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FARP2 (NM_014808) Human Tagged ORF Clone
CAT＃: RC216784
- TrueORF®
FARP2 (Myc-DDK-tagged)-Human FERM, RhoGEF and pleckstrin domain protein 2 (FARP2)
ORF Plasmid: tGFP
"NM_014808" in other vectors (6)
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CNY 7,336.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FIR; FRG; PLEKHC3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216784 representing NM_014808
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAGATAGAAGGAACATACAGAGTCCTGCAGACTGCAGGGATGCGCTTGGGTGCCCAGACCCCTG TGGGAGTTAGCACCCTTGAGCCTGGGCAGACTCTCTTGCCCAGAATGCAAGAGAAGCACCTGCACCTCAG AGTAAAGCTGCTGGACAACACCATGGAAATATTTGACATTGAGCCTAAATGCGATGGCCAGGTATTACTG ACACAAGTGTGGAAGCGTTTAAACCTGGTAGAATGTGACTACTTCGGGATGGAGTTTCAAAATACTCAGT CCTACTGGATTTGGCTTGAACCTATGAAACCCATCATTAGGCAAATACGAAGGCCAAAGAATGTGGTGCT TCGCCTAGCTGTAAAATTTTTTCCACCTGATCCTGGTCAGCTACAAGAAGAATATACAAGATACTTGTTT GCCTTGCAACTTAAGAGAGACCTGCTGGAAGAGCGTTTGACCTGTGCTGACACCACAGCGGCCCTTCTCA CGTCCCATCTCCTGCAGTCGGAAATAGGAGATTACGATGAAACGCTGGACCGAGAGCACCTCAAAGTGAA CGAGTATTTGCCTGGCCAGCAGCACTGCCTTGAGAAGATACTAGAATTCCATCAGAAGCACGTGGGCCAG ACACCTGCTGAGTCGGATTTCCAGGTGCTCGAAATTGCTCGAAAGTTGGAAATGTACGGCATCAGATTTC ACATGGCTTCTGACAGGGAAGGAACCAAGATTCAACTGGCAGTTTCCCACATGGGTGTACTCGTGTTCCA GGGCACCACCAAAATCAACACTTTCAACTGGTCCAAGGTCCGTAAACTAAGCTTCAAGAGGAAAAGATTT CTTATCAAACTTCATCCAGAGGTTCATGGACCTTACCAGGACACATTAGAATTTTTGTTGGGTAGTAGAG ATGAATGTAAGAACTTCTGGAAGATTTGTGTGGAGTATCACACCTTTTTTAGACTTTTGGACCAACCTAA GCCAAAAGCAAAAGCCGTCTTCTTCAGCCGGGGCTCCTCCTTCAGATACAGTGGAAGAACTCAGAAACAA CTAGTAGATTATTTCAAAGACAGTGGAATGAAGAGAATTCCATATGAAAGAAGGCACAGCAAGACCCACA CGTCCGTTCGAGCTCTGACTGCAGACCTACCAAAACAGAGCATCTCATTCCCCGAGGGATTGAGGACTCC TGCCTCCCCATCTTCAGCGAATGCCTTTTACTCGCTCTCTCCCTCCACTCTGGTCCCCTCTGGCCTGCCA GAGTTTAAGGACAGCAGCAGCTCCCTCACAGATCCCCAGGTTTCCTACGTCAAGAGTCCAGCTGCAGAGA GGCGCAGTGGAGCAGTGGCTGGAGGCCCCGACACACCATCGGCCCAGCCCCTCGGGCCCCCCGCACTCCA GCCTGGTCCAGGCCTTTCCACGAAGAGTCCTCAGCCTTCTCCCTCCAGCCGGAAGAGCCCCCTGAGTCTG AGCCCTGCATTTCAGGTGCCTTTGGGCCCAGCTGAACAGGGCTCATCCCCACTCCTGAGCCCTGTCCTCA GTGATGCTGGCGGAGCCGGGATGGACTGCGAGGAGCCCAGACACAAGCGCGTGCCTGCAGACGAGGCCTA CTTCATAGTCAAAGAGATTCTCGCTACAGAACGAACATACCTCAAGGATTTAGAAGTTATTACCGTGTGG TTCCGCAGCGCAGTGGTGAAGGAGGACGCCATGCCTGCGACTCTGATGACGCTGCTCTTCTCCAACATCG ATCCCATCTATGAGTTCCACAGAGGCTTCCTGCGCGAGGTGGAGCAGAGGCTGGCACTCTGGGAAGGGCC CTCCAAAGCCCACACAAAAGGCAGTCATCAACGAATCGGGGACATCCTGCTCAGGAACATGCGCCAGTTA AAGGAGTTTACCAGCTACTTCCAAAGACATGACGAGGTCCTAACAGAACTGGAAAAGGCTACCAAACGCT GTAAGAAGTTGGAGGCAGTGTACAAGGAGTTTGAGCTGCAGAAGGTCTGCTACTTGCCTCTCAACACGTT CCTGCTGAAGCCCATCCAGCGGCTGCTGCACTACCGCCTGCTGCTGCGCCGCCTATGCGGACATTACAGC CCCGGGCACCATGACTACGCTGACTGCCATGACGCCCTGAAAGCCATCACAGAGGTGACCACCACACTAC AGCACATTCTCATCCGGCTGGAGAACCTGCAGAAGCTAACGGAGCTGCAGCGGGACCTGGTGGGCATAGA GAACCTCATTGCTCCTGGCAGGGAGTTCATCCGTGAGGGCTGCCTTCACAAGCTCACCAAGAAGGGCCTG CAGCAGAGGATGTTTTTTCTGTTCTCAGATATGTTGCTGTACACAAGCAAAGGAGTTGCAGGGACCAGCC ACTTCCGGATCCGGGGCCTCCTTCCCCTCCAAGGCATGCTGGTGGAAGAAAGTGATAACGAGTGGTCTGT TCCACACTGTTTCACCATCTACGCGGCTCAGAAAACAATCGTGGTGGCAGCCAGCACTCGGCTGGAGAAA GAGAAGTGGATGCTGGACCTGAACTCCGCGATCCAAGCAGCCAAGAGTGGCGGTGACACGGCCCCTGCAC TGCCAGGCCGCACTGTGTGCACTCGTCCCCCCAGATCCCCCAACGAGGTATCTCTGGAGCAGGAGTCAGA AGATGATGCTCGGGGTGTCCGCAGCTCCCTGGAGGGGCATGGCCAGCACCGGGCCAACACCACAATGCAC GTGTGCTGGTACCGGAACACCAGCGTGTCCAGGGCAGACCACAGTGCAGCTGTCGAGAACCAGCTTTCAG GATATCTGCTAAGAAAGTTCAAAAACAGTCATGGCTGGCAGAAGCTCTGGGTCGTCTTTACCAACTTCTG TTTGTTCTTCTACAAAACTCATCAGGATGACTACCCACTGGCCAGCCTCCCGCTGCTGGGCTACAGCGTG AGCATCCCCAGGGAGGCCGATGGCATACACAAAGACTATGTTTTCAAGCTCCAGTTCAAATCCCACGTCT ACTTCTTCCGGGCTGAGAGCAAGTACACATTTGAAAGGTGGATGGAGGTGATCCAGGGGGCCAGCAGCTC AGCCGGGAGGGCCCCAAGCATCGTGCAGGATGGCCCCCAACCCTCCTCAGGGCTGGAGGGGATGGTCAGG GGGAAGGAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216784 representing NM_014808
Red=Cloning site Green=Tags(s) MGEIEGTYRVLQTAGMRLGAQTPVGVSTLEPGQTLLPRMQEKHLHLRVKLLDNTMEIFDIEPKCDGQVLL TQVWKRLNLVECDYFGMEFQNTQSYWIWLEPMKPIIRQIRRPKNVVLRLAVKFFPPDPGQLQEEYTRYLF ALQLKRDLLEERLTCADTTAALLTSHLLQSEIGDYDETLDREHLKVNEYLPGQQHCLEKILEFHQKHVGQ TPAESDFQVLEIARKLEMYGIRFHMASDREGTKIQLAVSHMGVLVFQGTTKINTFNWSKVRKLSFKRKRF LIKLHPEVHGPYQDTLEFLLGSRDECKNFWKICVEYHTFFRLLDQPKPKAKAVFFSRGSSFRYSGRTQKQ LVDYFKDSGMKRIPYERRHSKTHTSVRALTADLPKQSISFPEGLRTPASPSSANAFYSLSPSTLVPSGLP EFKDSSSSLTDPQVSYVKSPAAERRSGAVAGGPDTPSAQPLGPPALQPGPGLSTKSPQPSPSSRKSPLSL SPAFQVPLGPAEQGSSPLLSPVLSDAGGAGMDCEEPRHKRVPADEAYFIVKEILATERTYLKDLEVITVW FRSAVVKEDAMPATLMTLLFSNIDPIYEFHRGFLREVEQRLALWEGPSKAHTKGSHQRIGDILLRNMRQL KEFTSYFQRHDEVLTELEKATKRCKKLEAVYKEFELQKVCYLPLNTFLLKPIQRLLHYRLLLRRLCGHYS PGHHDYADCHDALKAITEVTTTLQHILIRLENLQKLTELQRDLVGIENLIAPGREFIREGCLHKLTKKGL QQRMFFLFSDMLLYTSKGVAGTSHFRIRGLLPLQGMLVEESDNEWSVPHCFTIYAAQKTIVVAASTRLEK EKWMLDLNSAIQAAKSGGDTAPALPGRTVCTRPPRSPNEVSLEQESEDDARGVRSSLEGHGQHRANTTMH VCWYRNTSVSRADHSAAVENQLSGYLLRKFKNSHGWQKLWVVFTNFCLFFYKTHQDDYPLASLPLLGYSV SIPREADGIHKDYVFKLQFKSHVYFFRAESKYTFERWMEVIQGASSSAGRAPSIVQDGPQPSSGLEGMVR GKEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014808 |
| ORF Size | 3162 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014808.4 |
| RefSeq Size | 3997 bp |
| RefSeq ORF | 3165 bp |
| Locus ID | 9855 |
| UniProt ID | O94887 |
| Domains | RhoGEF, B41, PH |
| Protein Pathways | Adherens junction |
| MW | 119.7 kDa |
| Gene Summary | Functions as guanine nucleotide exchange factor that activates RAC1. May have relatively low activity. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. Plays a role in TNFSF11-mediated osteoclast differentiation, especially in podosome rearrangement and reorganization of the actin cytoskeleton. Regulates the activation of ITGB3, integrin signaling and cell adhesion (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Rho family GEF FARP2 is activated by aPKCι to control tight junction formation and polarity
,Elbediwy, A;Zhang, Y;Cobbaut, M;Riou, P;Tan, RS;Roberts, SK;Tynan, C;George, R;Kjaer, S;Martin-Fernandez, ML;Thompson, BJ;McDonald, NQ;Parker, PJ;,
J. Cell. Sci.
,PubMed ID 30872454
[FARP2]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216784L1 | Lenti-ORF clone of FARP2 (Myc-DDK-tagged)-Human FERM, RhoGEF and pleckstrin domain protein 2 (FARP2) |
CNY 9,736.00 |
|
| RC216784L2 | Lenti-ORF clone of FARP2 (mGFP-tagged)-Human FERM, RhoGEF and pleckstrin domain protein 2 (FARP2) |
CNY 12,450.00 |
|
| RC216784L3 | Lenti-ORF clone of FARP2 (Myc-DDK-tagged)-Human FERM, RhoGEF and pleckstrin domain protein 2 (FARP2) |
CNY 12,450.00 |
|
| RC216784L4 | Lenti-ORF clone of FARP2 (mGFP-tagged)-Human FERM, RhoGEF and pleckstrin domain protein 2 (FARP2) |
CNY 12,450.00 |
|
| RG216784 | FARP2 (tGFP-tagged) - Human FERM, RhoGEF and pleckstrin domain protein 2 (FARP2) |
CNY 11,590.00 |
|
| SC315123 | FARP2 (untagged)-Human FERM, RhoGEF and pleckstrin domain protein 2 (FARP2) |
CNY 7,344.00 |
