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MTR (NM_000254) Human Tagged ORF Clone
CAT＃: RC214275
- TrueORF®
MTR (Myc-DDK-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR)
ORF Plasmid: tGFP
"NM_000254" in other vectors (6)
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CNY 14,088.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | cblG; HMAG; MS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214275 representing NM_000254
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACCCGCGCTCCAAGACCTGTCGCAACCCGAAGGTCTGAAGAAAACCCTGCGGGATGAGATCAATG CCATTCTGCAGAAGAGGATTATGGTGCTGGATGGAGGGATGGGGACCATGATCCAGCGGGAGAAGCTAAA CGAAGAACACTTCCGAGGTCAGGAATTTAAAGATCATGCCAGGCCGCTGAAAGGCAACAATGACATTTTA AGTATAACTCAGCCTGATGTCATTTACCAAATCCATAAGGAATACTTGCTGGCTGGGGCAGATATCATTG AAACAAATACTTTTAGCAGCACTAGTATTGCCCAAGCTGACTATGGCCTTGAACACTTGGCCTACCGGAT GAACATGTGCTCTGCAGGAGTGGCCAGAAAAGCTGCCGAGGAGGTAACTCTCCAGACAGGAATTAAGAGG TTTGTGGCAGGGGCTCTGGGTCCGACTAATAAGACACTCTCTGTGTCCCCATCTGTGGAAAGGCCGGATT ATAGGAACATCACATTTGATGAGCTTGTTGAAGCATACCAAGAGCAGGCCAAAGGACTTCTGGATGGCGG GGTTGATATCTTACTCATTGAAACTATTTTTGATACTGCCAATGCCAAGGCAGCCTTGTTTGCACTCCAA AATCTTTTTGAGGAGAAATATGCTCCCCGGCCTATCTTTATTTCAGGGACGATCGTTGATAAAAGTGGGC GGACTCTTTCCGGACAGACAGGAGAGGGATTTGTCATCAGCGTGTCTCATGGAGAACCACTCTGCATTGG ATTAAATTGTGCTTTGGGTGCAGCTGAAATGAGACCTTTTATTGAAATAATTGGAAAATGTACAACAGCC TATGTCCTCTGTTATCCCAATGCAGGTCTTCCCAACACCTTTGGTGACTATGATGAAACGCCTTCTATGA TGGCCAAGCACCTAAAGGATTTTGCTATGGATGGCTTGGTCAATATAGTTGGAGGATGCTGTGGGTCAAC ACCAGATCATATCAGGGAAATTGCTGAAGCTGTGAAAAATTGTAAGCCTAGAGTTCCACCTGCCACTGCT TTTGAAGGACATATGTTACTGTCTGGTCTAGAGCCCTTCAGGATTGGACCGTACACCAACTTTGTTAACA TTGGAGAGCGCTGTAATGTTGCAGGATCAAGGAAGTTTGCTAAACTCATCATGGCAGGAAACTATGAAGA AGCCTTGTGTGTTGCCAAAGTGCAGGTGGAAATGGGAGCCCAGGTGTTGGATGTCAACATGGATGATGGC ATGCTAGATGGTCCAAGTGCAATGACCAGATTTTGCAACTTAATTGCTTCCGAGCCAGACATCGCAAAGG TACCTTTGTGCATCGACTCCTCCAATTTTGCTGTGATTGAAGCTGGGTTAAAGTGCTGCCAAGGGAAGTG CATTGTCAATAGCATTAGTCTGAAGGAAGGAGAGGACGACTTCTTGGAGAAGGCCAGGAAGATTAAAAAG TATGGAGCTGCTATGGTGGTCATGGCTTTTGATGAAGAAGGACAGGCAACAGAAACAGACACAAAAATCA GAGTGTGCACCCGGGCCTACCATCTGCTTGTGAAAAAACTGGGCTTTAATCCAAATGACATTATTTTTGA CCCTAATATCCTAACCATTGGGACTGGAATGGAGGAACACAACTTGTATGCCATTAATTTTATCCATGCA ACAAAAGTCATTAAAGAAACATTACCTGGAGCCAGAATAAGTGGAGGTCTTTCCAACTTGTCCTTCTCCT TCCGAGGAATGGAAGCCATTCGAGAAGCAATGCATGGGGTTTTCCTTTACCATGCAATCAAGTCTGGCAT GGACATGGGGATAGTGAATGCTGGAAACCTCCCTGTGTATGATGATATCCATAAGGAACTTCTGCAGCTC TGTGAAGATCTCATCTGGAATAAAGACCCTGAGGCCACTGAGAAGCTCTTACGTTATGCCCAGACTCAAG GCACAGGAGGGAAGAAAGTCATTCAGACTGATGAGTGGAGAAATGGCCCTGTCGAAGAACGCCTTGAGTA TGCCCTTGTGAAGGGCATTGAAAAACATATTATTGAGGATACTGAGGAAGCCAGGTTAAACCAAAAAAAA TATCCCCGACCTCTCAATATAATTGAAGGACCCCTGATGAATGGAATGAAAATTGTTGGTGATCTTTTTG GAGCTGGAAAAATGTTTCTACCTCAGGTTATAAAGTCAGCCCGGGTTATGAAGAAGGCTGTTGGCCACCT TATCCCTTTCATGGAAAAAGAAAGAGAAGAAACCAGAGTGCTTAACGGCACAGTAGAAGAAGAGGACCCT TACCAGGGCACCATCGTGCTGGCCACTGTTAAAGGCGACGTGCACGACATAGGCAAGAACATAGTTGGAG TAGTCCTTGGCTGCAATAATTTCCGAGTTATTGATTTAGGAGTCATGACTCCATGTGATAAGATACTGAA AGCTGCTCTTGACCACAAAGCAGATATAATTGGCCTGTCAGGACTCATCACTCCTTCCCTGGATGAAATG ATTTTTGTTGCCAAGGAAATGGAGAGATTAGCTATAAGGATTCCATTGTTGATTGGAGGAGCAACCACTT CAAAAACCCACACAGCAGTTAAAATAGCTCCGAGATACAGTGCACCTGTAATCCATGTCCTGGACGCGTC CAAGAGTGTGGTGGTGTGTTCCCAGCTGTTAGATGAAAATCTAAAGGATGAATACTTTGAGGAAATCATG GAAGAATATGAAGATATTAGACAGGACCATTATGAGTCTCTCAAGGAGAGGAGATACTTACCCTTAAGTC AAGCCAGAAAAAGTGGTTTCCAAATGGATTGGCTGTCTGAACCTCACCCAGTGAAGCCCACGTTTATTGG GACCCAGGTCTTTGAAGACTATGACCTGCAGAAGCTGGTGGACTACATTGACTGGAAGCCTTTCTTTGAT GTCTGGCAGCTCCGGGGCAAGTACCCGAATCGAGGCTTTCCCAAGATATTTAACGACAAAACAGTAGGTG GAGAGGCCAGGAAGGTCTACGATGATGCCCACAATATGCTGAACACACTGATTAGTCAAAAGAAACTCCG GGCCCGGGGTGTGGTTGGGTTCTGGCCAGCACAGAGTATCCAAGACGACATTCACCTGTACGCAGAGGCT GCTGTGCCCCAGGCTGCAGAGCCCATAGCCACCTTCTATGGGTTAAGGCAACAGGCTGAGAAGGACTCTG CCAGCACGGAGCCATACTACTGCCTCTCAGACTTCATCGCTCCCTTGCATTCTGGCATCCGTGACTACCT GGGCCTGTTTGCCGTTGCCTGCTTTGGGGTAGAAGAGCTGAGCAAGGCCTATGAGGATGATGGTGACGAC TACAGCAGCATCATGGTCAAGGCGCTGGGGGACCGGCTGGCAGAGGCCTTTGCAGAAGAGCTCCATGAAA GAGTTCGCCGAGAACTGTGGGCCTACTGTGGCAGTGAGCAGCTGGACGTCGCAGACCTGCGCAGGCTGCG GTACAAGGGCATCCGCCCGGCTCCTGGCTACCCCAGCCAGCCCGACCACACCGAGAAGCTCACCATGTGG AGACTCGCAGACATCGAGCAGTCTACAGGCATTAGGTTAACAGAATCATTAGCAATGGCACCTGCTTCAG CAGTCTCAGGCCTCTACTTCTCCAATTTGAAGTCCAAATATTTTGCTGTGGGGAAGATTTCCAAGGATCA GGTTGAGGATTATGCATTGAGGAAGAACATATCTGTGGCTGAGGTTGAGAAATGGCTTGGACCCATTTTG GGATATGATACAGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC214275 representing NM_000254
Red=Cloning site Green=Tags(s) MSPALQDLSQPEGLKKTLRDEINAILQKRIMVLDGGMGTMIQREKLNEEHFRGQEFKDHARPLKGNNDIL SITQPDVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEEVTLQTGIKR FVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYQEQAKGLLDGGVDILLIETIFDTANAKAALFALQ NLFEEKYAPRPIFISGTIVDKSGRTLSGQTGEGFVISVSHGEPLCIGLNCALGAAEMRPFIEIIGKCTTA YVLCYPNAGLPNTFGDYDETPSMMAKHLKDFAMDGLVNIVGGCCGSTPDHIREIAEAVKNCKPRVPPATA FEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALCVAKVQVEMGAQVLDVNMDDG MLDGPSAMTRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKK YGAAMVVMAFDEEGQATETDTKIRVCTRAYHLLVKKLGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHA TKVIKETLPGARISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKSGMDMGIVNAGNLPVYDDIHKELLQL CEDLIWNKDPEATEKLLRYAQTQGTGGKKVIQTDEWRNGPVEERLEYALVKGIEKHIIEDTEEARLNQKK YPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETRVLNGTVEEEDP YQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEM IFVAKEMERLAIRIPLLIGGATTSKTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEIM EEYEDIRQDHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVFEDYDLQKLVDYIDWKPFFD VWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGVVGFWPAQSIQDDIHLYAEA AVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFAVACFGVEELSKAYEDDGDD YSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGIRPAPGYPSQPDHTEKLTMW RLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDYALRKNISVAEVEKWLGPIL GYDTD SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000254 |
| ORF Size | 3795 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000254.3 |
| RefSeq Size | 7122 bp |
| RefSeq ORF | 3798 bp |
| Locus ID | 4548 |
| UniProt ID | Q99707 |
| Domains | Pterin_bind, S-methyl_trans, B12-binding_2, Met_synt_B12, B12-binding |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways, One carbon pool by folate |
| MW | 140.3 kDa |
| Gene Summary | This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214275L1 | Lenti-ORF clone of MTR (Myc-DDK-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 14,540.00 |
|
| RC214275L2 | Lenti-ORF clone of MTR (mGFP-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 14,540.00 |
|
| RC214275L3 | Lenti-ORF clone of MTR (Myc-DDK-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 14,540.00 |
|
| RC214275L4 | Lenti-ORF clone of MTR (mGFP-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 14,540.00 |
|
| RG214275 | MTR (tGFP-tagged) - Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 13,870.00 |
|
| SC309934 | MTR (untagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 13,224.00 |
