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ME2 (NM_002396) Human Tagged ORF Clone
CAT＃: RC219389
ME2 (Myc-DDK-tagged)-Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid: tGFP
"NM_002396" in other vectors (6)
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CNY 4,352.00
CNY 5,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ODS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219389 representing NM_002396
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTCCCGGTTAAGAGTAGTTTCCACCACTTGTACTTTGGCATGTCGACATTTGCACATAAAAGAAA AAGGCAAGCCACTTATGCTGAACCCAAGAACAAACAAGGGAATGGCATTTACTTTACAAGAACGACAAAT GCTTGGTCTTCAAGGACTTCTACCTCCCAAAATAGAGACACAAGATATTCAAGCCTTACGATTTCATAGA AACTTGAAGAAAATGACTAGCCCTTTGGAAAAATATATCTACATAATGGGAATACAAGAAAGAAATGAGA AATTGTTTTATAGAATACTGCAAGATGACATTGAGAGTTTAATGCCAATTGTATATACACCGACGGTTGG TCTTGCCTGCTCCCAGTATGGACACATCTTTAGAAGACCTAAGGGATTATTTATTTCGATCTCAGACAGA GGTCATGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTAGTGACTGATGGAG AGAGAATTCTGGGTCTTGGAGATCTGGGTGTCTATGGAATGGGAATTCCAGTAGGAAAACTTTGTTTGTA TACAGCTTGTGCAGGAATACGGCCTGATAGATGCCTGCCAGTGTGTATTGATGTGGGAACTGATAATATC GCACTCTTAAAAGACCCATTTTACATGGGCTTGTACCAGAAACGAGATCGCACACAACAGTATGATGACC TGATTGATGAGTTTATGAAAGCTATTACTGACAGATATGGCCGGAACACACTCATTCAGTTCGAAGACTT TGGAAATCATAATGCATTCAGGTTCTTGAGAAAGTACCGAGAAAAATATTGTACTTTCAATGATGATATT CAAGGGACAGCTGCAGTAGCTCTAGCAGGTCTTCTTGCAGCACAAAAAGTTATTAGTAAACCAATCTCCG AACACAAAATCTTATTCCTTGGAGCAGGAGAGGCTGCTCTTGGAATTGCAAATCTTATAGTTATGTCTAT GGTAGAAAATGGCCTGTCAGAACAAGAGGCACAAAAGAAAATCTGGATGTTTGACAAGTATGGTTTATTA GTTAAGGGACGGAAAGCAAAAATAGATAGTTATCAGGAACCATTTACTCACTCAGCCCCAGAGAGCATAC CTGATACTTTTGAAGATGCAGTGAATATACTGAAGCCTTCAACTATAATTGGAGTTGCAGGTGCTGGCCG TCTTTTCACTCCTGATGTAATCAGAGCCATGGCCTCTATCAATGAAAGGCCTGTAATATTTGCATTAAGT AATCCTACAGCACAGGCAGAGTGCACGGCTGAAGAAGCATATACACTTACAGAGGGCAGGTGTTTGTTTG CCAGTGGCAGTCCATTTGGGCCAGTGAAACTTACAGATGGGCGAGTCTTTACACCAGGTCAAGGAAACAA TGTTTATATTTTTCCAGGTGTGGCTTTAGCTGTTATTCTCTGTAACACCCGGCATATTAGTGACAGTGTT TTCCTAGAAGCTGCAAAGGCCCTGACAAGCCAATTGACAGATGAAGAGCTAGCCCAAGGGAGACTTTACC CACCGCTTGCTAATATTCAGGAAGTTTCTATTAACATTGCTATTAAAGTTACAGAATACCTATATGCTAA TAAAATGGCTTTCCGATACCCAGAACCTGAAGACAAGGCCAAATATGTTAAAGAAAGAACATGGCGGAGT GAATATGATTCCCTGCTGCCAGATGTGTATGAATGGCCAGAATCTGCATCAAGCCCTCCTGTGATAACAG AA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219389 representing NM_002396
Red=Cloning site Green=Tags(s) MLSRLRVVSTTCTLACRHLHIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHR NLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDR GHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNI ALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDI QGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLL VKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALS NPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSV FLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRS EYDSLLPDVYEWPESASSPPVITE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002396 |
| ORF Size | 1752 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002396.5 |
| RefSeq Size | 2730 bp |
| RefSeq ORF | 1755 bp |
| Locus ID | 4200 |
| UniProt ID | P23368 |
| Domains | malic |
| Protein Pathways | Pyruvate metabolism |
| MW | 65.44 kDa |
| Gene Summary | This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219389L1 | Lenti ORF clone of Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 6,752.00 |
|
| RC219389L2 | Lenti ORF clone of Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 7,030.00 |
|
| RC219389L3 | Lenti ORF clone of Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 6,752.00 |
|
| RC219389L4 | Lenti ORF clone of Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 7,030.00 |
|
| RG219389 | ME2 (tGFP-tagged) - Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,610.00 |
|
| SC118666 | ME2 (untagged)-Human malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,360.00 |
