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Nkx2.2 (NKX2-2) (NM_002509) Human Tagged ORF Clone
CAT＃: RC210419
NKX2 (Myc-DDK-tagged)-Human NK2 homeobox 2 (NKX2-2)
ORF Plasmid: tGFP
"NM_002509" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NKX2.2; NKX2B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210419 representing NM_002509
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTGACCAACACAAAGACGGGGTTTTCGGTCAAGGACATCTTAGACCTGCCGGACACCAACGATG AGGAGGGCTCTGTGGCCGAAGGTCCGGAGGAAGAGAACGAGGGGCCCGAGCCAGCCAAGAGGGCCGGGCC GCTGGGGCAGGGCGCCCTGGACGCGGTGCAGAGCCTGCCCCTGAAGAACCCCTTCTACGACAGCAGCGAC AACCCGTACACGCGCTGGCTGGCCAGCACCGAGGGCCTTCAGTACTCCCTGCACGGTCTGGCTGCCGGGG CGCCCCCTCAGGACTCAAGCTCCAAGTCCCCGGAGCCCTCGGCCGACGAGTCACCGGACAATGACAAGGA GACCCCGGGCGGCGGGGGGGACGCCGGCAAGAAGCGAAAGCGGCGAGTGCTTTTCTCCAAGGCGCAGACC TACGAGCTGGAGCGGCGCTTTCGGCAGCAGCGGTACCTGTCGGCGCCCGAGCGCGAACACCTGGCCAGCC TCATCCGCCTCACGCCCACGCAGGTCAAGATCTGGTTCCAGAACCACCGCTACAAGATGAAGCGCGCCCG GGCCGAGAAAGGTATGGAGGTGACGCCCCTGCCCTCGCCGCGCCGGGTGGCCGTGCCCGTCTTGGTCAGG GACGGCAAACCATGTCACGCGCTCAAAGCCCAGGACCTGGCAGCCGCCACCTTCCAGGCGGGCATTCCCT TTTCTGCCTACAGCGCGCAGTCGCTGCAGCACATGCAGTACAACGCCCAGTACAGCTCGGCCAGCACCCC CCAGTACCCGACAGCACACCCCCTGGTCCAGGCCCAGCAGTGGACTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210419 representing NM_002509
Red=Cloning site Green=Tags(s) MSLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQGALDAVQSLPLKNPFYDSSD NPYTRWLASTEGLQYSLHGLAAGAPPQDSSSKSPEPSADESPDNDKETPGGGGDAGKKRKRRVLFSKAQT YELERRFRQQRYLSAPEREHLASLIRLTPTQVKIWFQNHRYKMKRARAEKGMEVTPLPSPRRVAVPVLVR DGKPCHALKAQDLAAATFQAGIPFSAYSAQSLQHMQYNAQYSSASTPQYPTAHPLVQAQQWTW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002509 |
| ORF Size | 819 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002509.4 |
| RefSeq Size | 2092 bp |
| RefSeq ORF | 822 bp |
| Locus ID | 4821 |
| UniProt ID | O95096 |
| Protein Families | Transcription Factors |
| Protein Pathways | Maturity onset diabetes of the young |
| MW | 30 kDa |
| Gene Summary | The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
NKL homeobox gene NKX2-2 is aberrantly expressed in Hodgkin lymphoma
,Nagel, S;MacLeod, RAF;Pommerenke, C;Meyer, C;Kaufmann, M;Drexler, HG;,
Oncotarget
,PubMed ID 30680064
[Nkx2.2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210419L1 | Lenti ORF clone of Human NK2 homeobox 2 (NKX2-2), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210419L2 | Lenti ORF clone of Human NK2 homeobox 2 (NKX2-2), mGFP tagged |
CNY 5,890.00 |
|
| RC210419L3 | Lenti ORF clone of Human NK2 homeobox 2 (NKX2-2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210419L4 | Lenti ORF clone of Human NK2 homeobox 2 (NKX2-2), mGFP tagged |
CNY 4,800.00 |
|
| RG210419 | NKX2 (tGFP-tagged) - Human NK2 homeobox 2 (NKX2-2) |
CNY 4,000.00 |
|
| SC303209 | NKX2 (untagged)-Human NK2 homeobox 2 (NKX2-2) |
CNY 2,400.00 |
