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JAK2 (NM_004972) Human Tagged ORF Clone
CAT＃: RC220503
- TrueORF®
JAK2 (Myc-DDK-tagged)-Human Janus kinase 2 (JAK2)
ORF Plasmid: tGFP
"NM_004972" in other vectors (6)
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CNY 11,824.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JTK10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220503 representing NM_004972
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAATGGCCTGCCTTACGATGACAGAAATGGAGGGAACATCCACCTCTTCTATATATCAGAATGGTG ATATTTCTGGAAATGCCAATTCTATGAAGCAAATAGATCCAGTTCTTCAGGTGTATCTTTACCATTCCCT TGGGAAATCTGAGGCAGATTATCTGACCTTTCCATCTGGGGAGTATGTTGCAGAAGAAATCTGTATTGCT GCTTCTAAAGCTTGTGGTATCACACCTGTGTATCATAATATGTTTGCTTTAATGAGTGAAACAGAAAGGA TCTGGTATCCACCCAACCATGTCTTCCATATAGATGAGTCAACCAGGCATAATGTACTCTACAGAATAAG ATTTTACTTTCCTCGTTGGTATTGCAGTGGCAGCAACAGAGCCTATCGGCATGGAATATCTCGAGGTGCT GAAGCTCCTCTTCTTGATGACTTTGTCATGTCTTACCTCTTTGCTCAGTGGCGGCATGATTTTGTGCACG GATGGATAAAAGTACCTGTGACTCATGAAACACAGGAAGAATGTCTTGGGATGGCAGTGTTAGATATGAT GAGAATAGCCAAAGAAAACGATCAAACCCCACTGGCCATCTATAACTCTATCAGCTACAAGACATTCTTA CCAAAATGTATTCGAGCAAAGATCCAAGACTATCATATTTTGACAAGGAAGCGAATAAGGTACAGATTTC GCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCCAGAAACTTGAAACTTAAGTATCTTATAAA TCTGGAAACTCTGCAGTCTGCCTTCTACACAGAGAAATTTGAAGTAAAAGAACCTGGAAGTGGTCCTTCA GGTGAGGAGATTTTTGCAACCATTATAATAACTGGAAACGGTGGAATTCAGTGGTCAAGAGGGAAACATA AAGAAAGTGAGACACTGACAGAACAGGATTTACAGTTATATTGCGATTTTCCTAATATTATTGATGTCAG TATTAAGCAAGCAAACCAAGAGGGTTCAAATGAAAGCCGAGTTGTAACTATCCATAAGCAAGATGGTAAA AATCTGGAAATTGAACTTAGCTCATTAAGGGAAGCTTTGTCTTTCGTGTCATTAATTGATGGATATTATA GATTAACTGCAGATGCACATCATTACCTCTGTAAAGAAGTAGCACCTCCAGCCGTGCTTGAAAATATACA AAGCAACTGTCATGGCCCAATTTCGATGGATTTTGCCATTAGTAAACTGAAGAAAGCAGGTAATCAGACT GGACTGTATGTACTTCGATGCAGTCCTAAGGACTTTAATAAATATTTTTTGACTTTTGCTGTCGAGCGAG AAAATGTCATTGAATATAAACACTGTTTGATTACAAAAAATGAGAATGAAGAGTACAACCTCAGTGGGAC AAAGAAGAACTTCAGCAGTCTTAAAGATCTTTTGAATTGTTACCAGATGGAAACTGTTCGCTCAGACAAT ATAATTTTCCAGTTTACTAAATGCTGTCCCCCAAAGCCAAAAGATAAATCAAACCTTCTAGTCTTCAGAA CGAATGGTGTTTCTGATGTACCAACCTCACCAACATTACAGAGGCCTACTCATATGAACCAAATGGTGTT TCACAAAATCAGAAATGAAGATTTGATATTTAATGAAAGCCTTGGCCAAGGCACTTTTACAAAGATTTTT AAAGGCGTACGAAGAGAAGTAGGAGACTACGGTCAACTGCATGAAACAGAAGTTCTTTTAAAAGTTCTGG ATAAAGCACACAGAAACTATTCAGAGTCTTTCTTTGAAGCAGCAAGTATGATGAGCAAGCTTTCTCACAA GCATTTGGTTTTAAATTATGGAGTATGTGTCTGTGGAGACGAGAATATTCTGGTTCAGGAGTTTGTAAAA TTTGGATCACTAGATACATATCTGAAAAAGAATAAAAATTGTATAAATATATTATGGAAACTTGAAGTTG CTAAACAGTTGGCATGGGCCATGCATTTTCTAGAAGAAAACACCCTTATTCATGGGAATGTATGTGCCAA AAATATTCTGCTTATCAGAGAAGAAGACAGGAAGACAGGAAATCCTCCTTTCATCAAACTTAGTGATCCT GGCATTAGTATTACAGTTTTGCCAAAGGACATTCTTCAGGAGAGAATACCATGGGTACCACCTGAATGCA TTGAAAATCCTAAAAATTTAAATTTGGCAACAGACAAATGGAGTTTTGGTACCACTTTGTGGGAAATCTG CAGTGGAGGAGATAAACCTCTAAGTGCTCTGGATTCTCAAAGAAAGCTACAATTTTATGAAGATAGGCAT CAGCTTCCTGCACCAAAGTGGGCAGAATTAGCAAACCTTATAAATAATTGTATGGATTATGAACCAGATT TCAGGCCTTCTTTCAGAGCCATCATACGAGATCTTAACAGTTTGTTTACTCCAGATTATGAACTATTAAC AGAAAATGACATGTTACCAAATATGAGGATAGGTGCCCTGGGGTTTTCTGGTGCCTTTGAAGACCGGGAT CCTACACAGTTTGAAGAGAGACATTTGAAATTTCTACAGCAACTTGGCAAGGGTAATTTTGGGAGTGTGG AGATGTGCCGGTATGACCCTCTACAGGACAACACTGGGGAGGTGGTCGCTGTAAAAAAGCTTCAGCATAG TACTGAAGAGCACCTAAGAGACTTTGAAAGGGAAATTGAAATCCTGAAATCCCTACAGCATGACAACATT GTAAAGTACAAGGGAGTGTGCTACAGTGCTGGTCGGCGTAATCTAAAATTAATTATGGAATATTTACCAT ATGGAAGTTTACGAGACTATCTTCAAAAACATAAAGAACGGATAGATCACATAAAACTTCTGCAGTACAC ATCTCAGATATGCAAGGGTATGGAGTATCTTGGTACAAAAAGGTATATCCACAGGGATCTGGCAACGAGA AATATATTGGTGGAGAACGAGAACAGAGTTAAAATTGGAGATTTTGGGTTAACCAAAGTCTTGCCACAAG ACAAAGAATACTATAAAGTAAAAGAACCTGGTGAAAGTCCCATATTCTGGTATGCTCCAGAATCACTGAC AGAGAGCAAGTTTTCTGTGGCCTCAGATGTTTGGAGCTTTGGAGTGGTTCTGTATGAACTTTTCACATAC ATTGAGAAGAGTAAAAGTCCACCAGCGGAATTTATGCGTATGATTGGCAATGACAAACAAGGACAGATGA TCGTGTTCCATTTGATAGAACTTTTGAAGAATAATGGAAGATTACCAAGACCAGATGGATGCCCAGATGA GATCTATATGATCATGACAGAATGCTGGAACAATAATGTAAATCAACGCCCCTCCTTTAGGGATCTAGCT CTTCGAGTGGATCAAATAAGGGATAACATGGCTGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220503 representing NM_004972
Red=Cloning site Green=Tags(s) MGMACLTMTEMEGTSTSSIYQNGDISGNANSMKQIDPVLQVYLYHSLGKSEADYLTFPSGEYVAEEICIA ASKACGITPVYHNMFALMSETERIWYPPNHVFHIDESTRHNVLYRIRFYFPRWYCSGSNRAYRHGISRGA EAPLLDDFVMSYLFAQWRHDFVHGWIKVPVTHETQEECLGMAVLDMMRIAKENDQTPLAIYNSISYKTFL PKCIRAKIQDYHILTRKRIRYRFRRFIQQFSQCKATARNLKLKYLINLETLQSAFYTEKFEVKEPGSGPS GEEIFATIIITGNGGIQWSRGKHKESETLTEQDLQLYCDFPNIIDVSIKQANQEGSNESRVVTIHKQDGK NLEIELSSLREALSFVSLIDGYYRLTADAHHYLCKEVAPPAVLENIQSNCHGPISMDFAISKLKKAGNQT GLYVLRCSPKDFNKYFLTFAVERENVIEYKHCLITKNENEEYNLSGTKKNFSSLKDLLNCYQMETVRSDN IIFQFTKCCPPKPKDKSNLLVFRTNGVSDVPTSPTLQRPTHMNQMVFHKIRNEDLIFNESLGQGTFTKIF KGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVK FGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDP GISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRH QLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRD PTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNI VKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATR NILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTY IEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLA LRVDQIRDNMAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004972 |
| ORF Size | 3396 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004972.4 |
| RefSeq Size | 5097 bp |
| RefSeq ORF | 3399 bp |
| Locus ID | 3717 |
| UniProt ID | O60674 |
| Domains | B41, pkinase, SH2, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway |
| MW | 130.5 kDa |
| Gene Summary | This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
JAK1 Pseudokinase V666G Mutant Dominantly Impairs JAK3 Phosphorylation and IL-2 Signaling.
,null,
International journal of molecular sciences
,PubMed ID 37047778
[JAK2]
|
|
Apatinib weakens resistance of gastric cancer cells to paclitaxel by suppressing JAK/STAT3 signaling pathway.
,null,
Drug development research
,PubMed ID 34405891
[JAK2]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220503L1 | Lenti ORF clone of Human Janus kinase 2 (JAK2), Myc-DDK-tagged |
CNY 13,210.00 |
|
| RC220503L2 | Lenti ORF clone of Human Janus kinase 2 (JAK2), mGFP tagged |
CNY 14,224.00 |
|
| RC220503L3 | Lenti ORF clone of Human Janus kinase 2 (JAK2), Myc-DDK-tagged |
CNY 14,224.00 |
|
| RC220503L4 | Lenti ORF clone of Human Janus kinase 2 (JAK2), mGFP tagged |
CNY 13,210.00 |
|
| RG220503 | JAK2 (tGFP-tagged) - Human Janus kinase 2 (JAK2) |
CNY 13,424.00 |
|
| SC316317 | JAK2 (untagged)-Human Janus kinase 2 (JAK2) |
CNY 7,888.00 |
