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ADCY8 (NM_001115) Human Tagged ORF Clone
CAT＃: RC211815
ADCY8 (Myc-DDK-tagged)-Human adenylate cyclase 8 (brain) (ADCY8)
ORF Plasmid: tGFP
"NM_001115" in other vectors (4)
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CNY 8,712.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AC8; ADCY3; HBAC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211815 representing NM_001115
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCTCCGATGTGCGCTGCCTTACAGGCAGCGAGGAACTCTACACCATCCACCCGACGCCCCCGG CCGGCGACGGCAGGAGCGCCTCCCGGCCGCAGCGGCTGCTGTGGCAGACGGCGGTGCGACACATCACGGA GCAGCGCTTCATTCACGGGCACCGGGGAGGCAGCGGCAGCGGGAGTGGAGGCTCGGGCAAAGCCTCGGAC CCTGCGGGCGGCGGCCCCAACCACCACACGCCGCAGCTGTCAGGCGACTCGGCGCTGCCCCTCTACTCGC TGGGCCCGGGAGAGCGAGCGCACAGCACCTGCGGCACCAAAGTCTTCCCGGAACGCAGCGGGAGCGGCAG TGCCAGCGGCAGCGGAGGCGGGGGCGACCTGGGCTTCCTGCACCTTGACTGTGCCCCTAGCAACTCGGAT TTCTTTCTTAATGGGGGCTATAGCTACCGAGGGGTCATTTTCCCCACCCTGCGCAACTCCTTCAAATCTC GGGATTTGGAACGCCTCTACCAGCGCTATTTCTTGGGCCAAAGGCGCAAATCGGAAGTGGTGATGAACGT GCTGGACGTGCTGACCAAACTCACTCTCTTGGTCCTACACTTGAGCCTGGCCTCGGCCCCCATGGACCCG CTCAAGGGCATCCTGCTGGGCTTCTTCACCGGCATTGAGGTAGTGATCTGCGCCCTGGTGGTGGTCAGGA AGGACACCACCTCCCACACGTACCTGCAGTACAGCGGCGTGGTCACCTGGGTGGCCATGACCACCCAGAT CCTGGCAGCAGGCCTCGGCTACGGGCTCCTGGGCGACGGCATAGGCTACGTGCTCTTCACGCTCTTCGCC ACCTACAGTATGCTGCCGCTGCCGCTCACCTGGGCCATCCTGGCCGGCCTGGGCACCTCGCTGCTGCAGG TCATCCTCCAAGTGGTCATACCCCGGCTGGCGGTCATTTCCATCAACCAGGTTGTGGCCCAGGCAGTGCT ATTCATGTGTATGAACACAGCTGGAATCTTCATCAGTTACCTGTCAGACCGTGCCCAGCGCCAAGCTTTC CTGGAGACTCGGAGGTGTGTGGAGGCCAGGCTGCGCCTGGAGACAGAGAACCAAAGACAGGAGCGGCTCG TGCTTTCTGTGCTCCCCCGGTTTGTTGTCCTGGAAATGATCAACGACATGACCAATGTGGAAGATGAGCA CCTGCAGCACCAGTTCCATCGGATCTACATCCATCGCTATGAGAACGTCAGTATTCTTTTTGCAGATGTT AAAGGATTTACCAACCTCTCCACGACCTTGTCTGCTCAGGAGCTGGTCAGGATGCTCAACGAGCTCTTTG CCAGATTTGATCGACTGGCCCATGAGCATCACTGCCTTCGTATTAAAATCCTGGGGGACTGCTACTACTG CGTGTCTGGACTTCCTGAGCCCCGCCAGGACCATGCCCACTGCTGTGTTGAAATGGGTCTCAGCATGATC AAAACCATCAGGTATGTGCGGTCAAGGACAAAACACGATGTTGACATGAGGATTGGAATCCACTCCGGCT CGGTGCTGTGCGGTGTTTTGGGACTAAGGAAGTGGCAGTTTGATGTCTGGTCTTGGGATGTGGATATTGC AAACAAACTCGAATCTGGAGGAATCCCTGGGAGGATTCACATTTCCAAAGCCACGCTGGACTGTCTCAAC GGTGACTATAACGTGGAAGAGGGCCATGGTAAAGAGAGGAATGAATTCCTGAGGAAGCATAATATCGAAA CTTACTTAATTAAGCAGCCTGAGGACAGTCTGCTGTCCTTGCCTGAAGATATCGTCAAGGAGTCAGTGAG CTCCTCAGACCGGAGAAACAGTGGGGCCACATTCACTGAAGGATCCTGGAGCCCTGAACTGCCCTTTGAT AATATCGTGGGGAAACAGAATACTCTGGCTGCCCTAACAAGAAATTCAATAAATCTGCTTCCAAACCATC TTGCACAAGCTTTGCATGTCCAGTCTGGGCCTGAGGAAATTAACAAGAGAATAGAACATACCATCGACTT GCGGAGTGGCGATAAATTGAGAAGAGAGCATATCAAGCCATTCTCACTGATGTTTAAAGACTCCAGCCTG GAGCACAAGTATTCTCAAATGAGGGATGAAGTGTTCAAGTCAAACTTGGTCTGTGCATTTATCGTTCTTC TATTTATCACGGCAATACAAAGTTTGCTTCCTTCTTCAAGAGTGATGCCAATGACCATCCAGTTCTCCAT TCTGATTATGCTGCACTCGGCTCTGGTCCTCATCACCACAGCAGAGGATTATAAATGTTTGCCCCTCATC CTCCGGAAAACTTGCTGTTGGATTAATGAGACCTATTTGGCCCGGAACGTCATCATCTTTGCATCCATTT TGATTAATTTCCTGGGTGCCATCTTAAATATCCTGTGGTGTGATTTTGACAAGTCGATACCCTTGAAGAA CCTGACTTTCAATTCCTCAGCTGTGTTTACAGATATCTGCTCCTACCCAGAGTACTTTGTCTTCACGGGG GTGTTGGCCATGGTGACCTGTGCAGTTTTCCTCCGGCTGAACTCCGTCCTGAAGCTGGCAGTGCTGCTGA TCATGATTGCCATCTATGCCCTGCTCACTGAGACCGTCTACGTAGGCCTCTTTCTGCGTTATGACAACCT CAACCACAGTGGAGAAGATTTCCTGGGGACCAAGGAGGTATCACTGCTACTGATGGCCATGTTCCTCCTG GCTGTGTTCTACCATGGACAGCAGCTGGAGTACACAGCCCGCCTGGACTTCCTTTGGCGAGTACAGGCCA AAGAGGAGATCAATGAGATGAAGGAGCTGAGGGAACACAATGAGAACATGCTCCGGAATATCTTACCCAG CCATGTGGCCCGCCATTTCCTAGAGAAGGACCGAGACAATGAGGAGCTGTATTCTCAATCCTATGATGCT GTTGGGGTGATGTTTGCCTCCATCCCAGGATTTGCGGACTTTTACTCTCAGACTGAAATGAATAACCAGG GAGTGGAATGCCTGCGCTTGCTCAATGAGATCATTGCTGACTTCGATGAGTTGCTTGGTGAAGACCGATT TCAAGACATTGAAAAGATTAAGACCATTGGCAGCACCTACATGGCCGTGTCAGGCCTGTCACCTGAAAAA CAGCAATGTGAAGACAAGTGGGGACATTTGTGTGCTCTGGCTGACTTCTCACTCGCCCTGACAGAAAGCA TACAGGAGATCAACAAGCATTCATTCAACAATTTTGAACTCCGGATTGGCATCAGCCACGGCTCAGTGGT AGCTGGCGTTATCGGCGCTAAGAAACCACAGTATGACATTTGGGGCAAAACTGTGAACCTGGCAAGCCGA ATGGACAGCACGGGGGTTAGTGGCCGGATCCAAGTCCCAGAGGAGACCTATCTCATCCTGAAGGACCAGG GCTTTGCCTTTGATTACCGAGGGGAGATCTATGTGAAGGGTATCAGTGAACAGGAAGGAAAAATCAAAAC GTACTTTCTTCTGGGAAGAGTCCAACCCAACCCATTCATCTTGCCCCCAAGAAGACTGCCTGGGCAGTAC TCCCTGGCCGCGGTTGTCCTGGGACTTGTCCAGTCCCTCAATAGGCAAAGGCAGAAGCAGCTACTCAATG AGAACAACAACACAGGAATCATCAAGGGTCATTACAACCGGCGGACTTTGTTGTCACCCAGCGGCACAGA GCCTGGAGCCCAGGCTGAAGGCACCGACAAATCTGATTTGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211815 representing NM_001115
Red=Cloning site Green=Tags(s) MELSDVRCLTGSEELYTIHPTPPAGDGRSASRPQRLLWQTAVRHITEQRFIHGHRGGSGSGSGGSGKASD PAGGGPNHHTPQLSGDSALPLYSLGPGERAHSTCGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSD FFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDP LKGILLGFFTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFA TYSMLPLPLTWAILAGLGTSLLQVILQVVIPRLAVISINQVVAQAVLFMCMNTAGIFISYLSDRAQRQAF LETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADV KGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMI KTIRYVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLN GDYNVEEGHGKERNEFLRKHNIETYLIKQPEDSLLSLPEDIVKESVSSSDRRNSGATFTEGSWSPELPFD NIVGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSL EHKYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRVMPMTIQFSILIMLHSALVLITTAEDYKCLPLI LRKTCCWINETYLARNVIIFASILINFLGAILNILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTG VLAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETVYVGLFLRYDNLNHSGEDFLGTKEVSLLLMAMFLL AVFYHGQQLEYTARLDFLWRVQAKEEINEMKELREHNENMLRNILPSHVARHFLEKDRDNEELYSQSYDA VGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEK QQCEDKWGHLCALADFSLALTESIQEINKHSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASR MDSTGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQY SLAAVVLGLVQSLNRQRQKQLLNENNNTGIIKGHYNRRTLLSPSGTEPGAQAEGTDKSDLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001115 |
| ORF Size | 3753 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001115.3 |
| RefSeq Size | 6005 bp |
| RefSeq ORF | 3756 bp |
| Locus ID | 114 |
| UniProt ID | P40145 |
| Domains | CYCc |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction, Vascular smooth muscle contraction |
| MW | 139.9 kDa |
| Gene Summary | Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. The enzymatic activity is under the control of several hormones, and different polypeptides participate in the transduction of the signal from the receptor to the catalytic moiety. Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211815L3 | Lenti ORF clone of Human adenylate cyclase 8 (brain) (ADCY8), Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC211815L4 | Lenti ORF clone of Human adenylate cyclase 8 (brain) (ADCY8), mGFP tagged |
CNY 11,500.00 |
|
| RG211815 | ADCY8 (tGFP-tagged) - Human adenylate cyclase 8 (brain) (ADCY8) |
CNY 10,550.00 |
|
| SC119443 | ADCY8 (untagged)-Human adenylate cyclase 8 (brain) (ADCY8) |
CNY 8,720.00 |
