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Glutamyl Prolyl tRNA synthetase (EPRS) (NM_004446) Human Tagged ORF Clone
CAT＃: RC217559
EPRS (Myc-DDK-tagged)-Human glutamyl-prolyl-tRNA synthetase (EPRS)
ORF Plasmid: tGFP
"NM_004446" in other vectors (6)
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CNY 15,520.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32; QARS; QPRS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217559 representing NM_004446
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGCTCTCTCTGACCGTGAATTCAGGAGACCCTCCGCTAGGAGCTTTGCTGGCAGTAGAACACG TGAAAGACGATGTCAGCATTTCCGTTGAAGAAGGGAAAGAGAATATTCTTCATGTTTCTGAAAATGTGAT ATTCACAGATGTGAATTCTATACTTCGCTACTTGGCTAGAGTTGCAACTACAGCTGGGTTATATGGCTCT AATCTGATGGAACATACTGAGATTGATCACTGGTTGGAGTTCAGTGCTACAAAATTATCTTCATGTGATT CCTTTACTTCTACAATTAATGAACTCAATCATTGCCTGTCTCTGAGAACATACTTAGTTGGAAACTCCTT GAGTTTAGCAGATTTATGTGTTTGGGCCACCCTAAAAGGAAATGCTGCCTGGCAAGAACAGTTGAAACAG AAGAAAGCTCCAGTTCATGTAAAACGTTGGTTTGGCTTTCTTGAAGCCCAGCAGGCCTTCCAGTCAGTAG GTACCAAGTGGGATGTTTCAACAACCAAAGCTCGAGTGGCACCTGAGAAAAAGCAAGATGTTGGGAAATT TGTTGAGCTTCCAGGTGCGGAGATGGGAAAGGTTACCGTCAGATTTCCTCCAGAGGCCAGTGGTTACTTA CACATTGGGCATGCAAAAGCTGCTCTTCTGAACCAGCACTACCAGGTTAACTTTAAAGGGAAACTGATCA TGAGATTTGATGACACAAATCCTGAAAAAGAAAAGGAAGATTTTGAGAAGGTTATCTTGGAAGATGTTGC AATGTTGCATATCAAACCAGATCAATTTACTTATACTTCGGATCATTTTGAAACTATAATGAAGTATGCA GAGAAGCTAATTCAAGAAGGGAAGGCTTATGTGGATGATACTCCTGCTGAACAGATGAAAGCAGAACGTG AGCAGAGGATAGACTCTAAACATAGAAAAAACCCTATTGAGAAGAATCTACAAATGTGGGAAGAAATGAA AAAAGGGAGCCAGTTTGGTCAGTCCTGTTGTTTGCGAGCAAAAATTGACATGAGTAGTAACAATGGATGC ATGAGAGATCCAACCCTTTATCGCTGCAAAATTCAACCACATCCAAGAACTGGAAATAAATACAATGTTT ATCCAACATATGATTTTGCCTGCCCCATAGTTGACAGCATCGAAGGTGTTACACATGCCCTGAGAACAAC AGAATACCATGACAGAGATGAGCAGTTTTACTGGATTATTGAAGCTTTAGGCATAAGAAAACCATATATT TGGGAATATAGTCGGCTAAATCTCAACAACACAGTGCTATCCAAAAGAAAACTCACATGGTTTGTCAATG AAGGACTAGTAGATGGATGGGATGACCCAAGATTTCCTACGGTTCGTGGTGTACTGAGAAGAGGGATGAC AGTTGAAGGACTGAAACAGTTTATTGCTGCTCAGGGCTCCTCACGTTCAGTCGTGAACATGGAGTGGGAC AAAATCTGGGCGTTTAACAAAAAGGTTATTGACCCAGTGGCTCCACGATATGTTGCATTACTGAAGAAAG AAGTGATCCCAGTGAATGTACCTGAAGCTCAGGAGGAGATGAAAGAAGTAGCCAAACACCCAAAGAATCC TGAGGTTGGCTTGAAGCCTGTGTGGTATAGTCCCAAAGTTTTCATTGAAGGTGCTGATGCAGAGACTTTT TCGGAGGGTGAGATGGTTACATTTATAAATTGGGGCAACCTCAACATTACAAAAATACACAAAAATGCAG ATGGAAAAATCATATCTCTTGATGCAAAGTTGAATTTGGAAAACAAAGACTACAAGAAAACCACTAAGGT CACTTGGCTTGCAGAGACTACACATGCTCTTCCTATTCCAGTAATCTGTGTCACTTATGAGCACTTGATC ACAAAGCCAGTGCTAGGAAAAGACGAGGACTTTAAGCAGTATGTCAACAAGAACAGTAAGCATGAAGAGC TAATGCTAGGGGATCCCTGCCTTAAGGATTTGAAAAAAGGAGATATTATACAACTCCAGAGAAGAGGATT CTTCATATGTGATCAACCTTATGAACCTGTTAGCCCATATAGTTGCAAGGAAGCCCCGTGTGTTTTGATA TACATTCCTGATGGGCACACAAAGGAAATGCCAACATCAGGGTCAAAGGAAAAGACCAAAGTAGAAGCCA CAAAAAATGAGACCTCTGCTCCTTTTAAGGAAAGACCAACACCTTCTCTGAATAATAATTGTACTACATC TGAGGATTCCTTGGTCCTTTACAATAGAGTGGCTGTTCAAGGAGATGTGGTTCGTGAATTAAAAGCCAAG AAAGCACCAAAGGAAGATGTAGATGCAGCTGTAAAACAGCTTTTGTCTTTGAAAGCTGAATATAAGGAGA AAACTGGCCAGGAATATAAACCTGGAAACCCTCCTGCTGAAATAGGACAGAATATTTCTTCTAATTCCTC AGCAAGTATTCTGGAAAGTAAATCTCTGTATGATGAAGTTGCTGCACAAGGGGAGGTGGTTCGTAAGCTA AAAGCTGAAAAATCCCCTAAGGCTAAAATAAATGAAGCTGTAGAATGCTTACTGTCCCTGAAGGCTCAGT ATAAAGAAAAAACTGGGAAGGAGTACATACCTGGTCAGCCCCCATTATCTCAAAGTTCGGATTCAAGCCC AACCAGAAATTCTGAACCTGCTGGTTTAGAAACACCAGAAGCGAAAGTACTTTTTGACAAAGTAGCTTCT CAAGGGGAAGTAGTTCGGAAACTTAAAACTGAAAAAGCCCCTAAGGATCAAGTAGATATAGCTGTTCAAG AACTCCTTCAGCTAAAGGCACAGTACAAGTCTTTGATAGGAGTAGAGTATAAGCCTGTGTCGGCCACTGG AGCTGAGGACAAAGATAAGAAGAAGAAAGAAAAAGAAAATAAATCTGAAAAGCAGAATAAGCCTCAGAAA CAAAATGATGGCCAAAGGAAAGACCCTTCTAAAAACCAAGGAGGTGGGCTCTCATCAAGTGGAGCAGGAG AAGGGCAGGGGCCTAAGAAACAGACCAGGTTGGGTCTTGAGGCAAAAAAAGAAGAAAATCTTGCTGATTG GTATTCTCAGGTCATCACAAAGTCAGAAATGATTGAATACCATGACATAAGTGGCTGTTATATTCTTCGT CCCTGGGCCTATGCCATTTGGGAAGCCATCAAGGACTTTTTTGATGCTGAGATCAAGAAACTTGGTGTTG AAAACTGCTACTTCCCCATGTTTGTGTCTCAAAGTGCATTAGAGAAAGAGAAGACTCATGTTGCTGACTT TGCCCCAGAGGTTGCTTGGGTTACAAGATCTGGCAAAACCGAGCTGGCAGAACCAATTGCCATTCGTCCT ACTAGTGAAACAGTAATGTATCCTGCATATGCAAAATGGGTACAGTCACACAGAGACCTGCCCATCAAGC TCAATCAGTGGTGCAATGTGGTGCGTTGGGAATTCAAGCATCCTCAGCCTTTCCTACGTACTCGTGAATT TCTTTGGCAGGAAGGGCACAGTGCTTTTGCTACCATGGAAGAGGCAGCGGAAGAGGTCTTGCAGATACTT GACTTATATGCTCAGGTATATGAAGAACTCCTGGCAATTCCTGTTGTTAAAGGAAGAAAGACGGAAAAGG AAAAATTTGCAGGAGGAGACTATACAACTACAATAGAAGCATTTATATCTGCTAGTGGAAGAGCTATCCA GGGAGGAACATCACATCATTTAGGGCAGAATTTTTCCAAAATGTTTGAAATCGTTTTTGAAGATCCAAAG ATACCAGGAGAGAAGCAATTTGCCTATCAAAACTCCTGGGGCCTGACAACTCGAACTATTGGTGTTATGA CCATGGTTCATGGGGACAACATGGGTTTAGTATTACCACCCCGTGTAGCATGTGTTCAGGTGGTGATTAT TCCTTGTGGCATTACCAATGCACTTTCTGAAGAAGACAAAGAAGCGCTGATTGCAAAATGCAATGATTAT CGAAGGCGATTACTCAGTGTTAACATCCGCGTTAGAGCTGATTTACGAGATAATTATTCTCCAGGTTGGA AATTCAATCACTGGGAGCTCAAGGGAGTTCCCATTAGACTTGAAGTTGGGCCACGTGATATGAAGAGCTG TCAGTTTGTAGCCGTCAGACGAGATACTGGAGAAAAGCTGACAGTTGCTGAAAATGAGGCAGAGACTAAA CTTCAAGCTATTTTGGAAGACATCCAGGTCACCCTTTTCACAAGGGCTTCTGAAGACCTTAAGACTCATA TGGTTGTGGCTAATACAATGGAAGACTTTCAGAAGATACTAGATTCTGGAAAGATTGTTCAGATTCCATT CTGTGGGGAAATTGACTGTGAGGACTGGATCAAAAAGACCACTGCCAGGGATCAAGATCTTGAACCTGGT GCTCCATCCATGGGAGCTAAAAGCCTTTGCATCCCCTTCAAACCACTCTGTGAACTGCAGCCTGGAGCCA AATGTGTCTGTGGCAAGAACCCTGCCAAGTACTACACCTTATTTGGTCGCAGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217559 representing NM_004446
Red=Cloning site Green=Tags(s) MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGS NLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQ KKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYL HIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYA EKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGC MRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYI WEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWD KIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETF SEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLI TKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLI YIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAK KAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKL KAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVAS QGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQK QNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILR PWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRP TSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQIL DLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPK IPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDY RRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETK LQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPG APSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004446 |
| ORF Size | 4536 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004446.3 |
| RefSeq Size | 5021 bp |
| RefSeq ORF | 4539 bp |
| Locus ID | 2058 |
| UniProt ID | P07814 |
| Domains | WHEP-TRS, tRNA-synt_1c, tRNA-synt_2b, HGTP_anticodon |
| Protein Families | Druggable Genome |
| Protein Pathways | Aminoacyl-tRNA biosynthesis, Metabolic pathways, Porphyrin and chlorophyll metabolism |
| MW | 170.4 kDa |
| Gene Summary | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
EPRS1 Controls the TGF- β Signaling Pathway via Interaction with TβRI in Hepatic Stellate Cell
,null,
Molecular and Cellular Biology
,PubMed ID 37154023
[Glutamyl Prolyl tRNA synthetase]
|
|
EPRS/GluRS promotes gastric cancer development via WNT/GSK-3β/β-catenin signaling pathway
,Liu, H;Fredimoses, M;Niu, P;Liu, T;Qiao, Y;Tian, X;Chen, X;Kim, DJ;Li, X;Liu, K;Dong, Z;,
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association
,PubMed ID 33740160
[Glutamyl Prolyl tRNA synthetase]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217559L1 | Lenti ORF clone of Human glutamyl-prolyl-tRNA synthetase (EPRS), Myc-DDK-tagged |
CNY 17,010.00 |
|
| RC217559L2 | Lenti ORF clone of Human glutamyl-prolyl-tRNA synthetase (EPRS), mGFP tagged |
CNY 17,010.00 |
|
| RC217559L3 | Lenti ORF clone of Human glutamyl-prolyl-tRNA synthetase (EPRS), Myc-DDK-tagged |
CNY 17,010.00 |
|
| RC217559L4 | Lenti ORF clone of Human glutamyl-prolyl-tRNA synthetase (EPRS), mGFP tagged |
CNY 17,010.00 |
|
| RG217559 | EPRS (tGFP-tagged) - Human glutamyl-prolyl-tRNA synthetase (EPRS) |
CNY 17,120.00 |
|
| SC117359 | EPRS (untagged)-Human glutamyl-prolyl-tRNA synthetase (EPRS) |
CNY 15,528.00 |
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