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UBE4A (NM_004788) Human Tagged ORF Clone
CAT＃: RC217407
UBE4A (Myc-DDK-tagged)-Human ubiquitination factor E4A (UBE4A), transcript variant 1
ORF Plasmid: tGFP
"NM_004788" in other vectors (6)
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CNY 7,472.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E4; UBOX2; UFD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217407 representing NM_004788
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCTG ATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGCCC AGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGCCGC TCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTCCTTA TTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTATTTGGA AGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTCTTCGCT CGCTTATTACTTCAAGATCCAGGCAACCACTTAATTAACATGACTTCTTCTACAACGCTAAATCTCTCTG CTGATCGAGATGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAGCCAAGGAAGA GATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTGCAGTGCAGAAACCTCACTGTGTCCAATACC CGAACAGTTCTTCTCACCCCAGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGT TAGAAGCCATCCAGGGAGCCCGTGAGTACATGAACAAGATCTATTTTGAAGATGTAACTGAGTTTCTGGA AGAGGTCATTGAAGCCTTGATATTGGATGAGGAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTT GATATTTTATTGGGCCGAATAAAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATATCTGGATATTC TTCTCTATTTCACTAGGCAAAAAGATATGGCAAAGGTTTTTGTAGAATACATTCAGCCCAAGGACCCTAC CAATGGGCAAATGTACCAGAAGACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTAAAGACTCCG GGTGTTGTAGAAAATCATGGCTACTTTTTGAATCCATCTCGTTCCAGCCCCCAGGAGATCAAAGTACAGG AGGCCAACATCCATCAGTTCATGGCTCAGTTCCACGAAAAGATCTACCAGATGCTGAAGAACTTACTCCA GCTCTCTCCAGAAACCAAACACTGTATCTTGTCCTGGCTTGGAAACTGTTTGCATGCAAATGCAGGCCGC ACCAAGATTTGGGCCAATCAGATGCCAGAAATCTTTTTCCAAATGTATGCCTCAGATGCTTTCTTTCTGA ATCTGGGTGCTGCTCTCCTGAAGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTT TAATCCCACATACTGTGCCCTCAAGGAGTTGAATGATGAAGAACGAAAAATTAAAAATGTACACATGAGA GGTTTGGACAAAGAAACCTGTTTGATCCCAGCTGTGCAGGAGCCGAAGTTTCCACAGAACTACAACCTTG TAACAGAGAACCTTGCTCTGACAGAGTACACCTTGTACTTGGGATTTCACAGGTTGCATGATCAGATGGT AAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGGCGGGATGCTCAGCAAAGTTCTAGCCCTGCT GCTGACAATCTTCGTGAGCAGTTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATGACAG AGCCACAAATGCTACAAAACTGCCTAAACTTGCAGGTGTCCATGGCTGTTCTACTGGTTCAACTGGCCAT AGGCAATGAGGGCTCACAGCCAATAGAGCTAACCTTTCCTTTGCCAGATGGCTACAGCTCTTTGGCTTAT GTGCCAGAATTTTTTGCAGATAACCTGGGTGATTTTCTCATTTTTCTCCGCCGCTTTGCCGATGACATTT TGGAGACATCAGCAGATTCCCTGGAGCATGTCCTTCACTTTATCACCATTTTCACTGGAAGCATAGAAAG AATGAAGAATCCCCACCTGAGGGCCAAACTAGCAGAGGTGTTGGAAGCAGTGATGCCCCACCTGGATCAG ACCCCAAATCCCTTGGTATCCAGTGTGTTCCACCGGAAACGTGTGTTCTGCAACTTTCAGTATGCACCCC AACTTGCAGAGGCTCTAATCAAGGTTTTTGTGGACATCGAATTTACAGGAGACCCCCATCAATTTGAACA GAAGTTTAATTACCGCCGTCCCATGTATCCTATCCTAAGATACATGTGGGGGACAGATACCTATCGGGAG AGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAGAAGCCATGAATCCCCCACTTTTCCTCCGCT TTCTTAACCTGCTAATGAATGATGCCATCTTCCTTTTGGATGAAGCCATACAGTATTTGAGCAAGATAAA GATTCAGCAAATTGAGAAGGATCGAGGTGAATGGGATAGTCTGACTCCAGAAGCCCGCCGAGAAAAGGAG GCTGGCCTACAGATGTTTGGACAGCTGGCACGTTTCCATAACATCATGTCCAATGAAACAATCGGTACCC TTGCCTTTCTCACATCAGAGATCAAGTCACTCTTTGTGCATCCCTTCCTGGCTGAGCGCATCATCTCCAT GTTGAACTACTTCCTGCAACACCTGGTTGGCCCCAAGATGGGTGCCTTAAAAGTCAAGGACTTCAGCGAA TTTGACTTCAAACCCCAGCAGCTTGTATCAGATATCTGCACTATCTACTTAAATCTTGGGGATGAGGAGA ATTTCTGTGCCACTGTGCCCAAGGATGGACGTTCCTATTCCCCAACTCTCTTTGCACAGACAGTTCGAGT CTTGAAGAAAATAAATAAGCCTGGGAATATGATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCT CTTGCAGACCTCCAACAACAGGAAGAGGAAACCTATGCAGATGCCTGTGATGAGTTCCTGGATCCCATTA TGAGCACACTGATGTGTGACCCTGTGGTGCTGCCATCTTCCAGAGTCACTGTGGATAGATCCACCATTGC AAGACATTTGCTCAGTGACCAAACAGATCCCTTTAACCGTAGTCCCCTCACCATGGACCAGATCCGGCCA AACACAGAACTAAAAGAAAAAATCCAACGGTGGCTTGCAGAGAGGAAACAACAAAAGGAGCAACTTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217407 representing NM_004788
Red=Cloning site Green=Tags(s) MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISR SFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFA RLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNT RTVLLTPEIYVDQNIHEQLVDLMLEAIQGAREYMNKIYFEDVTEFLEEVIEALILDEEVRTFPEVMIPVF DILLGRIKDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTP GVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGR TKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMR GLDKETCLIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPA ADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAY VPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQ TPNPLVSSVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRE SIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKE AGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSE FDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIKS LADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRP NTELKEKIQRWLAERKQQKEQLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004788 |
| ORF Size | 3219 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004788.4 |
| RefSeq Size | 6138 bp |
| RefSeq ORF | 3222 bp |
| Locus ID | 9354 |
| UniProt ID | Q14139 |
| Domains | U-box |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 123.3 kDa |
| Gene Summary | This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217407L1 | Lenti ORF clone of Human ubiquitination factor E4A (UBE4A), transcript variant 1, Myc-DDK-tagged |
CNY 9,872.00 |
|
| RC217407L2 | Lenti ORF clone of Human ubiquitination factor E4A (UBE4A), transcript variant 1, mGFP tagged |
CNY 12,640.00 |
|
| RC217407L3 | Lenti ORF clone of Human ubiquitination factor E4A (UBE4A), transcript variant 1, Myc-DDK-tagged |
CNY 12,640.00 |
|
| RC217407L4 | Lenti ORF clone of Human ubiquitination factor E4A (UBE4A), transcript variant 1, mGFP tagged |
CNY 12,640.00 |
|
| RG217407 | UBE4A (tGFP-tagged) - Human ubiquitination factor E4A (UBE4A), transcript variant 1 |
CNY 11,780.00 |
|
| SC315144 | UBE4A (untagged)-Human ubiquitination factor E4A (UBE4A), transcript variant 1 |
CNY 7,480.00 |
