United States
Germany
Japan
United Kingdom
China
MMP16 (NM_005941) Human Tagged ORF Clone
CAT＃: RC210199
MMP16 (Myc-DDK-tagged)-Human matrix metallopeptidase 16 (membrane-inserted) (MMP16)
ORF Plasmid: tGFP
"NM_005941" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 6,776.00
| Cited in 1 publication. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C8orf57; MMP-X2; MT-MMP2; MT-MMP3; MT3-MMP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210199 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTTACTCACATTCAGCACTGGAAGACGGTTGGATTTCGTGCATCATTCGGGGGTGTTTTTCTTGC AAACCTTGCTTTGGATTTTATGTGCTACAGTCTGCGGAACGGAGCAGTATTTCAATGTGGAGGTTTGGTT ACAAAAGTACGGCTACCTTCCACCGACTGACCCCAGAATGTCAGTGCTGCGCTCTGCAGAGACCATGCAG TCTGCCCTAGCTGCCATGCAGCAGTTCTATGGCATTAACATGACAGGAAAAGTGGACAGAAACACAATTG ACTGGATGAAGAAGCCCCGATGCGGTGTACCTGACCAGACAAGAGGTAGCTCCAAATTTCATATTCGTCG AAAGCGATATGCATTGACAGGACAGAAATGGCAGCACAAGCACATCACTTACAGTATAAAGAACGTAACT CCAAAAGTAGGAGACCCTGAGACTCGTAAAGCTATTCGCCGTGCCTTTGATGTGTGGCAGAATGTAACTC CTCTGACATTTGAAGAAGTTCCCTACAGTGAATTAGAAAATGGCAAACGTGATGTGGATATAACCATTAT TTTTGCATCTGGTTTCCATGGGGACAGCTCTCCCTTTGATGGAGAGGGAGGATTTTTGGCACATGCCTAC TTCCCTGGACCAGGAATTGGAGGAGATACCCATTTTGACTCAGATGAGCCATGGACACTAGGAAATCCTA ATCATGATGGAAATGACTTATTTCTTGTAGCAGTCCATGAACTGGGACATGCTCTGGGATTGGAGCATTC CAATGACCCCACTGCCATCATGGCTCCATTTTACCAGTACATGGAAACAGACAACTTCAAACTACCTAAT GATGATTTACAGGGCATCCAGAAGATATATGGTCCACCTGACAAGATTCCTCCACCTACAAGACCTCTAC CGACAGTGCCCCCACACCGCTCTATTCCTCCGGCTGACCCAAGGAAAAATGACAGGCCAAAACCTCCTCG GCCTCCAACCGGCAGACCCTCCTATCCCGGAGCCAAACCCAACATCTGTGATGGGAACTTTAACACTCTA GCTATTCTTCGTCGTGAGATGTTTGTTTTCAAGGACCAGTGGTTTTGGCGAGTGAGAAACAACAGGGTGA TGGATGGATACCCAATGCAAATTACTTACTTCTGGCGGGGCTTGCCTCCTAGTATCGATGCAGTTTATGA AAATAGCGACGGGAATTTTGTGTTCTTTAAAGGTAACAAATATTGGGTGTTCAAGGATACAACTCTTCAA CCTGGTTACCCTCATGACTTGATAACCCTTGGAAGTGGAATTCCCCCTCATGGTATTGATTCAGCCATTT GGTGGGAGGACGTCGGGAAAACCTATTTCTTCAAGGGAGACAGATATTGGAGATATAGTGAAGAAATGAA AACAATGGACCCTGGCTATCCCAAGCCAATCACAGTCTGGAAAGGGATCCCTGAATCTCCTCAGGGAGCA TTTGTACACAAAGAAAATGGCTTTACGTATTTCTACAAAGGAAAGGAGTATTGGAAATTCAACAACCAGA TACTCAAGGTAGAACCTGGATATCCAAGATCCATCCTCAAGGATTTTATGGGCTGTGATGGACCAACAGA CAGAGTTAAAGAAGGACACAGCCCACCAGATGATGTAGACATTGTCATCAAACTGGACAACACAGCCAGC ACTGTGAAAGCCATAGCTATTGTCATTCCCTGCATCTTGGCCTTATGCCTCCTTGTATTGGTTTACACTG TGTTCCAGTTCAAGAGGAAAGGAACACCCCGCCACATACTGTACTGTAAACGCTCTATGCAAGAGTGGGT G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210199 protein sequence
Red=Cloning site Green=Tags(s) MILLTFSTGRRLDFVHHSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQ SALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRRKRYALTGQKWQHKHITYSIKNVT PKVGDPETRKAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAY FPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPN DDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSIPPADPRKNDRPKPPRPPTGRPSYPGAKPNICDGNFNTL AILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQ PGYPHDLITLGSGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITVWKGIPESPQGA FVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRVKEGHSPPDDVDIVIKLDNTAS TVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005941 |
| ORF Size | 1821 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005941.5 |
| RefSeq Size | 6347 bp |
| RefSeq ORF | 1824 bp |
| Locus ID | 4325 |
| UniProt ID | P51512 |
| Protein Families | Druggable Genome, Protease, Secreted Protein, Transmembrane |
| MW | 69.5 kDa |
| Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Biochemical and spectroscopic characterization of the catalytic domain of MMP16 (cdMMP16)
,Meng, F;Yang, H;Aitha, M;George, S;Tierney, DL;Crowder, MW;,
J. Biol. Inorg. Chem.
,PubMed ID 27229514
[MMP16]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210199L1 | Lenti ORF clone of Human matrix metallopeptidase 16 (membrane-inserted) (MMP16), Myc-DDK-tagged |
CNY 9,176.00 |
|
| RC210199L2 | Lenti ORF clone of Human matrix metallopeptidase 16 (membrane-inserted) (MMP16), mGFP tagged |
CNY 5,890.00 |
|
| RC210199L3 | Lenti ORF clone of Human matrix metallopeptidase 16 (membrane-inserted) (MMP16), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210199L4 | Lenti ORF clone of Human matrix metallopeptidase 16 (membrane-inserted) (MMP16), mGFP tagged |
CNY 5,890.00 |
|
| RG210199 | MMP16 (tGFP-tagged) - Human matrix metallopeptidase 16 (membrane-inserted) (MMP16) |
CNY 4,370.00 |
|
| SC316625 | MMP16 (untagged)-Human matrix metallopeptidase 16 (membrane-inserted) (MMP16) |
CNY 2,950.00 |
