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ABCF1 (NM_001025091) Human Tagged ORF Clone
CAT＃: RC219085
ABCF1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family F (GCN20), member 1 (ABCF1), transcript variant 1
ORF Plasmid: tGFP
"NM_001025091" in other vectors (6)
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CNY 9,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ABC27; ABC50 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219085 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAGGCGCCCAAGCAGCAGCCGCCGGAGCCCGAGTGGATCGGGGACGGAGAGAGCACGAGCCCAT CAGACAAAGTGGTGAAGAAAGGGAAGAAGGACAAGAAGATCAAAAAAACGTTCTTTGAAGAGCTGGCAGT AGAAGATAAACAGGCTGGGGAAGAAGAGAAAGTGCTCAAGGAGAAGGAGCAGCAGCAGCAGCAACAGCAA CAGCAGCAAAAAAAAAAGCGAGATACCCGAAAAGGCAGGCGGAAGAAGGATGTGGATGATGATGGAGAAG AGAAAGAGCTCATGGAGCGTCTTAAGAAGCTCTCAGTGCCAACCAGTGATGAGGAGGATGAAGTACCCGC CCCAAAACCCCGCGGAGGGAAGAAAACCAAGGGTGGTAATGTTTTTGCAGCCCTGATTCAGGATCAGAGT GAGGAAGAGGAGGAGGAAGAAAAACATCCTCCTAAGCCTGCCAAGCCGGAGAAGAATCGGATCAATAAGG CCGTATCTGAGGAACAGCAGCCTGCACTCAAGGGCAAAAAGGGAAAGGAAGAGAAGTCAAAAGGGAAGGC TAAGCCTCAAAATAAATTCGCTGCTCTGGACAATGAAGAGGAGGATAAAGAAGAAGAAATTATAAAGGAA AAGGAGCCTCCCAAACAAGGGAAGGAGAAGGCCAAGAAGGCAGAGCAGGGTTCAGAGGAAGAAGGAGAAG GGGAAGAAGAGGAGGAGGAAGGAGGAGAGTCTAAGGCAGATGATCCCTATGCTCATCTTAGCAAAAAGGA GAAGAAAAAGCTGAAAAAACAGATGGAGTATGAGCGCCAAGTGGCTTCATTAAAAGCAGCCAATGCAGCT GAAAATGACTTCTCCGTGTCCCAGGCGGAGATGTCCTCCCGCCAAGCCATGTTAGAAAATGCATCTGACA TCAAGCTGGAGAAGTTCAGCATCTCCGCTCATGGCAAGGAGCTGTTCGTCAATGCAGACCTGTACATTGT AGCCGGCCGCCGCTACGGGCTGGTAGGACCCAATGGCAAGGGCAAGACCACACTCCTCAAGCACATTGCC AACCGAGCCCTGAGCATCCCTCCCAACATTGATGTGTTGCTGTGTGAGCAGGAGGTGGTAGCAGATGAGA CACCAGCAGTCCAGGCTGTTCTTCGAGCTGACACCAAGCGATTGAAGCTGCTGGAAGAGGAGCGGCGGCT TCAGGGACAGCTGGAACAAGGGGATGACACAGCTGCTGAGAGGCTAGAGAAGGTGTATGAGGAATTGCGG GCCACTGGGGCGGCAGCTGCAGAGGCCAAAGCACGGCGGATCCTGGCTGGCCTGGGCTTTGACCCTGAAA TGCAGAATCGACCCACACAGAAGTTCTCAGGGGGCTGGCGCATGCGTGTCTCCCTGGCCAGGGCACTGTT CATGGAGCCCACACTGCTGATGCTGGATGAGCCCACCAACCACCTGGACCTCAACGCTGTCATCTGGCTT AATAACTACCTCCAGGGCTGGCGGAAGACCTTGCTGATCGTCTCCCATGACCAGGGCTTCTTGGATGATG TCTGCACTGATATCATCCACCTCGATGCCCAGCGGCTCCACTACTATAGGGGCAATTACATGACCTTCAA AAAGATGTACCAGCAGAAGCAGAAAGAACTGCTGAAACAGTATGAGAAGCAAGAGAAAAAGCTGAAGGAG CTGAAGGCAGGCGGGAAGTCCACCAAGCAGGCGGAAAAACAAACGAAGGAAGCCCTGACTCGGAAGCAGC AGAAATGCCGACGGAAAAACCAAGATGAGGAATCCCAGGAGGCCCCTGAGCTCCTGAAGCGCCCTAAGGA GTACACTGTGCGCTTCACTTTTCCAGACCCCCCACCACTCAGCCCTCCAGTGCTGGGTCTGCATGGTGTG ACATTCGGCTACCAGGGACAGAAACCACTCTTTAAGAACTTGGATTTTGGCATCGACATGGATTCAAGGA TTTGCATTGTGGGCCCTAATGGTGTGGGGAAGAGTACGCTACTCCTGCTGCTGACTGGCAAGCTGACACC GACCCATGGGGAAATGAGAAAGAACCACCGGCTGAAAATTGGCTTCTTCAACCAGCAGTATGCAGAGCAG CTGCGCATGGAGGAGACGCCCACTGAGTACCTGCAGCGGGGCTTCAACCTGCCCTACCAGGATGCCCGCA AGTGCCTGGGCCGCTTCGGCCTGGAGAGTCACGCCCACACCATCCAGATCTGCAAACTCTCTGGTGGTCA GAAGGCGCGAGTTGTGTTTGCTGAGCTGGCCTGTCGGGAACCTGATGTCCTCATCTTGGACGAGCCAACC AATAACCTGGACATAGAGTCTATTGATGCTCTAGGGGAGGCCATCAATGAATACAAGGGTGCTGTGATCG TTGTCAGCCATGATGCCCGACTCATCACAGAAACCAATTGCCAGCTGTGGGTGGTGGAGGAGCAGAGTGT TAGCCAAATCGATGGTGACTTTGAAGACTACAAGCGGGAGGTGTTGGAGGCCCTGGGTGAAGTCATGGTC AGCCGGCCCCGAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219085 protein sequence
Red=Cloning site Green=Tags(s) MPKAPKQQPPEPEWIGDGESTSPSDKVVKKGKKDKKIKKTFFEELAVEDKQAGEEEKVLKEKEQQQQQQQ QQQKKKRDTRKGRRKKDVDDDGEEKELMERLKKLSVPTSDEEDEVPAPKPRGGKKTKGGNVFAALIQDQS EEEEEEEKHPPKPAKPEKNRINKAVSEEQQPALKGKKGKEEKSKGKAKPQNKFAALDNEEEDKEEEIIKE KEPPKQGKEKAKKAEQGSEEEGEGEEEEEEGGESKADDPYAHLSKKEKKKLKKQMEYERQVASLKAANAA ENDFSVSQAEMSSRQAMLENASDIKLEKFSISAHGKELFVNADLYIVAGRRYGLVGPNGKGKTTLLKHIA NRALSIPPNIDVLLCEQEVVADETPAVQAVLRADTKRLKLLEEERRLQGQLEQGDDTAAERLEKVYEELR ATGAAAAEAKARRILAGLGFDPEMQNRPTQKFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWL NNYLQGWRKTLLIVSHDQGFLDDVCTDIIHLDAQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKE LKAGGKSTKQAEKQTKEALTRKQQKCRRKNQDEESQEAPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGV TFGYQGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTHGEMRKNHRLKIGFFNQQYAEQ LRMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPT NNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFEDYKREVLEALGEVMV SRPRE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025091 |
| ORF Size | 2535 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025091.2 |
| RefSeq Size | 3474 bp |
| RefSeq ORF | 2538 bp |
| Locus ID | 23 |
| UniProt ID | Q8NE71 |
| Protein Families | Druggable Genome |
| MW | 95.9 kDa |
| Gene Summary | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219085L1 | Lenti ORF clone of Human ATP-binding cassette, sub-family F (GCN20), member 1 (ABCF1), transcript variant 1, Myc-DDK-tagged |
CNY 11,680.00 |
|
| RC219085L2 | Lenti ORF clone of Human ATP-binding cassette, sub-family F (GCN20), member 1 (ABCF1), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RC219085L3 | Lenti ORF clone of Human ATP-binding cassette, sub-family F (GCN20), member 1 (ABCF1), transcript variant 1, Myc-DDK-tagged |
CNY 11,680.00 |
|
| RC219085L4 | Lenti ORF clone of Human ATP-binding cassette, sub-family F (GCN20), member 1 (ABCF1), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RG219085 | ABCF1 (tGFP-tagged) - Human ATP-binding cassette, sub-family F (GCN20), member 1 (ABCF1), transcript variant 1 |
CNY 10,880.00 |
|
| SC302344 | ABCF1 (untagged)-Human ATP-binding cassette, sub-family F (GCN20), member 1 (ABCF1), transcript variant 1 |
CNY 7,220.00 |
