCAGCAACATGCGGCGGTGGACACTATATGA
GGACCCGCTCTTGCTCCAATCCAGCCCCGGCCTATGGAGGGGACATCTGCCTGGGGCTGCACACAGAAGA
GGCACTCTGCAACACGCAGCCCTGCCCAGAGAGCTGGTCGGAGTGGTCGGACTGGTCTGAGTGTGAAGCC
TCTGGCGTCCAAGTCCGCGCCCGCCAGTGCATCCTCCTGTTCCCCATGGGCAGCCAGTGCTCCGGGAACA
CCACGGAGAGCCGGCCGTGTGTGTTTGACTCTAATTTCATCCCAGAAGTATCTGTGGCAAGATCCAGTAG
CGTAGAAGAGAAAAGGTGTGGAGAGTTCAACATGTTCCACATGATCGCCGTGGGGCTGAGCAGCTCCATC
CTCGGCTGCCTCCTCACCCTGCTCGTCTATACTTACTGCCAGCGGTACCAGCAGCAATCCCACGATGCGA
CTGTCATCCACCCCGTCTCACCTGCCCCCCTTAATACCAGCATAACCAACCACATCAACAAACTGGACAA
GTACGACTCGGTGGAGGCCATCAAGGCATTTAACAAAAACAACTTGATCCTAGAGGAAAGAAACAAATAC
TTCAACCCACATCTCACTGGGAAGACCTATTCTAATGCCTACTTTACAGATCTCAATAATTATGATGAGT
AC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC221685 protein sequence
Red=Cloning site Green=Tags(s)

MKGTCVIAWLFSSLGLWRLAHPEAQGTTQCQRTEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKEL
VVGARNYLFRLQLEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVC
TNRSLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYN
SKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSR
PGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAIAQAFSGPFKYQENSRSAWLPYPN
PNPHFQCGTVDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHVAVDVVQGREALVHII
YLATDYGTIKKVRVPLNQTSSSCLLEEIELFPERRREPIRSLQILHSQSVLFVGLREHVVKIPLKRCQFY
RTRSTCIGAQDPYCGWDVVMKKCTSLEESLSMTQWEQSISACPTRNLTVDGHFGVWSPWTPCTHTDGSAV
GSCLCRTRSCDSPAPQCGGWQCEGPGMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHG
GRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQARRRICENGPDCAGCNVEYQSCNTNP
CPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLADPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSG
DFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKYGGMPCLGPSLEYQECNILPCPV
DGVWSCWSPWTKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSDWSECEA
SGVQVRARQCILLFPMGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMIAVGLSSSI
LGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKY
FNPHLTGKTYSNAYFTDLNNYDEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003966
ORF Size 3222 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003966.3
RefSeq Size 11825 bp
RefSeq ORF 3225 bp
Locus ID 9037
UniProt ID Q13591
Protein Families Transmembrane
Protein Pathways Axon guidance
MW 120.6 kDa
Gene Summary This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RC221685L3 Lenti ORF clone of Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), Myc-DDK-tagged
    • 10 ug

CNY 7,600.00

RC221685L4 Lenti ORF clone of Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), mGFP tagged
    • 10 ug

CNY 7,600.00

RG221685 SEMA5A (tGFP-tagged) - Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A)
    • 10 ug

CNY 6,270.00

SC303407 SEMA5A (untagged)-Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A)
    • 10 ug

CNY 7,488.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

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RC221685L3V

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CNY 6,270.00

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