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DGKB (NM_145695) Human Tagged Lenti ORF Clone
CAT＃: RC211233L3
- LentiORF®
Lenti ORF clone of Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2, Myc-DDK-tagged
"NM_145695" in other vectors (4)
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CNY 5,890.00
Product images
CNY 1,999.00
CNY 2,700.00
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DAGK2; DGK; DGK-BETA |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC211233).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145695 |
| ORF Size | 2319 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145695.1, NP_663733.1 |
| RefSeq Size | 3360 bp |
| RefSeq ORF | 2322 bp |
| Locus ID | 1607 |
| UniProt ID | Q9Y6T7 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| MW | 87.1 kDa |
| Gene Summary | Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211233 | DGKB (Myc-DDK-tagged)-Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2 |
CNY 3,990.00 |
|
| RC211233L4 | Lenti ORF clone of Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG211233 | DGKB (tGFP-tagged) - Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2 |
CNY 4,370.00 |
|
| SC306260 | DGKB (untagged)-Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2 |
CNY 7,220.00 |
