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MC3R (NM_019888) Human Tagged ORF Clone
CAT＃: RC215195
- TrueORF®
MC3R (Myc-DDK-tagged)-Human melanocortin 3 receptor (MC3R)
ORF Plasmid: tGFP
"NM_019888" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BMIQ9; MC3; MC3-R; OB20; OQTL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215195 representing NM_019888
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATCCAAAAGAAGTATCTGGAGGGAGATTTTGTCTTTCCTGTGAGCAGCAGCAGCTTCCTACGGA CCCTGCTGGAGCCCCAGCTCGGATCAGCCCTTCTGACAGCAATGAATGCTTCGTGCTGCCTGCCCTCTGT TCAGCCAACACTGCCTAATGGCTCGGAGCACCTCCAAGCCCCTTTCTTCAGCAACCAGAGCAGCAGCGCC TTCTGTGAGCAGGTCTTCATCAAGCCCGAGGTTTTCCTGTCTCTGGGCATCGTCAGTCTGCTGGAAAACA TCCTGGTTATCCTGGCCGTGGTCAGGAACGGCAACCTGCACTCCCCGATGTACTTCTTTCTCTGCAGCCT GGCGGTGGCCGACATGCTGGTAAGTGTGTCCAATGCCCTGGAGACCATCATGATCGCCATCGTCCACAGC GACTACCTGACCTTCGAGGACCAGTTTATCCAGCACATGGACAACATCTTCGACTCCATGATCTGCATCT CCCTGGTGGCCTCCATCTGCAACCTCCTGGCCATCGCCGTCGACAGGTACGTCACCATCTTTTACGCGCT CCGCTACCACAGCATCATGACCGTGAGGAAGGCCCTCACCTTGATCGTGGCCATCTGGGTCTGCTGCGGC GTCTGTGGCGTGGTGTTCATCGTCTACTCGGAGAGCAAAATGGTCATTGTGTGCCTCATCACCATGTTCT TCGCCATGATGCTCCTCATGGGCACCCTCTACGTGCACATGTTCCTCTTTGCGCGGCTGCACGTCAAGCG CATAGCAGCACTGCCACCTGCCGACGGGGTGGCCCCACAGCAACACTCATGCATGAAGGGGGCAGTCACC ATCACCATTCTCCTGGGCGTGTTCATCTTCTGCTGGGCCCCCTTCTTCCTCCACCTGGTCCTCATCATCA CCTGCCCCACCAACCCCTACTGCATCTGCTACACTGCCCACTTCAACACCTACCTGGTCCTCATCATGTG CAACTCCGTCATCGACCCACTCATCTACGCTTTCCGGAGCCTGGAATTGCGCAACACCTTTAGGGAGATT CTCTGTGGCTGCAACGGCATGAACTTGGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215195 representing NM_019888
Red=Cloning site Green=Tags(s) MSIQKKYLEGDFVFPVSSSSFLRTLLEPQLGSALLTAMNASCCLPSVQPTLPNGSEHLQAPFFSNQSSSA FCEQVFIKPEVFLSLGIVSLLENILVILAVVRNGNLHSPMYFFLCSLAVADMLVSVSNALETIMIAIVHS DYLTFEDQFIQHMDNIFDSMICISLVASICNLLAIAVDRYVTIFYALRYHSIMTVRKALTLIVAIWVCCG VCGVVFIVYSESKMVIVCLITMFFAMMLLMGTLYVHMFLFARLHVKRIAALPPADGVAPQQHSCMKGAVT ITILLGVFIFCWAPFFLHLVLIITCPTNPYCICYTAHFNTYLVLIMCNSVIDPLIYAFRSLELRNTFREI LCGCNGMNLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019888 |
| ORF Size | 1080 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019888.2, NP_063941.2 |
| RefSeq Size | 1083 bp |
| RefSeq ORF | 972 bp |
| Locus ID | 4159 |
| UniProt ID | P41968 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 35.9 kDa |
| Gene Summary | This gene encodes a G-protein-coupled receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone that is expressed in tissues other than the adrenal cortex and melanocytes. This gene maps to the same region as the locus for benign neonatal epilepsy. Mice deficient for this gene have increased fat mass despite decreased food intake, suggesting a role for this gene product in the regulation of energy homeostasis. Mutations in this gene are associated with a susceptibility to obesity in humans. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Therapeutic senescence via GPCR activation in synovial fibroblasts facilitates resolution of arthritis
,Montero-Melendez, T;Nagano, A;Chelala, C;Filer, A;Buckley, CD;Perretti, M;,
Nat Commun
,PubMed ID 32029712
[MC3R]
|
|
Ligand Bias and Its Association With Pro-resolving Actions of Melanocortin Drugs
,Patruno, S;Garrido-Mesa, J;Romano, M;Perretti, M;Montero-Melendez, T;,
Front Pharmacol
,PubMed ID 30154720
[MC3R]
|
|
MC4R-dependent suppression of appetite by bone-derived lipocalin 2
,Mosialou, I;Shikhel, S;Liu, JM;Maurizi, A;Luo, N;He, Z;Huang, Y;Zong, H;Friedman, RA;Barasch, J;Lanzano, P;Deng, L;Leibel, RL;Rubin, M;Nicholas, T;Chung, W;Zeltser, LM;Williams, KW;Pessin, JE;Kousteni, S;,
Nature
,PubMed ID 28273060
[MC3R]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215195L1 | Lenti ORF clone of Human melanocortin 3 receptor (MC3R), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215195L2 | Lenti ORF clone of Human melanocortin 3 receptor (MC3R), mGFP tagged |
CNY 5,890.00 |
|
| RC215195L3 | Lenti ORF clone of Human melanocortin 3 receptor (MC3R), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215195L4 | Lenti ORF clone of Human melanocortin 3 receptor (MC3R), mGFP tagged |
CNY 5,890.00 |
|
| RG215195 | MC3R (tGFP-tagged) - Human melanocortin 3 receptor (MC3R) |
CNY 4,370.00 |
|
| SC113222 | MC3R (untagged)-Human melanocortin 3 receptor (MC3R) |
CNY 2,950.00 |
