NMBR (NM_002511) Human Tagged ORF Clone

CAT#: RC213319

NMBR (Myc-DDK-tagged)-Human neuromedin B receptor (NMBR)

ORF Plasmid: DDK tGFP



  "NM_002511" in other vectors (6)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-NMBR Antibody (Cytoplasmic Domain)
    • 50 ug

CNY 5,654.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BB1; BB1R; BRS1; NMB-R
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213319 representing NM_002511
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTCTAAGTCTCTTTCCAACCTCTCGGTGACCACCGGCGCGAATGAGAGCGGTTCCGTTCCCGAGG
GGTGGGAAAGGGATTTCCTGCCGGCCTCGGACGGGACCACCACGGAGTTGGTGATCCGCTGTGTGATCCC
GTCCCTCTACCTGCTCATCATCACCGTGGGCTTGCTGGGCAACATCATGCTGGTGAAGATCTTCATCACC
AACAGCGCCATGAGGAGCGTCCCCAACATCTTCATCTCTAACCTGGCGGCCGGGGACTTGCTGCTGCTGC
TCACCTGCGTCCCGGTGGACGCCTCGCGCTACTTCTTCGACGAGTGGATGTTTGGCAAGGTGGGCTGCAA
ACTGATCCCTGTCATCCAGCTCACTTCCGTGGGGGTTTCCGTGTTCACTCTCACTGCCCTCAGCGCCGAC
AGGTACAGAGCCATCGTTAACCCCATGGACATGCAGACGTCAGGGGCATTGCTGCGGACCTGTGTGAAGG
CCATGGGTATCTGGGTGGTCTCCGTGTTGCTGGCAGTTCCCGAAGCGGTGTTTTCAGAAGTGGCTCGCAT
CAGTAGCTTGGATAATAGCAGCTTCACAGCATGTATCCCATACCCTCAAACAGATGAATTACATCCAAAG
ATTCATTCAGTGCTCATTTTCTTGGTCTATTTCCTCATACCACTTGCCATTATTAGCATTTATTATTGTC
ATATTGCAAAGACCTTAATTAAAAGCGCACACAATCTTCCTGGAGAATACAATGAACATACCAAAAAACA
GATGGAAACACGGAAACGCCTGGCTAAAATTGTGCTTGTCTTTGTGGGCTGTTTCATCTTCTGTTGGTTT
CCAAACCACATCCTTTACATGTATCGGTCTTTCAACTATAATGAGATTGATCCATCTCTAGGCCACATGA
TTGTCACCTTAGTTGCCCGGGTTCTCAGTTTTGGCAATTCTTGTGTCAACCCATTTGCTCTTTACCTACT
CAGTGAAAGCTTCAGGAGGCATTTCAACAGCCAACTCTGCTGTGGGAGGAAGTCCTATCAAGAGAGAGGA
ACCAGCTACCTACTCAGCTCTTCAGCGGTGCGTATGACATCTCTGAAAAGCAATGCTAAGAACATGGTGA
CCAATTCTGTTTTACTAAATGGGCACAGCATGAAGCAGGAAATGGCAATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213319 representing NM_002511
Red=Cloning site Green=Tags(s)

MPSKSLSNLSVTTGANESGSVPEGWERDFLPASDGTTTELVIRCVIPSLYLLIITVGLLGNIMLVKIFIT
NSAMRSVPNIFISNLAAGDLLLLLTCVPVDASRYFFDEWMFGKVGCKLIPVIQLTSVGVSVFTLTALSAD
RYRAIVNPMDMQTSGALLRTCVKAMGIWVVSVLLAVPEAVFSEVARISSLDNSSFTACIPYPQTDELHPK
IHSVLIFLVYFLIPLAIISIYYCHIAKTLIKSAHNLPGEYNEHTKKQMETRKRLAKIVLVFVGCFIFCWF
PNHILYMYRSFNYNEIDPSLGHMIVTLVARVLSFGNSCVNPFALYLLSESFRRHFNSQLCCGRKSYQERG
TSYLLSSSAVRMTSLKSNAKNMVTNSVLLNGHSMKQEMAM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002511
ORF Size 1170 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002511.4
RefSeq Size 1352 bp
RefSeq ORF 1173 bp
Locus ID 4829
UniProt ID P28336
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 43.3 kDa
Gene Summary This gene encodes a 7-transmembrane G protein-coupled receptor that binds neuromedin B, which is a growth factor and mitogen for gastrointestinal epithelial tissue and for normal and neoplastic lung. This receptor may play a role in smooth muscle contraction, neuronal responses, and the regulation of cell growth. Antagonists of this receptor have a potential therapeutic use in inhibiting tumor cell growth. Polymorphisms in this gene may be associated with a susceptibility for schizophrenia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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