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IRS2 (NM_003749) Human Tagged ORF Clone
CAT＃: RC212413
IRS2 (Myc-DDK-tagged)-Human insulin receptor substrate 2 (IRS2)
ORF Plasmid: tGFP
"NM_003749" in other vectors (6)
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CNY 13,728.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IRS-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212413 representing NM_003749.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGAGCCCGCCGCGGCACGGGCCGCCCGGGCCGGCGAGCGGAGACGGCCCCAACCTCAACAACAAC AACAACAACAACAACCACAGCGTGCGCAAGTGCGGCTACCTGCGCAAGCAGAAGCATGGCCACAAGCGC TTCTTCGTGCTGCGCGGACCCGGCGCGGGCGGCGACGAGGCGACGGCGGGCGGGGGGTCGGCGCCGCAA CCGCCGCGGCTCGAGTACTACGAGAGCGAGAAAAAGTGGCGGAGCAAGGCAGGCGCGCCGAAACGGGTG ATCGCTCTCGACTGCTGCCTGAACATCAACAAGCGCGCCGACGCCAAGCACAAGTACCTGATCGCCCTC TACACCAAGGACGAGTACTTCGCCGTGGCCGCCGAGAACGAGCAGGAGCAGGAGGGCTGGTACCGCGCG CTCACCGACCTGGTCAGCGAGGGCCGCGCGGCCGCCGGAGACGCGCCCCCCGCCGCCGCGCCCGCCGCG TCCTGCAGCGCCTCCCTGCCCGGCGCCCTGGGCGGCTCTGCCGGCGCCGCCGGGGCCGAGGACAGCTAC GGGCTGGTGGCTCCCGCCACGGCCGCCTACCGTGAGGTGTGGCAGGTGAACCTGAAGCCCAAGGGTCTG GGCCAGAGCAAGAACCTGACGGGGGTGTACCGTCTGTGCCTGTCTGCGCGCACCATCGGCTTCGTGAAG CTCAACTGCGAGCAGCCGTCGGTGACGCTGCAGCTCATGAACATCCGCCGCTGCGGCCACTCGGACAGC TTCTTCTTCATCGAGGTGGGCCGCTCGGCCGTCACAGGCCCCGGCGAGCTGTGGATGCAGGCGGACGAC TCGGTGGTGGCGCAGAACATCCACGAGACCATCCTGGAGGCCATGAAGGCGCTCAAGGAGCTCTTCGAG TTCCGGCCGCGCAGTAAGAGCCAATCGTCGGGGTCGTCGGCCACGCACCCCATCAGCGTCCCCGGCGCG CGCCGCCACCACCACCTGGTCAACCTGCCCCCCAGCCAGACGGGCCTGGTGCGCCGCTCGCGCACCGAC AGCCTGGCCGCCACCCCGCCGGCGGCCAAGTGCAGCTCGTGCCGGGTGCGCACCGCCAGCGAGGGCGAC GGCGGCGCGGCGGCGGGAGCGGCGGCCGCGGGCGCCAGGCCGGTGTCGGTGGCTGGGAGCCCCCTGAGC CCCGGGCCGGTGCGCGCGCCCCTGAGCCGCTCGCACACCCTGAGCGGCGGCTGCGGCGGCCGCGGGAGC AAGGTGGCGCTGCTGCCGGCAGGGGGCGCGCTGCAACACAGCCGCTCCATGTCCATGCCCGTGGCGCAC TCGCCGCCCGCCGCCACCAGCCCCGGCTCCCTGTCGTCCAGCAGCGGCCACGGCTCGGGCTCCTACCCG CCGCCGCCCGGCCCGCACCCGCCTCTGCCGCATCCGCTGCACCACGGCCCCGGCCAGCGGCCCTCCAGC GGCAGCGCCTCCGCCTCGGGCTCCCCCAGCGACCCCGGCTTCATGTCCCTGGACGAGTACGGCTCCAGC CCAGGCGACCTGCGCGCCTTCTGCAGCCACCGAAGCAACACGCCCGAGTCCATCGCGGAGACGCCCCCG GCCCGAGACGGCGGCGGCGGCGGTGAGTTCTACGGGTACATGACCATGGACAGGCCCCTGAGCCACTGT GGCCGCTCCTACCGCCGGGTCTCGGGGGACGCGGCCCAGGACCTGGACCGAGGGCTGCGCAAGAGGACC TACTCCCTGACCACGCCAGCCCGGCAGCGGCCGGTGCCCCAGCCCTCCTCTGCCTCGCTGGATGAATAC ACCCTGATGCGGGCCACCTTCTCGGGCAGCGCGGGCCGCCTCTGCCCGTCCTGCCCCGCGTCCTCTCCC AAGGTGGCCTACCACCCCTACCCAGAGGACTACGGAGACATCGAGATCGGCTCCCACAGGAGCTCCAGC AGCAACCTGGGGGCAGACGACGGCTACATGCCCATGACGCCCGGCGCGGCCCTCGCGGGCAGTGGGAGC GGCAGCTGCAGGAGCGACGACTACATGCCCATGAGCCCCGCCAGCGTGTCCGCCCCCAAGCAGATCTTG CAGCCCAGGGCCGCCGCCGCCGCCGCCGCCGCCGTGCCTTCTGCGGGGCCTGCGGGGCCAGCACCCACC TCTGCGGCGGGCAGGACATTCCCGGCGAGCGGGGGCGGCTACAAGGCCAGCTCGCCCGCCGAGAGCTCC CCCGAGGACAGTGGGTACATGCGCATGTGGTGCGGTTCCAAGCTGTCCATGGAGCATGCAGATGGCAAG CTGCTGCCCAACGGGGACTACCTCAACGTGTCCCCCAGCGACGCGGTCACCACGGGCACCCCGCCCGAC TTCTTCTCCGCAGCCCTGCACCCCGGCGGGGAGCCGCTCAGGGGCGTTCCCGGCTGCTGCTACAGCTCC TTGCCCCGCTCCTACAAGGCCCCCTACACCTGTGGCGGGGACAGCGACCAGTACGTGCTCATGAGCTCC CCTGTGGGGCGCATCCTGGAGGAGGAGCGTCTGGAGCCTCAGGCCACGCCAGGGCCCAGCCAGGCGGCC AGCGCCTTCGGGGCCGGCCCCACGCAGCCCCCTCACCCTGTAGTGCCTTCGCCCGTGCGGCCTAGCGGC GGCAGCCCGGAGGGCTTCTTGGGCCAGCGCGGCCGGGCGGTGAGGCCCACGCGCCTGTCCCTGGAGGGG CTGCCCAGCCTGCCCAGCATGCACGAGTACCCACTGCCACCGGAGCCCAAGAGCCCCGGCGAGTACATC AACATCGACTTTGGCGAGCCCGGGGCCCGCCTGTCGCCGCCCGCGCCTCCCCTGCTGGCGTCGGCGGCC TCGTCCTCCTCGCTCTTGTCCGCCAGCAGCCCGGCCTCGTCGCTGGGCTCAGGCACCCCGGGCACCAGC AGCGACAGCCGGCAGCGGTCTCCGCTCTCCGACTACATGAACCTCGACTTCAGCTCCCCCAAGTCTCCT AAGCCGGGCGCCCCGAGCGGCCACCCCGTGGGCTCCTTGGACGGCCTCCTGTCCCCCGAGGCCTCCTCC CCGTATCCGCCGTTGCCCCCGCGTCCGTCCGCGTCCCCGTCGTCGTCTCTGCAGCCGCCGCCACCGCCG CCGGCCCCGGGGGAGCTGTACCGCCTGCCCCCCGCCTCGGCCGTTGCCACCGCCCAGGGCCCGGGCGCC GCCTCATCGTTGTCCTCGGACACCGGGGACAATGGTGACTACACCGAGATGGCTTTTGGTGTGGCCGCC ACCCCGCCGCAACCTATCGCGGCCCCCCCGAAGCCAGAAGCTGCCCGCGTGGCCAGCCCGACGTCGGGC GTGAAGAGGCTGAGCCTCATGGAGCAGGTGTCGGGAGTCGAGGCCTTCCTGCAGGCCAGCCAGCCCCCG GACCCCCACCGCGGCGCCAAGGTCATCCGCGCAGACCCGCAGGGGGGCCGCCGCCGCCACAGTTCCGAG ACCTTCTCCTCCACCACGACGGTCACCCCCGTGTCCCCGTCCTTCGCCCACAACCCCAAGCGCCACAAC TCGGCCTCCGTGGAAAATGTCTCTCTCAGGAAAAGCAGCGAGGGCGGCGTGGGTGTCGGCCCTGGAGGG GGCGACGAGCCGCCCACCTCCCCACGACAGTTGCAGCCGGCGCCCCCTTTGGCACCGCAGGGCCGGCCG TGGACCCCGGGTCAGCCCGGGGGCTTGGTCGGTTGTCCTGGGAGCGGTGGATCGCCCATGCGCAGAGAG ACCTCTGCCGGCTTCCAGAATGGTCTCAACTACATCGCCATCGACGTGAGGGAGGAGCCCGGGCTGCCA CCCCAGCCGCAGCCGCCGCCGCCGCCGCTTCCTCAGCCGGGAGACAAGAGCTCCTGGGGCCGGACCCGA AGCCTCGGGGGTCTCATCAGCGCTGTGGGCGTCGGCAGCACCGGCGGCGGGTGCGGGGGGCCGGGTCCC GGTGCCCTGCCCCCTGCCAACACCTACGCCAGCATTGACTTCTTGTCCCACCACTTGAAGGAGGCCACC ATCGTGAAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC212413
Blue=ORF Red=Cloning site Green=Tag(s) MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEATAGGGSAPQ PPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRA LTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGL GQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADD SVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTD SLAATPPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGS KVALLPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSS GSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHC GRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSP KVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPASVSAPKQIL QPRAAAAAAAAVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGK LLPNGDYLNVSPSDAVTTGTPPDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSS PVGRILEEERLEPQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGSPEGFLGQRGRAVRPTRLSLEG LPSLPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTS SDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQPPPPP PAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIAAPPKPEAARVASPTSG VKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHN SASVENVSLRKSSEGGVGVGPGGGDEPPTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRE TSAGFQNGLNYIAIDVREEPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGP GALPPANTYASIDFLSHHLKEATIVKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003749 |
| ORF Size | 4014 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 7014 bp |
| RefSeq ORF | 4017 bp |
| Locus ID | 8660 |
| UniProt ID | Q9Y4H2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus |
| MW | 137.3 kDa |
| Gene Summary | This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Recurrent Amplification at 13q34 Targets at CUL4A, IRS2, and TFDP1 As an Independent Adverse Prognosticator in Intrahepatic Cholangiocarcinoma
,Liu, TT;You, HL;Weng, SW;Wei, YC;Eng, HL;Huang, WT;,
PLoS ONE
,PubMed ID 26684807
[IRS2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212413L1 | Lenti ORF clone of Human insulin receptor substrate 2 (IRS2), Myc-DDK-tagged |
CNY 16,128.00 |
|
| RC212413L2 | Lenti ORF clone of Human insulin receptor substrate 2 (IRS2), mGFP tagged |
CNY 15,200.00 |
|
| RC212413L3 | Lenti ORF clone of Human insulin receptor substrate 2 (IRS2), Myc-DDK-tagged |
CNY 15,200.00 |
|
| RC212413L4 | Lenti ORF clone of Human insulin receptor substrate 2 (IRS2), mGFP tagged |
CNY 16,128.00 |
|
| RG212413 | IRS2 (tGFP-tagged) - Human insulin receptor substrate 2 (IRS2) |
CNY 15,328.00 |
|
| SC303374 | IRS2 (untagged)-Human insulin receptor substrate 2 (IRS2) |
CNY 13,744.00 |
