DAB2IP (NM_032552) Human Tagged ORF Clone

CAT#: RC214413

DAB2IP (Myc-DDK-tagged)-Human DAB2 interacting protein (DAB2IP), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_032552" in other vectors (6)

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CNY 11,824.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-DAB2IP Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AF9Q34; AIP-1; AIP1; DIP1/2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214413 representing NM_032552
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCGACTCCCTTCTGGACCAAGACGACTCCTACGAGTCGCCTCAAGAAAGGCCGGGCTCTCGGC
GCAGCCTGCCTGGCAGCCTTTCCGAGAAGAGCCCCAGCATGGAGCCCTCGGCCGCCACGCCGTTCCGGGT
CACGGGCTTCCTCAGCCGCCGCCTCAAGGGCTCCATCAAGCGCACCAAGAGCCAGCCCAAGCTGGACCGC
AACCACAGCTTCCGCCACATCCTGCCGGGGTTCCGGAGCGCCGCCGCCGCCGCCGCGGACAATGAGAGGT
CCCATCTGATGCCGAGGCTGAAGGAGTCTCGCTCCCACGAGTCCCTGCTCAGCCCCAGCAGTGCGGTGGA
GGCGCTGGACCTCAGCATGGAGGAAGAGGTGGTCATCAAGCCCGTGCACAGCAGCATCCTTGGCCAGGAC
TACTGCTTCGAGGTGACGACGTCATCAGGAAGCAAGTGCTTTTCCTGCCGGTCTGCAGCTGAGCGGGATA
AGTGGATGGAGAACCTCCGGCGAGCGGTGCATCCCAACAAGGACAACAGCCGGCGTGTGGAGCACATCCT
GAAGCTGTGGGTGATCGAGGCCAAGGACCTGCCAGCCAAGAAGAAGTACCTGTGCGAGCTGTGCCTGGAC
GATGTGCTCTATGCCCGCACCACGGGCAAGCTCAAGACGGACAATGTTTTCTGGGGCGAGCACTTCGAGT
TCCACAACTTGCCGCCTCTGCGCACGGTCACTGTCCACCTGTACCGGGAGACCGACAAGAAGAAGAAGAA
GGAGCGCAACAGTTACCTGGGCCTGGTGAGCCTACCTGCTGCCTCGGTGGCCGGGCGGCAGTTCGTGGAG
AAGTGGTACCCGGTGGTGACGCCCAACCCCAAGGGCGGCAAGGGCCCTGGACCCATGATCCGCATCAAGG
CGCGCTACCAAACCATCACCATCCTGCCCATGGAGATGTACAAAGAGTTCGCTGAGCACATCACCAACCA
CTACCTGGGGCTGTGTGCAGCCCTCGAGCCCATCCTCAGTGCCAAGACCAAGGAGGAGATGGCATCTGCC
CTGGTGCACATCCTGCAGAGCACGGGCAAGGTGAAGGACTTCCTGACAGACCTGATGATGTCAGAGGTGG
ACCGCTGCGGGGACAACGAGCACCTCATCTTCCGGGAGAACACACTGGCCACCAAGGCCATTGAGGAGTA
CCTCAAGCTAGTGGGCCAGAAGTACCTGCAGGACGCCCTAGGTGAGTTCATCAAAGCGCTGTATGAGTCA
GATGAGAACTGCGAAGTGGATCCCAGCAAGTGCTCGGCCGCTGACCTCCCAGAGCACCAGGGCAACCTCA
AGATGTGCTGCGAGCTGGCCTTCTGCAAGATCATCAACTCCTACTGTGTCTTCCCACGGGAGTTGAAAGA
GGTGTTTGCCTCGTGGAGGCAGGAGTGCAGCAGTCGCGGCCGCCCGGACATCAGTGAGCGGCTCATCAGC
GCCTCCCTCTTCCTGCGCTTCCTCTGCCCAGCCATCATGTCGCCCTCACTCTTCAACCTGCTGCAGGAGT
ACCCTGATGACCGCACTGCCCGCACCCTCACCCTCATCGCCAAGGTCACCCAGAACCTGGCCAACTTTGC
CAAATTTGGCAGCAAGGAGGAATACATGTCCTTCATGAACCAGTTCCTAGAGCATGAGTGGACCAACATG
CAGCGCTTCCTGCTGGAGATCTCCAACCCCGAGACCCTCTCCAATACAGCCGGCTTCGAGGGCTACATCG
ACCTGGGCCGCGAGCTCTCCAGCCTGCACTCACTGCTCTGGGAGGCCGTCAGCCAGCTGGAGCAGAGCAT
AGTATCCAAACTGGGACCCCTGCCTCGGATCCTGAGGGACGTCCACACAGCACTGAGCACCCCAGGTAGC
GGGCAGCTCCCAGGGACCAATGACCTGGCCTCCACACCGGGCTCTGGCAGCAGCAGCATCTCAGCTGGGC
TGCAGAAGATGGTGATTGAGAACGATCTTTCCGGTCTGATAGATTTCACCCGGTTACCGTCTCCAACCCC
CGAAAACAAGGACTTGTTTTTTGTCACAAGGTCCTCCGGGGTCCAGCCCTCACCTGCCCGCAGCTCGAGT
TACTCGGAAGCCAACGAGCCTGATCTTCAGATGGCCAACGGTGGCAAGAGCCTCTCCATGGTGGACCTCC
AGGACGCCCGCACGCTGGATGGGGAGGCAGGCTCCCCGGCGGGCCCCGACGTCCTCCCCACAGATGGGCA
GGCCGCTGCAGCTCAGCTGGTGGCCGGGTGGCCGGCCCGGGCAACCCCAGTGAACCTGGCAGGGCTGGCC
ACGGTGCGGCGGGCAGGCCAGACACCAACCACACCAGGCACCTCCGAGGGCGCGCCAGGCCGGCCCCAGC
TGTTGGCACCGCTCTCCTTCCAGAACCCTGTGTACCAGATGGCGGCTGGCCTGCCGCTGTCACCCCGTGG
CCTTGGCGACTCAGGCTCTGAGGGCCACAGCTCCCTGAGCTCACACAGCAACAGCGAGGAGTTGGCGGCT
GCTGCCAAGCTGGGAAGTTTCAGCACTGCCGCGGAGGAGCTGGCTCGGCGGCCCGGTGAGCTGGCACGGC
GACAGATGTCACTGACTGAAAAAGGCGGGCAGCCCACGGTGCCACGGCAGAACAGTGCTGGCCCCCAGAG
GAGGATCGACCAGCCTCCGCCCCCACCCCCGCCGCCACCTCCTGCCCCCCGCGGCCGGACGCCCCCCAAC
CTGCTGAGCACCCTGCAGTACCCAAGACCCTCAAGCGGAACCCTGGCGTCGGCCTCACCTGATTGGGTGG
GCCCCAGTACCCGCCTGAGGCAGCAGTCCTCTTCCTCCAAGGGGGACAGCCCAGAACTGAAGCCACGGGC
AGTGCACAAGCAGGGCCCTTCACCTGTGAGCCCCAATGCCCTGGACCGCACAGCCGCTTGGCTCTTGACC
ATGAACGCGCAGTTGTTAGAAGACGAGGGCCTGGGCCCAGACCCCCCCCACAGGGATAGGCTAAGGAGTA
AGGACGAGCTCAGCCAAGCAGAAAAGGACCTGGCGGTGCTGCAGGACAAGCTGCGAATCTCCACCAAGAA
GCTGGAGGAGTATGAGACCCTGTTCAAGTGCCAGGAGGAGACGACGCAGAAGCTGGTGCTGGAGTACCAG
GCACGGCTGGAGGAGGGCGAGGAGCGGCTGCGGCGGCAGCAGGAGGACAAGGACATCCAGATGAAGGGCA
TCATCAGCAGGTTGATGTCCGTGGAGGAAGAACTGAAGAAGGACCACGCAGAGATGCAAGCGGCTGTGGA
CTCCAAACAGAAGATCATTGATGCCCAGGAGAAGCGCATTGCCTCGTTGGATGCCGCCAATGCCCGCCTC
ATGAGTGCCCTGACCCAGCTGAAAGAGAGTATGCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214413 representing NM_032552
Red=Cloning site Green=Tags(s)

MEPDSLLDQDDSYESPQERPGSRRSLPGSLSEKSPSMEPSAATPFRVTGFLSRRLKGSIKRTKSQPKLDR
NHSFRHILPGFRSAAAAAADNERSHLMPRLKESRSHESLLSPSSAVEALDLSMEEEVVIKPVHSSILGQD
YCFEVTTSSGSKCFSCRSAAERDKWMENLRRAVHPNKDNSRRVEHILKLWVIEAKDLPAKKKYLCELCLD
DVLYARTTGKLKTDNVFWGEHFEFHNLPPLRTVTVHLYRETDKKKKKERNSYLGLVSLPAASVAGRQFVE
KWYPVVTPNPKGGKGPGPMIRIKARYQTITILPMEMYKEFAEHITNHYLGLCAALEPILSAKTKEEMASA
LVHILQSTGKVKDFLTDLMMSEVDRCGDNEHLIFRENTLATKAIEEYLKLVGQKYLQDALGEFIKALYES
DENCEVDPSKCSAADLPEHQGNLKMCCELAFCKIINSYCVFPRELKEVFASWRQECSSRGRPDISERLIS
ASLFLRFLCPAIMSPSLFNLLQEYPDDRTARTLTLIAKVTQNLANFAKFGSKEEYMSFMNQFLEHEWTNM
QRFLLEISNPETLSNTAGFEGYIDLGRELSSLHSLLWEAVSQLEQSIVSKLGPLPRILRDVHTALSTPGS
GQLPGTNDLASTPGSGSSSISAGLQKMVIENDLSGLIDFTRLPSPTPENKDLFFVTRSSGVQPSPARSSS
YSEANEPDLQMANGGKSLSMVDLQDARTLDGEAGSPAGPDVLPTDGQAAAAQLVAGWPARATPVNLAGLA
TVRRAGQTPTTPGTSEGAPGRPQLLAPLSFQNPVYQMAAGLPLSPRGLGDSGSEGHSSLSSHSNSEELAA
AAKLGSFSTAAEELARRPGELARRQMSLTEKGGQPTVPRQNSAGPQRRIDQPPPPPPPPPPAPRGRTPPN
LLSTLQYPRPSSGTLASASPDWVGPSTRLRQQSSSSKGDSPELKPRAVHKQGPSPVSPNALDRTAAWLLT
MNAQLLEDEGLGPDPPHRDRLRSKDELSQAEKDLAVLQDKLRISTKKLEEYETLFKCQEETTQKLVLEYQ
ARLEEGEERLRRQQEDKDIQMKGIISRLMSVEEELKKDHAEMQAAVDSKQKIIDAQEKRIASLDAANARL
MSALTQLKESMH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032552
ORF Size 3396 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032552.3, NP_115941.2
RefSeq Size 5472 bp
RefSeq ORF 3399 bp
Locus ID 153090
UniProt ID Q5VWQ8
MW 124.9 kDa
Gene Summary DAB2IP is a Ras (MIM 190020) GTPase-activating protein (GAP) that acts as a tumor suppressor. The DAB2IP gene is inactivated by methylation in prostate and breast cancers (Yano et al., 2005 [PubMed 15386433]).[supplied by OMIM, May 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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