Neurotensin Receptor 1 (NTSR1) (NM_002531) Human Tagged ORF Clone

CAT#: RC220548

NTSR1 (Myc-DDK-tagged)-Human neurotensin receptor 1 (high affinity) (NTSR1)

ORF Plasmid: DDK tGFP



  "NM_002531" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-NTSR1 Antibody (Cytoplasmic Domain)
    • 50 ug

CNY 5,654.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms NTR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220548 representing NM_002531
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCTCAACAGCTCCGCGCCGGGAACCCCGGGCACGCCGGCCGCCGACCCCTTCCAGCGGGCGCAGG
CCGGACTGGAGGAGGCGCTGCTGGCCCCGGGCTTCGGCAACGCTTCGGGCAACGCGTCGGAGCGCGTCCT
GGCGGCACCCAGCAGCGAGCTGGACGTGAACACCGACATCTACTCCAAAGTGCTGGTGACCGCCGTGTAC
CTGGCGCTCTTCGTGGTGGGCACGGTGGGCAACACGGTGACGGCGTTCACGCTGGCGCGGAAGAAGTCGC
TGCAGAGCCTGCAGAGCACGGTGCATTACCACCTGGGCAGCCTGGCGCTGTCCGACCTGCTCACCCTGCT
GCTGGCCATGCCCGTGGAGCTGTACAACTTCATCTGGGTGCACCACCCCTGGGCCTTCGGCGACGCCGGC
TGCCGCGGCTACTACTTCCTGCGCGACGCCTGCACCTACGCCACGGCCCTCAACGTGGCCAGCCTGAGTG
TGGAGCGCTACCTGGCCATCTGCCACCCCTTCAAGGCCAAGACCCTCATGTCCCGAAGCCGCACCAAGAA
GTTCATCAGCGCCATCTGGCTCGCCTCGGCCCTGCTGACGGTGCCTATGCTGTTCACCATGGGCGAGCAG
AACCGCAGCGCCGACGGCCAGCACGCCGGCGGCCTGGTGTGCACCCCCACCATCCACACTGCCACCGTCA
AGGTCGTCATACAGGTCAACACCTTCATGTCCTTCATATTCCCCATGGTGGTCATCTCGGTCCTGAACAC
CATCATCGCCAACAAGCTGACCGTCATGGTACGCCAGGCGGCCGAGCAGGGCCAAGTGTGCACGGTCGGG
GGCGAGCACAGCACATTCAGCATGGCCATCGAGCCTGGCAGGGTCCAGGCCCTGCGGCACGGCGTGCGCG
TCCTACGTGCAGTGGTCATCGCCTTTGTGGTCTGCTGGCTGCCCTACCACGTGCGGCGCCTCATGTTCTG
CTACATCTCGGATGAGCAGTGGACTCCGTTCCTCTATGACTTCTACCACTACTTCTACATGGTGACCAAC
GCACTCTTCTACGTCAGCTCCACCATCAACCCCATCCTGTACAACCTCGTCTCTGCCAACTTCCGCCACA
TCTTCCTGGCCACACTGGCCTGCCTCTGCCCGGTGTGGCGGCGCAGGAGGAAGAGGCCAGCCTTCTCGAG
GAAGGCCGACAGCGTGTCCAGCAACCACACCCTCTCCAGCAATGCCACCCGCGAGACGCTGTAC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC220548 representing NM_002531
Red=Cloning site Green=Tags(s)

MRLNSSAPGTPGTPAADPFQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDIYSKVLVTAVY
LALFVVGTVGNTVTAFTLARKKSLQSLQSTVHYHLGSLALSDLLTLLLAMPVELYNFIWVHHPWAFGDAG
CRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLTVPMLFTMGEQ
NRSADGQHAGGLVCTPTIHTATVKVVIQVNTFMSFIFPMVVISVLNTIIANKLTVMVRQAAEQGQVCTVG
GEHSTFSMAIEPGRVQALRHGVRVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTPFLYDFYHYFYMVTN
ALFYVSSTINPILYNLVSANFRHIFLATLACLCPVWRRRRKRPAFSRKADSVSSNHTLSSNATRETLY

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002531
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002531.1, NP_002522.1
RefSeq Size 4131 bp
RefSeq ORF 1257 bp
Locus ID 4923
UniProt ID P30989
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction
MW 46.1 kDa
Gene Summary Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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