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RAD54 (RAD54L) (NM_003579) Human Tagged ORF Clone
CAT＃: RC220046
RAD54L (Myc-DDK-tagged)-Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1
ORF Plasmid: tGFP
"NM_003579" in other vectors (6)
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CNY 5,472.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hHR54; HR54; hRAD54; RAD54A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220046 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGGAGCTTGGCTCCCAGCCAGCTGGCCAAGAGAAAACCTGAAGGCAGGTCCTGTGATGATGAAG ACTGGCAACCTGGCCTAGTGACTCCTAGGAAACGGAAATCCAGCAGTGAGACCCAGATCCAGGAGTGTTT CCTGTCTCCTTTTCGGAAACCTTTGAGTCAGCTAACCAATCAACCACCTTGTCTGGACAGCAGTCAGCAT GAAGCATTTATTCGAAGCATTTTGTCAAAGCCTTTCAAAGTCCCCATTCCAAATTATCAAGGTCCTCTGG GCTCTCGAGCATTGGGCCTGAAAAGGGCTGGGGTCCGCCGGGCCCTCCATGACCCCCTGGAAAAAGATGC CTTGGTTCTGTATGAGCCTCCCCCGCTGAGCGCTCATGACCAGCTGAAGCTTGACAAGGAGAAACTCCCT GTCCATGTGGTTGTTGACCCTATTCTCAGTAAGGTTTTGCGGCCTCATCAGAGAGAGGGAGTGAAATTCC TGTGGGAGTGTGTCACCAGTCGGCGCATCCCTGGCAGCCATGGCTGCATCATGGCTGATGAGATGGGCCT AGGAAAGACGCTGCAGTGCATCACATTGATGTGGACACTTTTACGCCAGAGTCCAGAGTGCAAGCCAGAA ATTGACAAGGCAGTGGTGGTGTCGCCTTCCAGCCTGGTGAAGAACTGGTACAATGAGGTTGGGAAATGGC TCGGAGGGAGGATCCAACCTCTGGCCATCGATGGAGGATCTAAGGATGAAATAGACCAAAAGCTGGAAGG ATTCATGAACCAGCGTGGAGCCAGGGTGTCTTCTCCCATCCTCATCATTTCCTATGAGACCTTCCGCCTT CATGTTGGAGTCCTCCAGAAAGGAAGTGTTGGTCTGGTCATATGTGACGAGGGACACAGGCTCAAGAACT CTGAGAATCAGACTTACCAAGCCCTGGACAGCTTGAACACCAGCCGGCGGGTGCTCATCTCCGGAACTCC CATCCAGAATGATCTGCTTGAGTATTTCAGCTTGGTACATTTTGTTAATTCCGGCATCCTAGGGACTGCC CATGAATTCAAGAAGCATTTTGAATTGCCAATTTTGAAGGGTCGAGACGCTGCTGCTAGTGAGGCAGACA GGCAGCTAGGAGAGGAGCGGCTGCGGGAGCTCACCAGCATTGTGAATAGATGCCTGATACGGAGGACTTC TGATATCCTTTCTAAATATCTGCCTGTGAAGATTGAGCAGGTCGTTTGTTGTAGGCTGACACCCCTTCAG ACTGAGTTATACAAGAGGTTTCTGAGACAAGCCAAACCGGCAGAAGAATTGCTTGAGGGCAAGATGAGTG TGTCTTCCCTTTCTTCCATCACCTCGCTAAAGAAGCTTTGTAATCATCCAGCTCTAATCTATGATAAGTG TGTGGAAGAGGAGGATGGCTTTGTGGGTGCCTTGGACCTCTTCCCTCCTGGTTACAGCTCTAAGGCCCTG GAGCCCCAGCTGTCAGGTAAGATGCTGGTCCTGGATTATATTCTGGCGGTGACCCGAAGCCGTAGCAGTG ACAAAGTAGTGCTGGTGTCGAATTACACCCAGACTTTGGATCTCTTTGAGAAGCTGTGCCGTGCCCGAAG GTACTTATACGTCCGCCTGGATGGCACGATGTCCATTAAGAAGCGAGCCAAGGTTGTAGAACGCTTCAAT AGTCCATCGAGCCCTGACTTTGTCTTCATGCTGAGCAGCAAAGCTGGGGGCTGTGGCCTCAATCTCATTG GGGCTAACCGGCTGGTCATGTTTGACCCTGACTGGAACCCAGCCAATGATGAACAAGCCATGGCCCGGGT CTGGCGAGATGGTCAAAAGAAGACTTGCTATATCTACCGCCTGCTGTCTGCAGGGACCATTGAGGAGAAG ATCTTCCAGCGTCAGAGCCACAAGAAGGCACTGAGCAGCTGTGTGGTGGATGAGGAGCAGGATGTAGAGC GCCACTTCTCTCTGGGCGAGTTGAAGGAGCTGTTTATCCTGGATGAAGCTAGCCTCAGTGACACACATGA CAGGTTGCACTGCCGACGTTGTGTCAACAGCCGTCAGATCCGGCCACCCCCTGATGGTTCTGACTGCACT TCAGACCTGGCAGGGTGGAACCACTGCACTGATAAGTGGGGGCTCCGGGATGAGGTACTCCAGGCTGCCT GGGATGCTGCCTCCACTGCTATCACCTTCGTCTTCCACCAGCGTTCTCATGAGGAGCAGCGGGGCCTCCG C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220046 protein sequence
Red=Cloning site Green=Tags(s) MRRSLAPSQLAKRKPEGRSCDDEDWQPGLVTPRKRKSSSETQIQECFLSPFRKPLSQLTNQPPCLDSSQH EAFIRSILSKPFKVPIPNYQGPLGSRALGLKRAGVRRALHDPLEKDALVLYEPPPLSAHDQLKLDKEKLP VHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPE IDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGARVSSPILIISYETFRL HVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTA HEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQ TELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKAL EPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFN SPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEK IFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCT SDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003579 |
| ORF Size | 2241 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003579.4 |
| RefSeq Size | 3164 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 8438 |
| UniProt ID | Q92698 |
| Domains | SNF2_N, DEAD, helicase_C |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Homologous recombination |
| MW | 84.4 kDa |
| Gene Summary | The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220046L1 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC220046L2 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC220046L3 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC220046L4 | Lenti ORF clone of Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG220046 | RAD54L (tGFP-tagged) - Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1 |
CNY 6,370.00 |
|
| SC117863 | RAD54L (untagged)-Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 1 |
CNY 5,480.00 |
