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5HT5A receptor (HTR5A) (NM_024012) Human Tagged ORF Clone
CAT＃: RC211300
HTR5A (Myc-DDK-tagged)-Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A)
ORF Plasmid: tGFP
"NM_024012" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5-HT5A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211300 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTACCTGTGAACCTAACCTCCTTTTCCCTCTCCACCCCCTCCCCTTTGGAGACCAACCACAGCC TCGGCAAAGACGACCTGCGCCCCAGCTCGCCCCTGCTCTCGGTCTTCGGAGTGCTTATTCTCACCTTGCT GGGCTTTCTGGTGGCGGCGACGTTCGCCTGGAACCTGCTGGTGCTGGCGACCATCCTCCGTGTACGCACC TTCCACCGCGTGCCCCACAACCTGGTGGCATCCATGGCCGTCTCGGATGTCCTGGTGGCCGCGCTGGTCA TGCCGCTGAGCCTGGTGCACGAGCTGTCCGGGCGCCGCTGGCAGCTAGGTCGGAGGCTGTGCCAGCTTTG GATCGCGTGCGACGTGCTTTGCTGCACGGCCAGCATCTGGAACGTGACGGCCATAGCCCTGGACCGCTAC TGGTCCATCACGCGCCACATGGAATACACGCTCCGCACCCGCAAGTGCGTCTCCAACGTCATGATCGCGC TCACCTGGGCACTCTCCGCTGTCATCTCTCTGGCCCCGCTGCTTTTTGGCTGGGGAGAGACGTACTCTGA GGGCAGCGAGGAGTGCCAGGTAAGCCGCGAGCCTTCCTACGCCGTGTTCTCCACCGTAGGCGCCTTCTAC CTGCCGCTCTGTGTGGTGCTCTTCGTGTACTGGAAGATCTACAAGGCTGCCAAGTTCCGCGTGGGCTCCA GGAAGACCAATAGCGTCTCACCCATATCCGAAGCTGTGGAGGTGAAGGACTCTGCCAAACAGCCCCAGAT GGTGTTCACGGTCCGCCACGCCACCGTCACCTTCCAGCCAGAAGGGGACACGTGGCGGGAGCAGAAGGAG CAGCGGGCCGCCCTCATGGTGGGCATCCTCATTGGCGTGTTCGTGCTCTGCTGGATCCCCTTCTTTCTCA CCGAGCTCATCAGTCCCCTCTGCTCCTGTGACATCCCCGCCATCTGGAAAAGCATCTTCCTGTGGCTTGG CTACTCCAACTCCTTCTTTAACCCCCTGATCTATACGGCTTTCAACAAGAACTACAACAGCGCCTTCAAG AACTTCTTTTCTAGGCAACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211300 protein sequence
Red=Cloning site Green=Tags(s) MDLPVNLTSFSLSTPSPLETNHSLGKDDLRPSSPLLSVFGVLILTLLGFLVAATFAWNLLVLATILRVRT FHRVPHNLVASMAVSDVLVAALVMPLSLVHELSGRRWQLGRRLCQLWIACDVLCCTASIWNVTAIALDRY WSITRHMEYTLRTRKCVSNVMIALTWALSAVISLAPLLFGWGETYSEGSEECQVSREPSYAVFSTVGAFY LPLCVVLFVYWKIYKAAKFRVGSRKTNSVSPISEAVEVKDSAKQPQMVFTVRHATVTFQPEGDTWREQKE QRAALMVGILIGVFVLCWIPFFLTELISPLCSCDIPAIWKSIFLWLGYSNSFFNPLIYTAFNKNYNSAFK NFFSRQH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024012 |
| ORF Size | 1071 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024012.4 |
| RefSeq Size | 4572 bp |
| RefSeq ORF | 1074 bp |
| Locus ID | 3361 |
| UniProt ID | P47898 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Neuroactive ligand-receptor interaction |
| MW | 40.3 kDa |
| Gene Summary | The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been implicated in a wide range of psychiatric conditions and also has vasoconstrictive and vasodilatory effects. The gene described in this record is a member of 5-hydroxytryptamine (serotonin) receptor family and encodes a multi-pass membrane protein that functions as a receptor for 5-hydroxytryptamine and couples to G-proteins. This protein has been shown to function in part through the regulation of intracellular Ca2+ mobilization. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211300L1 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211300L2 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), mGFP tagged |
CNY 5,890.00 |
|
| RC211300L3 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211300L4 | Lenti ORF clone of Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), mGFP tagged |
CNY 5,890.00 |
|
| RG211300 | HTR5A (tGFP-tagged) - Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A) |
CNY 4,370.00 |
|
| SC112321 | HTR5A (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A) |
CNY 3,656.00 |
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