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Kv4.3 (KCND3) (NM_172198) Human Tagged ORF Clone
CAT＃: RC219447
- TrueORF®
KCND3 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2
ORF Plasmid: tGFP
"NM_172198" in other vectors (4)
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CNY 4,560.00
| Cited in 1 publication. |
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CNY 300.00
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| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BRGDA9; KCND3L; KCND3S; KSHIVB; KV4.3; SCA19; SCA22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219447 representing NM_172198.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCCGGAGTTGCGGCCTGGCTGCCTTTTGCCCGGGCTGCGGCCATCGGGTGGATGCCGGTGGCC AACTGCCCCATGCCCCTGGCCCCGGCCGACAAGAACAAGCGGCAGGATGAGCTGATTGTCCTCAACGTG AGTGGGCGGAGGTTCCAGACCTGGAGGACCACGCTGGAGCGCTACCCGGACACCCTGCTGGGCAGCACG GAGAAGGAGTTCTTCTTCAACGAGGACACCAAGGAGTACTTCTTCGACCGGGACCCCGAGGTGTTCCGC TGCGTGCTCAACTTCTACCGCACGGGGAAGCTGCACTACCCGCGCTACGAGTGCATCTCTGCCTACGAC GACGAGCTGGCCTTCTACGGCATCCTCCCGGAGATCATCGGGGACTGCTGCTACGAGGAGTACAAGGAC CGCAAGAGGGAGAACGCCGAGCGGCTCATGGACGACAACGACTCGGAGAACAACCAGGAGTCCATGCCC TCGCTCAGCTTCCGCCAGACCATGTGGCGGGCCTTCGAGAACCCCCACACCAGCACGCTGGCCCTGGTC TTCTACTACGTGACTGGCTTCTTCATCGCTGTCTCGGTCATCACCAACGTGGTGGAGACGGTGCCGTGC GGCACGGTCCCGGGCAGCAAGGAGCTGCCGTGCGGGGAGCGCTACTCGGTGGCCTTCTTCTGCCTGGAC ACGGCGTGCGTCATGATCTTCACCGTGGAGTACCTCCTGCGGCTCTTCGCGGCTCCCAGCCGCTACCGC TTCATCCGCAGCGTCATGAGCATCATCGACGTGGTGGCCATCATGCCCTACTACATCGGTCTGGTCATG ACCAACAACGAGGACGTGTCCGGCGCCTTCGTCACGCTCCGGGTCTTCCGCGTCTTCAGGATCTTCAAG TTTTCCCGCCACTCCCAGGGCCTGCGGATCCTGGGCTACACACTGAAGAGCTGTGCCTCCGAACTGGGC TTTCTTCTCTTCTCCCTCACCATGGCCATCATCATCTTTGCCACTGTGATGTTTTATGCCGAGAAGGGC TCCTCGGCCAGCAAGTTCACAAGCATCCCTGCCTCGTTTTGGTACACCATTGTCACCATGACCACACTG GGATACGGAGACATGGTGCCTAAGACGATTGCAGGGAAGATCTTCGGCTCCATCTGCTCCTTGAGTGGC GTCCTGGTCATTGCCCTGCCAGTCCCTGTGATTGTTTCCAACTTTAGCCGGATTTACCACCAGAATCAG AGAGCTGATAAACGCAGGGCACAAAAGAAGGCCCGCCTTGCCAGGATCCGTGTGGCCAAAACAGGCAGT TCGAATGCATACCTGCACAGCAAGCGCAACGGGCTCCTCAACGAGGCGCTGGAGCTGACGGGCACCCCA GAAGAGGAGCACATGGGCAAGACCACCTCACTCATCGAGAGCCAGCATCATCACCTGCTGCACTGCCTG GAAAAAACCACTAACCACGAGTTTATTGATGAGCAGATGTTTGAGCAGAACTGCATGGAGAGTTCAATG CAGAACTACCCATCCACAAGAAGTCCCTCACTGTCCAGCCACCCAGGCCTCACTACCACCTGCTGCTCC CGTCGTAGTAAGAAGACCACACACCTGCCCAATTCTAACCTGCCAGCTACTCGCCTGCGCAGCATGCAA GAGCTCAGCACGATCCACATCCAGGGCAGTGAGCAGCCCTCCCTCACAACCAGTCGCTCCAGCCTTAAT TTGAAAGCAGACGACGGACTGAGACCAAACTGCAAAACATCCCAGATCACCACAGCCATCATCAGCATC CCCACTCCCCCAGCGCTAACCCCAGAGGGGGAAAGTCGGCCACCCCCTGCCAGCCCAGGCCCCAACACG AACATTCCTTCCATAGCCAGCAATGTTGTCAAGGTCTCCGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC219447
Blue=ORF Red=Cloning site Green=Tag(s) MAAGVAAWLPFARAAAIGWMPVANCPMPLAPADKNKRQDELIVLNVSGRRFQTWRTTLERYPDTLLGST EKEFFFNEDTKEYFFDRDPEVFRCVLNFYRTGKLHYPRYECISAYDDELAFYGILPEIIGDCCYEEYKD RKRENAERLMDDNDSENNQESMPSLSFRQTMWRAFENPHTSTLALVFYYVTGFFIAVSVITNVVETVPC GTVPGSKELPCGERYSVAFFCLDTACVMIFTVEYLLRLFAAPSRYRFIRSVMSIIDVVAIMPYYIGLVM TNNEDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKG SSASKFTSIPASFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQ RADKRRAQKKARLARIRVAKTGSSNAYLHSKRNGLLNEALELTGTPEEEHMGKTTSLIESQHHHLLHCL EKTTNHEFIDEQMFEQNCMESSMQNYPSTRSPSLSSHPGLTTTCCSRRSKKTTHLPNSNLPATRLRSMQ ELSTIHIQGSEQPSLTTSRSSLNLKADDGLRPNCKTSQITTAIISIPTPPALTPEGESRPPPASPGPNT NIPSIASNVVKVSAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172198 |
| ORF Size | 1908 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172198.3 |
| RefSeq Size | 2636 bp |
| RefSeq ORF | 1911 bp |
| Locus ID | 3752 |
| UniProt ID | Q9UK17 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 71.4 kDa |
| Gene Summary | Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member includes two isoforms with different sizes, which are encoded by alternatively spliced transcript variants of this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A polybasic motif in alternatively spliced KChIP2 isoforms prevents Ca2+ regulation of Kv4 channels
,Murphy, JG;Hoffman, DA;,
J. Biol. Chem.
,PubMed ID 30622142
[Kv4.3]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219447L3 | Lenti ORF clone of Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC219447L4 | Lenti ORF clone of Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG219447 | KCND3 (tGFP-tagged) - Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2 |
CNY 5,040.00 |
|
| SC306736 | KCND3 (untagged)-Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2 |
CNY 7,220.00 |
