Myosin (MYH4) (NM_017533) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MYH2B; MyHC-2B; MyHC-IIb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC220101 representing NM_017533 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTGACTCTGAGATGGCCATTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAAGGAGC GAATTGAAGCTCAGAACAAGCCTTTTGATGCCAAGACATCAGTCTTTGTGGTGGACCCTAAGGAGTCCTA CGTGAAAGCAATAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCCAAGACCGAAGCTGGAGCTACTGTA ACTGTGAAAGAAGACCAAGTCTTCTCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGGCCATGA TGACTCACCTGCATGAGCCTGCTGTGCTGTATAACCTCAAAGAGCGTTACGCAGCCTGGATGATCTACAC CTACTCGGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAGGTGGTG ACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCATATCTTCTCCATCTCTGACAATGCCTATC AGTTCATGCTAACTGATCGTGAAAACCAGTCAATCTTGATTACTGGAGAATCTGGTGCAGGGAAGACTGT GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGAGAGAAGAAAAAAGAGGAACCT GCCTCTGGCAAAATGCAGGGGACCCTTGAAGATCAAATCATCAGTGCTAACCCCCTACTGGAAGCCTTCG GCAATGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATCCATTTTGGTGC CACAGGCAAACTGGCTTCTGCAGATATTGAAACATATCTGCTAGAGAAGTCCCGAGTTACTTTTCAGCTA AAGGCTGAAAGAAGCTACCACATATTTTATCAAATCCTGTCCAATAAGAAACCAGAGCTCATTGAAATGC TTCTGATCACCACCAACCCATATGACTTCGCATTTGTCAGCCAAGGGGAAATTACTGTGCCCAGCATTGA TGACCAGGAAGAGCTGATGGCCACAGATAGTGCTGTGGACATCCTGGGTTTCACTGCTGATGAAAAGGTG GCCATTTACAAGCTCACTGGAGCCGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAAAGGGAAG AGCAGGCAGAGCCAGATGGCACGGAAGTTGCTGACAAAGCTGCTTATCTGACAAGTCTGAACTCTGCTGA CCTGCTCAAATCTCTCTGCTATCCCAGAGTCAAGGTCGGCAATGAGTTCGTAACCAAAGGCCAGACTGTG CAGCAGGTGTACAATGCAGTGGGTGCTCTGGCCAAAGCCATCTACGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATTGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAAGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCA TTGACTTCGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCT AGAAGAGGAGTGCATGTTCCCCAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGAACAACATCTT GGAAAATCCAACAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGCCTGAGGCTCACTTCTCACTGGTGC ACTATGCCGGCACCGTGGACTACAACATCGCCGGCTGGCTGGACAAAAACAAGGACCCCCTGAATGAGAC TGTGGTGGGGCTGTACCAGAAGTCTGCAATGAAGACTCTGGCTTTCCTCTTCTCTGGGGCACAAACTGCT GAAGCAGAGGGTGGTGGTGGAAAGAAAGGTGGCAAAAAGAAGGGTTCTTCTTTCCAGACAGTGTCAGCTC TTTTCAGGGAGAATTTGAATAAGCTGATGACCAACTTGAGGAGCACTCACCCCCACTTTGTGCGGTGCAT CATCCCCAATGAAACTAAAACTCCTGGTGCCATGGAGCATGAGCTTGTCCTGCATCAGCTGAGGTGTAAC GGTGTGCTGGAAGGCATCCGCATCTGCAGGAAAGGCTTCCCAAGCAGAATCCTTTATGCAGACTTCAAAC AGAGATACAAGGTTCTAAATGCGAGTGCTATCCCAGAGGGTCAGTTCATTGACAGCAAGAAGGCTTCTGA GAAACTTCTAGGGTCTATTGAAATTGACCACACCCAGTACAAATTCGGTCATACCAAGGTTTTCTTCAAA GCTGGCCTGCTGGGAACTCTAGAGGAAATGCGAGATGAAAAGCTAGCTCAACTCATCACGCGCACTCAAG CCATATGCAGAGGGTTCCTGATGAGAGTGGAGTTCAGAAAGATGATGGAGAGGAGAGAGTCCATCTTCTG CATTCAGTACAACATCCGTGCTTTCATGAATGTGAAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATC AAGCCCCTCCTCAAGAGTGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAAGAATTTGAGAAAACCA AAGAAGAGCTGGCTAAGACAGAGGCAAAAAGGAAAGAACTAGAAGAAAAGATGGTGACGCTAATGCAAGA GAAAAATGACTTACAACTCCAAGTTCAAGCTGAAGCAGATGCCTTGGCTGATGCAGAGGAAAGATGTGAT CAGTTGATTAAAACCAAAATCCAACTTGAGGCCAAAATCAAAGAGGTAACTGAAAGAGCTGAGGATGAGG AAGAGATCAATGCTGAGCTGACAGCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAGCTCAAGAAAGA CATTGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAGAACAAGGTGAAA AACCTCACAGAAGAGATGGCAGGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGGCTCTCC AGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGATGGAGGAGGACAAAGTCAACACCCTGACCAAAGC TAAAACCAAGCTAGAACAGCAAGTGGACGATCTTGAAGGATCTCTGGAACAAGAAAAGAAACTTTGCATG GACTTAGAAAGAGCCAAGAGAAAACTGGAGGGTGACCTAAAATTGGCCCAAGAATCCACAATGGATACAG AAAATGACAAACAGCAACTTAATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAATCTGCAAGGCAA GATTGAAGATGAACAAGCCCTTGCAATACAGCTACAAAAGAAGATCAAAGAATTACAGGCCCGCATTGAG GAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTGACCTCT CCCGGGAGCTGGAGGAGATCAGTGAGAGGCTGGAAGAAGCCGGTGGGGCCACTTCAGCCCAGATTGAGAT GAACAAGAAGCGGGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAAGAGTCCACCCTGCAGCACGAA GCCACGGCAGCTGCTCTTCGGAAGAAGCACGCAGATAGTGTGGCTGAGCTTGGGGAGCAGATTGACAGCC TTCAGCGGGTCAAGCAGAAGCTGGAGAAGGAAAAGAGTGAGCTGAAGATGGAGATCAATGACCTTGCTAG TAACATGGAGACTGTCTCCAAAGCCAAGGCAAACTTTGAGAAAATGTGCCGCACCCTAGAGGACCAGCTT AGTGAAATAAAAACAAAGGAAGAAGAGCAACAACGCTTAATAAATGAGTTGTCAGCCCAGAAGGCACGTT TACACACAGAATCAGGTGAGTTTTCACGACAGCTAGATGAAAAAGATGCTATGGTTTCTCAGCTATCCCG AGGCAAACAAGCATTTACACAACAGATTGAAGAATTAAAGAGGCAGCTAGAAGAGGAGACTAAGGCCAAG AGCACTCTGGCCCATGCCCTGCAGTCAGCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGG AGCAGGAAGCCAAGGCTGAGCTGCAGAGGGGAATGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGAC CAAGTACGAGACGGACGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTAGCCCAGCGT CTGCAGGATGCAGAAGAACATGTAGAAGCTGTGAATTCCAAATGTGCTTCTCTTGAAAAGACAAAGCAGA GGCTACAGAATGAAGTAGAGGACCTCATGATTGATGTGGAACGATCTAATGCTGCCTGCATAGCTCTCGA TAAGAAGCAAAGAAACTTTGACAAGGTTCTGGCAGAATGGAAACAGAAGTATGAGGAAACTCAGGCTGAA CTTGAGGCCTCCCAGAAGGAGTCGCGTTCTCTCAGCACTGAGCTGTTCAAGGTGAAGAATGCCTACGAGG AATCCCTGGATCATCTTGAAACTCTAAAGCGAGAGAATAAGAACTTACAACAGGAGATTTCTGACCTGAC AGAGCAAATTGCAGAGGGTGGAAAGCATATCCATGAACTGGAGAAAGTAAAGAAACAACTTGATCATGAG AAGAGTGAACTACAGACTTCCCTAGAGGAAGCAGAGGCATCTCTTGAGCATGAAGAAGGCAAAATTCTTC GCATTCAACTTGAGCTAAATCAGGTGAAATCTGAGATTGACCGAAAAATTGCTGAAAAAGATGAAGAACT CGATCAGCTAAAGAGGAACCATCTCAGAGTTGTGGAGTCAATGCAGAGTACACTGGATGCTGAGATCAGG AGCAGAAATGATGCTCTGAGGATCAAGAAGAAGATGGAGGGAGATCTTAATGAAATGGAAATCCAGCTGA ACCATGCCAACCGCCAGGCTGCTGAGGCACTAAGGAATCTTAGAAACACACAAGGAATACTGAAGGACAC TCAGCTACATTTGGATGATGCCATCAGAGGCCAAGATGACCTTAAGGAACAACTGGCAATGGTTGAGCGC AGAGCTAACCTGATGCAGGCTGAAGTTGAAGAGCTCAGGGCATCCCTGGAACGGACTGAGAGAGGCAGGA AAATGGCAGAGCAAGAGCTTCTGGATGCCAGTGAACGTGTGCAACTTCTGCACACTCAGAACACCAGCCT GATCAACACCAAGAAGAAGCTGGAAACAGACATTTCCCAAATCCAGGGAGAGATGGAGGACATCGTCCAG GAAGCCCGCAATGCAGAGGAGAAGGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGA AGAAGGAACAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTGAAGGATCT GCAGCTCCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAG GCCAGGGTGAGAGAGCTTGAAAGTGAGGTGGAAAGTGAACAGAAGCACAATGTTGAGGCTGTCAAGGGTC TTCGCAAACATGAGAGAAGAGTGAAGGAACTCACTTACCAGACTGAGGAGGACCGCAAGAATATTCTCAG GCTGCAGGACTTGGTGGACAAATTGCAAACCAAAGTCAAAGCTTACAAGAGACAAGCTGAAGAGGCTGAG GAACAATCCAATGTCAACCTTGCCAAGTTCCGCAAGCTCCAGCACGAGCTGGAGGAGGCCAAGGAACGGG CTGACATTGCTGAGTCCCAAGTCAACAAGCTGAGAGTGAAGAGTCGGGAGGTTCACACAAAAGTCATAAG TGAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC220101 representing NM_017533 Red=Cloning site Green=Tags(s) MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKVTAKTEAGATV TVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVV TAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEP ASGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQL KAERSYHIFYQILSNKKPELIEMLLITTNPYDFAFVSQGEITVPSIDDQEELMATDSAVDILGFTADEKV AIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTV QQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL GKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLAFLFSGAQTA EAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCN GVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFK AGLLGTLEEMRDEKLAQLITRTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKI KPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADALADAEERCD QLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVK NLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLCM DLERAKRKLEGDLKLAQESTMDTENDKQQLNEKLKKKEFEMSNLQGKIEDEQALAIQLQKKIKELQARIE ELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEESTLQHE ATAAALRKKHADSVAELGEQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQL SEIKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEETKAK STLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQR LQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACIALDKKQRNFDKVLAEWKQKYEETQAE LEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHE KSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIR SRNDALRIKKKMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQDDLKEQLAMVER RANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQ EARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLE ARVRELESEVESEQKHNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQTKVKAYKRQAEEAE EQSNVNLAKFRKLQHELEEAKERADIAESQVNKLRVKSREVHTKVISEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017533
ORF Size	5817 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017533.2, NP_060003.2
RefSeq Size	6016 bp
RefSeq ORF	5820 bp
Locus ID	4622
UniProt ID	Q9Y623
Protein Pathways	Tight junction, Viral myocarditis
MW	222.9 kDa
Gene Summary	Muscle contraction.[UniProtKB/Swiss-Prot Function]

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