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G2A (GPR132) (NM_013345) Human Tagged ORF Clone
CAT＃: RC210284
- TrueORF®
GPR132 (Myc-DDK-tagged)-Human G protein-coupled receptor 132 (GPR132)
ORF Plasmid: tGFP
"NM_013345" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | G2A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210284 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCCAATGCTACTGAAAAACGGTTACAATGGAAACGCCACCCCAGTGACCACCACTGCCCCGTGGG CCTCCCTGGGCCTCTCCGCCAAGACCTGCAACAACGTGTCCTTCGAAGAGAGCAGGATAGTCCTGGTCGT GGTGTACAGCGCGGTGTGCACGCTGGGGGTGCCGGCCAACTGCCTGACTGCGTGGCTGGCGCTGCTGCAG GTACTGCAGGGCAACGTGCTGGCCGTCTACCTGCTCTGCCTGGCACTCTGCGAGCTGCTGTACACAGGCA CGCTGCCACTCTGGGTCATCTATATCCGCAACCAGCACCGCTGGACCCTAGGCCTGCTGGCCTGCAAGGT GACCGCCTACATCTTCTTCTGCAACATCTACGTCAGCATCCTCTTCCTGTGCTGCATCTCCTGCGACCGC TTCGTGGCCGTGGTGTACGCGCTGGAGAGTCGGGGCCGCCGCCGCCGGAGGACCGCCATCCTCATCTCCG CCTGCATCTTCATCCTCGTCGGGATCGTTCACTACCCGGTGTTCCAGACGGAAGACAAGGAGACCTGCTT TGACATGCTGCAGATGGACAGCAGGATTGCCGGGTACTACTACGCCAGGTTCACCGTTGGCTTTGCCATC CCTCTCTCCATCATCGCCTTCACCAACCACCGGATTTTCAGGAGCATCAAGCAGAGCATGGGCTTAAGCG CTGCCCAGAAGGCCAAGGTGAAGCACTCGGCCATCGCGGTGGTTGTCATCTTCCTAGTCTGCTTCGCCCC GTACCACCTGGTTCTCCTCGTCAAAGCCGCTGCCTTTTCCTACTACAGAGGAGACAGGAACGCCATGTGC GGCTTGGAGGAAAGGCTGTACACAGCCTCTGTGGTGTTTCTGTGCCTGTCCACGGTGAACGGCGTGGCTG ACCCCATTATCTACGTGCTGGCCACGGACCATTCCCGCCAAGAAGTGTCCAGAATCCATAAGGGGTGGAA AGAGTGGTCCATGAAGACAGACGTCACCAGGCTCACCCACAGCAGGGACACCGAGGAGCTGCAGTCGCCC GTGGCCCTTGCAGACCACTACACCTTCTCCAGGCCCGTGCACCCACCAGGGTCACCATGCCCTGCAAAGA GGCTGATTGAGGAGTCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210284 protein sequence
Red=Cloning site Green=Tags(s) MCPMLLKNGYNGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQ VLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDR FVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAI PLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMC GLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSP VALADHYTFSRPVHPPGSPCPAKRLIEESC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013345 |
| ORF Size | 1140 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013345.4 |
| RefSeq Size | 3788 bp |
| RefSeq ORF | 1143 bp |
| Locus ID | 29933 |
| UniProt ID | Q9UNW8 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 42.5 kDa |
| Gene Summary | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Lipid Receptor G2A-Mediated Signal Pathway Plays a Critical Role in Inflammatory Response by Promoting Classical Macrophage Activation
,Li, Q;Feng, C;Li, L;Xu, G;Gu, H;Li, S;Li, D;Liu, M;Han, S;Zheng, B;,
Journal of immunology (Baltimore, Md. : 1950) 2021
,PubMed ID 33941654
[GPR132]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210284L3 | Lenti ORF clone of Human G protein-coupled receptor 132 (GPR132), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210284L4 | Lenti ORF clone of Human G protein-coupled receptor 132 (GPR132), mGFP tagged |
CNY 5,890.00 |
|
| RG210284 | GPR132 (tGFP-tagged) - Human G protein-coupled receptor 132 (GPR132) |
CNY 4,370.00 |
|
| SC321011 | GPR132 (untagged)-Human G protein-coupled receptor 132 (GPR132) |
CNY 2,950.00 |
