United States
Germany
Japan
United Kingdom
China
EYA1 (NM_172060) Human Tagged ORF Clone
CAT＃: RC218084
EYA1 (Myc-DDK-tagged)-Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 1
ORF Plasmid: tGFP
"NM_172060" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 6,712.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BOP; BOR; BOS1; OFC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218084 representing NM_172060
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCTGTTTCCTCAAGTTGCAGTTAAAACAGAGCCAATGAGCAGCAGTGAAACAGCTTCAACGACAG CCGACGGGTCTTTAAACAATTTCTCAGGTTCAGCAATTGGGAGCAGTAGTTTCAGCCCACGACCAACTCA CCAGTTCTCTCCACCACAGATTTACCCTTCCAACAGACCATACCCACATATTCTCCCTACCCCTTCCTCA CAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGGAATGCAACAAGCTACAGCCTATGCCACGT ACCCACAGCCAGGACAGCCGTACGGCATTTCCTCATATGGCATCAAGACTGAAGGTGGATTGTCACAGTC TCAGTCACCTGGACAGACAGGATTTCTCAGCTATGGCACAAGCTTCAGTACCCCTCAACCTGGACAGGCA CCATACAGCTACCAGATGCAAGGTAGCAGTTTTACAACATCATCAGGAATATATACAGGAAATAATTCAC TCACAAATTCCTCTGGATTTAATAGTTCACAGCAGGACTATCCGTCTTATCCCAGTTTTGGCCAGGGTCA GTACGCACAGTATTATAACAGCTCACCGTATCCAGCACATTATATGACCAGCAGCAACACCAGCCCAACG ACACCATCCACCAATGCCACTTACCAGCTTCAAGAACCGCCATCTGGCATCACCAGCCAAGCAGTTACAG ATCCCACAGCAGAGTACAGCACAATCCACAGCCCATCAACACCCATTAAAGATTCAGATTCTGATCGATT GCGTCGAGGTTCAGATGGGAAATCACGTGGACGGGGCCGAAGAAACAATAATCCTTCACCTCCCCCAGAT TCTGATCTTGAGAGAGTGTTCATCTGGGACTTGGATGAGACAATCATTGTTTTCCACTCCTTGCTTACTG GGTCCTACGCCAACAGATATGGGAGGGATCCACCCACTTCAGTTTCCCTTGGACTGCGAATGGAAGAAAT GATTTTCAACTTGGCAGACACACATTTATTTTTTAATGACTTAGAAGAATGTGACCAAGTCCATATAGAT GATGTTTCTTCAGATGATAACGGACAGGACCTAAGCACATATAACTTTGGAACAGATGGCTTTCCTGCTG CAGCAACCAGTGCTAACTTATGTTTGGCAACTGGTGTACGGGGTGGTGTGGACTGGATGAGAAAGTTGGC CTTCCGCTACAGACGGGTAAAAGAGATCTACAACACCTACAAAAATAATGTTGGAGGTCTGCTTGGTCCA GCTAAGAGGGAAGCCTGGCTGCAGTTGAGGGCCGAAATTGAAGCCCTGACCGACTCCTGGTTGACACTGG CCCTGAAAGCACTCTCGCTCATTCACTCCCGGACAAACTGTGTGAATATTTTAGTAACAACTACTCAGCT CATCCCAGCATTGGCGAAAGTCCTGCTGTATGGGTTAGGAATTGTATTTCCAATAGAAAATATTTACAGT GCAACTAAAATAGGAAAAGAAAGCTGTTTTGAGAGAATAATTCAAAGGTTTGGAAGAAAAGTGGTGTATG TTGTTATAGGAGATGGTGTAGAAGAAGAACAAGGAGCAAAAAAAGCACGCGATGCCCTTCTGGAGGATCT CCAGCCACTCGGACCTCATGGCCCTGCACCACGCCTTGGAACTGGAGTACCTGTAACAGCGCTCGGCACT TTGACAGCGCACAGCTGCTCTGTGACCAGGGACAGATCCAGCAGGCCCCAGTCTCGCATCAGCGCCGGCC TCCAGAACTTAGCAATTCGAAGGAACACATTAAACTGGTGGATGCAGCAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218084 representing NM_172060
Red=Cloning site Green=Tags(s) MLLFPQVAVKTEPMSSSETASTTADGSLNNFSGSAIGSSSFSPRPTHQFSPPQIYPSNRPYPHILPTPSS QTMAAYGQTQFTTGMQQATAYATYPQPGQPYGISSYGIKTEGGLSQSQSPGQTGFLSYGTSFSTPQPGQA PYSYQMQGSSFTTSSGIYTGNNSLTNSSGFNSSQQDYPSYPSFGQGQYAQYYNSSPYPAHYMTSSNTSPT TPSTNATYQLQEPPSGITSQAVTDPTAEYSTIHSPSTPIKDSDSDRLRRGSDGKSRGRGRRNNNPSPPPD SDLERVFIWDLDETIIVFHSLLTGSYANRYGRDPPTSVSLGLRMEEMIFNLADTHLFFNDLEECDQVHID DVSSDDNGQDLSTYNFGTDGFPAAATSANLCLATGVRGGVDWMRKLAFRYRRVKEIYNTYKNNVGGLLGP AKREAWLQLRAEIEALTDSWLTLALKALSLIHSRTNCVNILVTTTQLIPALAKVLLYGLGIVFPIENIYS ATKIGKESCFERIIQRFGRKVVYVVIGDGVEEEQGAKKARDALLEDLQPLGPHGPAPRLGTGVPVTALGT LTAHSCSVTRDRSSRPQSRISAGLQNLAIRRNTLNWWMQQM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172060 |
| ORF Size | 1803 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 3734 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 2138 |
| UniProt ID | Q99502 |
| Protein Families | Druggable Genome, Phosphatase, Transcription Factors |
| MW | 61 kDa |
| Gene Summary | This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Dec 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218084L1 | Lenti ORF clone of Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 1, Myc-DDK-tagged |
CNY 9,112.00 |
|
| RC218084L2 | Lenti ORF clone of Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC218084L3 | Lenti ORF clone of Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC218084L4 | Lenti ORF clone of Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG218084 | EYA1 (tGFP-tagged) - Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 1 |
CNY 8,312.00 |
|
| SC109203 | EYA1 (untagged)-Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 1 |
CNY 6,256.00 |
