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HGFAC (NM_001528) Human Tagged ORF Clone
CAT＃: RC211348
- TrueORF®
HGFAC (Myc-DDK-tagged)-Human HGF activator (HGFAC)
ORF Plasmid: tGFP
"NM_001528" in other vectors (4)
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CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HGFA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211348 representing NM_001528
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGCTGGGCCTGGGTCCCCAGCCCCTGGCCCCCACCGGGGCTGGGCCCCTTCCTCCTCCTCCTCC TGCTGCTGCTGCTGCTGCCACGGGGGTTCCAGCCCCAGCCTGGCGGGAACCGTACGGAGTCCCCAGAACC TAATGCCACAGCGACCCCTGCGATCCCCACTATCCTGGTGACCTCTGTGACCTCTGAGACCCCAGCAACA AGTGCTCCAGAGGCAGAGGGACCCCAAAGTGGGGGGCTCCCGCCCCCGCCCAGGGCAGTTCCCTCGAGCA GTAGCCCCCAGGCCCAAGCACTCACCGAGGACGGGAGGCCCTGCAGGTTCCCCTTCCGCTACGGGGGCCG CATGCTGCATGCCTGCACTTCGGAGGGCAGTGCACACAGGAAGTGGTGTGCCACAACTCACAACTACGAC CGGGACAGGGCCTGGGGCTACTGTGTGGAGGCCACCCCGCCTCCAGGGGGCCCAGCTGCCCTGGATCCCT GTGCCTCCGGCCCCTGCCTCAATGGAGGCTCCTGCTCCAATACCCAGGACCCCCAGTCCTATCACTGCAG CTGCCCCCGGGCCTTCACCGGCAAGGACTGCGGCACAGAGAAATGCTTTGATGAGACCCGCTACGAGTAC CTGGAGGGGGGCGACCGCTGGGCCCGCGTGCGCCAGGGCCACGTGGAACAGTGCGAGTGCTTCGGGGGCC GGACCTGGTGCGAAGGCACCCGACATACAGCTTGTCTGAGCAGCCCTTGCCTGAACGGGGGCACCTGCCA CCTGATCGTGGCCACCGGGACCACCGTGTGTGCCTGCCCACCAGGCTTCGCTGGACGGCTCTGCAACATC GAGCCTGATGAGCGCTGCTTCTTGGGGAACGGCACTGGGTACCGTGGCGTGGCCAGCACCTCAGCCTCGG GCCTCAGCTGCCTGGCCTGGAACTCCGATCTGCTCTACCAGGAGCTGCACGTGGACTCCGTGGGCGCCGC GGCCCTGCTGGGCCTGGGCCCCCATGCCTACTGCCGGAATCCGGACAATGACGAGAGGCCCTGGTGCTAC GTGGTGAAGGACAGCGCGCTCTCCTGGGAGTACTGCCGCCTGGAGGCCTGCGAATCCCTCACCAGAGTCC AACTGTCACCGGATCTCCTGGCGACCCTGCCTGAGCCAGCCTCCCCGGGGCGCCAGGCCTGCGGCAGGAG GCACAAGAAGAGGACGTTCCTGCGGCCACGTATCATCGGCGGCTCCTCCTCGCTGCCCGGCTCGCACCCC TGGCTGGCCGCCATCTACATCGGGGACAGCTTCTGCGCCGGGAGCCTGGTCCACACCTGCTGGGTGGTGT CGGCCGCCCACTGCTTCTCCCACAGCCCCCCCAGGGACAGCGTCTCCGTGGTGCTGGGCCAGCACTTCTT CAACCGCACGACGGACGTGACGCAGACCTTCGGCATCGAGAAGTACATCCCGTACACCCTGTACTCGGTG TTCAACCCCAGCGACCACGACCTCGTCCTGATCCGGCTGAAGAAGAAAGGGGACCGCTGTGCCACACGCT CGCAGTTCGTGCAGCCCATCTGCCTGCCCGAGCCCGGCAGCACCTTCCCCGCAGGACACAAGTGCCAGAT TGCGGGCTGGGGCCACTTGGATGAGAACGTGAGCGGCTACTCCAGCTCCCTGCGGGAGGCCCTGGTCCCC CTGGTCGCCGACCACAAGTGCAGCAGCCCTGAGGTCTACGGCGCCGACATCAGCCCCAACATGCTCTGTG CCGGCTACTTCGACTGCAAGTCCGACGCCTGCCAGGGGGACTCAGGGGGGCCCCTGGCCTGCGAGAAGAA CGGCGTGGCTTACCTCTACGGCATCATCAGCTGGGGTGACGGCTGCGGGCGGCTCCACAAGCCGGGGGTC TACACCCGCGTGGCCAACTATGTGGACTGGATCAACGACCGGATACGGCCTCCCAGGCGGCTTGTGGCTC CCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211348 representing NM_001528
Red=Cloning site Green=Tags(s) MGRWAWVPSPWPPPGLGPFLLLLLLLLLLPRGFQPQPGGNRTESPEPNATATPAIPTILVTSVTSETPAT SAPEAEGPQSGGLPPPPRAVPSSSSPQAQALTEDGRPCRFPFRYGGRMLHACTSEGSAHRKWCATTHNYD RDRAWGYCVEATPPPGGPAALDPCASGPCLNGGSCSNTQDPQSYHCSCPRAFTGKDCGTEKCFDETRYEY LEGGDRWARVRQGHVEQCECFGGRTWCEGTRHTACLSSPCLNGGTCHLIVATGTTVCACPPGFAGRLCNI EPDERCFLGNGTGYRGVASTSASGLSCLAWNSDLLYQELHVDSVGAAALLGLGPHAYCRNPDNDERPWCY VVKDSALSWEYCRLEACESLTRVQLSPDLLATLPEPASPGRQACGRRHKKRTFLRPRIIGGSSSLPGSHP WLAAIYIGDSFCAGSLVHTCWVVSAAHCFSHSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSV FNPSDHDLVLIRLKKKGDRCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVP LVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGRLHKPGV YTRVANYVDWINDRIRPPRRLVAPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001528 |
| ORF Size | 1965 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001528.4 |
| RefSeq Size | 2038 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 3083 |
| UniProt ID | Q04756 |
| Domains | KR, FN1, FN2, Tryp_SPc, EGF, EGF |
| Protein Families | Druggable Genome, Protease, Secreted Protein, Transmembrane |
| MW | 70.68 kDa |
| Gene Summary | This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211348L3 | Lenti-ORF clone of HGFAC (Myc-DDK-tagged)-Human HGF activator (HGFAC) |
CNY 6,460.00 |
|
| RC211348L4 | Lenti-ORF clone of HGFAC (mGFP-tagged)-Human HGF activator (HGFAC) |
CNY 6,460.00 |
|
| RG211348 | HGFAC (tGFP-tagged) - Human HGF activator (HGFAC) |
CNY 5,040.00 |
|
| SC119188 | HGFAC (untagged)-Human HGF activator (HGFAC) |
CNY 10,450.00 |
