PGAM4 (NM_001029891) Human Tagged ORF Clone

CAT#: RC213480

PGAM4 (Myc-DDK-tagged)-Human phosphoglycerate mutase family member 4 (PGAM4)

ORF Plasmid: DDK tGFP



  "NM_001029891" in other vectors (4)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PGAM4 mouse monoclonal antibody,clone OTI2A12
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms dJ1000K24.1; PGAM-B; PGAM1; PGAM3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213480 representing NM_001029891
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCTACAAACTGGTGCTGATCCGGCACGGCGAGAGCACATGGAACCTGGAGAACCGCTTCAGCT
GCTGGTACGACGCCGATCTGAGCCCGGCGGGCCACGAGGAGGCGAAGCGCGGCGGGCAGGCGCTACGAGA
TGCTGGCTATGAGTTTGACATCTGCCTCACCTCAGTGCAGAAGAGAGTGATCCGGACCCTCTGGACAGTG
CTAGATGCCATTGATCAGATGTGGCTGCCAGTGGTGAGGACTTGGCGCCTCAATGAGCGGCACTATGGGG
GTCTAACCGGTCTCAATAAAGCAGAAACTGCTGCAAAGCATGGTGAGGCCCAGGTGAAGATCTGGAGGCG
CTCCTATGATGTCCCACCACCTCCGATGGAGCCCGACCATCCTTTCTACAGCAACATCAGTAAGGATCGC
AGGTATGCAGACCTCACAGAAGATCAGCTACCCTCCTATGAGAGTCCGAAGGATACTATTGCCAGAGCTC
TGCCCTTCTGGAATGAAGAAATAGTTCCCCAGATCAAGGAGGGGAAACGTGTACTGATTGCAGCCCATGG
CAACAGCCTCCAGGGCATTGCCAAGCATGTGGAGGGTCTCTCTGAAGAGGCTATCATGGAGCTGAACCTG
CCGACTGGTATTCCCATCGTCTATGAATTGGACAAGAACTTGAAGCCTATCAAGCCCATGCAGTTTCTGG
GGGATGAAGAGACGGTGTGCAAAGCCATAGAAGCTGTGGCTGCCCAGGGCAAGGCCAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213480 representing NM_001029891
Red=Cloning site Green=Tags(s)

MAAYKLVLIRHGESTWNLENRFSCWYDADLSPAGHEEAKRGGQALRDAGYEFDICLTSVQKRVIRTLWTV
LDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDR
RYADLTEDQLPSYESPKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLQGIAKHVEGLSEEAIMELNL
PTGIPIVYELDKNLKPIKPMQFLGDEETVCKAIEAVAAQGKAKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001029891
ORF Size 762 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001029891.3
RefSeq Size 1678 bp
RefSeq ORF 765 bp
Locus ID 441531
UniProt ID Q8N0Y7
Protein Pathways Glycolysis / Gluconeogenesis, Metabolic pathways
MW 28.6 kDa
Gene Summary This intronless gene appears to have arisen from a retrotransposition event, yet it is thought to be an expressed, protein-coding gene. The encoded protein is a member of the phosphoglycerate mutase family, a set of enzymes that catalyze the transfer of a phosphate group from 3-phosphoglycerate to 2-phosphoglycerate. [provided by RefSeq, May 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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