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PPM1J (NM_005167) Human Tagged ORF Clone
CAT＃: RC210062
PPM1J (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1J (PPM1J)
ORF Plasmid: tGFP
"NM_005167" in other vectors (4)
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CNY 3,760.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PP2C-zeta; PP2CZ; PP2Czeta; PPP2CZ |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210062 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAAACCGGGTGCGCTCGGCCGTGGCGCACCTGGTGAGCTCCGGGGGCGCTCCGCCTCCGCGCCCCA AATCCCCGGACCTGCCCAACGCCGCCTCGGCGCCGCCCGCCGCCGCTCCAGAAGCGCCCAGGAGCCCTCC CGCGAAGGCTGGGAGCGGGAGCGCGACGCCCGCGAAGGCTGTTGAGGCTCGAGCGAGCTTCTCCAGACCG ACCTTTCTGCAGCTGAGCCCCGGGGGGCTGCGACGCGCCGATGACCACGCGGGCCGGGCTGTGCAAAGCC CCCCGGACACGGGCCGCCGCCTGCCCTGGAGCACAGGCTACGCCGAGGTCATCAATGCTGGCAAGAGTCG GCACAATGAGGACCAGGCTTGCTGTGAAGTGGTGTATGTGGAAGGTCGGAGGAGTGTTACAGGAGTACCT AGGGAGCCTAGCCGAGGCCAGGGACTCTGCTTCTACTACTGGGGCCTATTTGATGGGCATGCAGGGGGCG GAGCTGCTGAAATGGCCTCACGGCTCCTGCATCGCCATATCCGAGAGCAGCTAAAGGACCTGGTAGAGAT ACTTCAGGACCCTTCGCCACCACCCCTCTGCCTCCCAACCACTCCGGGGACCCCAGATTCCTCCGATCCC TCTCACTTGCTTGGCCCTCAGTCCTGCTGGTCTTCACAGAAGGAAGTGAGCCACGAGAGCCTGGTAGTGG GGGCCATTGAGAATGCCTTCCAGCTCATGGATGAGCAGATGGCCCGGGAGCGGCGTGGCCACCAAGTGGA GGGGGGCTGCTGTGCACTGGTTGTGATCTACCTGCTAGGCAAGGTGTACGTGGCCAATGCAGGCGATAGC AGGGCCATCATTGTCCGGAATGGTGAAATCATTCCAATGTCCCGGGAGTTTACCCCGGAGACTGAGCGCC AGCGTCTTCAGCTGCTTGGCTTCCTGAAACCAGAGCTGCTAGGCAGTGAATTCACCCACCTTGAGTTCCC CCGCAGAGTTCTGCCCAAGGAGCTGGGGCAGAGGATGTTGTACCGGGACCAGAACATGACCGGCTGGGCC TACAAAAAGATCGAGCTGGAGGATCTCAGGTTTCCTCTGGTCTGTGGGGAGGGCAAAAAGGCTCGGGTGA TGGCCACCATTGGGGTGACCCGAGGCTTGGGAGACCACAGCCTTAAGGTCTGCAGTTCCACCCTGCCCAT CAAGCCCTTTCTCTCCTGCTTCCCTGAGGTACGAGTGTATGACCTGACACAATATGAGCACTGCCCAGAT GATGTGCTAGTCCTGGGAACAGATGGCCTGTGGGATGTCACTACTGACTGTGAGGTAGCTGCCACTGTGG ACAGGGTGCTGTCGGCCTATGAGCCTAATGACCACAGCAGGTATACAGCTCTGGCCCAAGCTCTGGTCCT GGGGGCCCGGGGTACCCCCCGAGACCGTGGCTGGCGTCTCCCCAACAACAAGCTGGGTTCCGGGGATGAC ATCTCTGTCTTCGTCATCCCCCTGGGAGGGCCAGGCAGTTACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210062 protein sequence
Red=Cloning site Green=Tags(s) MLNRVRSAVAHLVSSGGAPPPRPKSPDLPNAASAPPAAAPEAPRSPPAKAGSGSATPAKAVEARASFSRP TFLQLSPGGLRRADDHAGRAVQSPPDTGRRLPWSTGYAEVINAGKSRHNEDQACCEVVYVEGRRSVTGVP REPSRGQGLCFYYWGLFDGHAGGGAAEMASRLLHRHIREQLKDLVEILQDPSPPPLCLPTTPGTPDSSDP SHLLGPQSCWSSQKEVSHESLVVGAIENAFQLMDEQMARERRGHQVEGGCCALVVIYLLGKVYVANAGDS RAIIVRNGEIIPMSREFTPETERQRLQLLGFLKPELLGSEFTHLEFPRRVLPKELGQRMLYRDQNMTGWA YKKIELEDLRFPLVCGEGKKARVMATIGVTRGLGDHSLKVCSSTLPIKPFLSCFPEVRVYDLTQYEHCPD DVLVLGTDGLWDVTTDCEVAATVDRVLSAYEPNDHSRYTALAQALVLGARGTPRDRGWRLPNNKLGSGDD ISVFVIPLGGPGSYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005167 |
| ORF Size | 1515 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005167.5, NP_005158.5 |
| RefSeq Size | 1762 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 333926 |
| UniProt ID | Q5JR12 |
| Domains | PP2C |
| Protein Families | Phosphatase |
| MW | 54.8 kDa |
| Gene Summary | This gene encodes the serine/threonine protein phosphatase. The mouse homolog of this gene apparently belongs to the protein phosphatase 2C family of genes. The exact function of this gene is not yet known. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210062L3 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1J (PPM1J), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210062L4 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1J (PPM1J), mGFP tagged |
CNY 5,890.00 |
|
| RG210062 | PPM1J (tGFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1J (PPM1J) |
CNY 4,370.00 |
|
| SC128066 | PPM1J (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1J (PPM1J) |
CNY 3,768.00 |
