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Sec8 (EXOC4) (NM_021807) Human Tagged ORF Clone
CAT＃: RC209487
EXOC4 (Myc-DDK-tagged)-Human exocyst complex component 4 (EXOC4), transcript variant 1
ORF Plasmid: tGFP
"NM_021807" in other vectors (5)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SEC8; SEC8L1; Sec8p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209487 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGAAGCAGCTGGTGGGAAATACAGAAGCACAGTCAGCAAAAGCAAAGACCCCTCGGGGCTGC TCATCTCTGTGATCAGGACTCTGTCTACTAGTGACGATGTCGAAGACAGGGAAAATGAAAAGGGTCGCCT TGAAGAAGCCTACGAGAAATGTGACCGTGACCTGGATGAATTGATTGTACAGCACTACACAGAATTGACG ACAGCCATTCGCACATACCAGAGCATCACAGAGCGCATCACTAACTCCCGAAATAAAATAAAGCAGGTAA AAGAGAACCTGCTTTCATGCAAGATGCTGCTGCACTGCAAACGGGATGAGCTTCGGAAACTGTGGATTGA AGGAATTGAGCATAAGCATGTCCTGAACTTGTTGGATGAAATTGAGAATATCAAGCAAGTGCCTCAAAAG CTGGAACAGTGCATGGCCAGCAAGCACTATCTCAGTGCCACTGACATGTTGGTGTCAGCAGTTGAGTCTT TGGAGGGCCCCCTGCTCCAGGTGGAAGGACTGAGTGACCTTCGACTAGAGCTTCACAGCAAGAAGATGAA CCTTCACTTGGTTCTCATAGATGAACTACACCGGCACCTGTACATCAAATCGACTAGCCGAGTTGTGCAG CGTAACAAGGAAAAAGGGAAAATCAGCTCCCTCGTGAAAGATGCTTCTGTTCCTCTGATTGATGTTACAA ACCTCCCTACTCCTCGAAAATTCCTTGATACCTCTCACTATTCTACTGCTGGAAGCTCAAGTGTGAGGGA GATAAATCTGCAGGACATCAAGGAAGATTTAGAATTGGATCCAGAGGAAAACAGCACCCTGTTTATGGGT ATCCTCATTAAGGGCTTGGCGAAACTGAAGAAGATCCCAGAAACAGTTAAGGCAATCATAGAGCGCTTGG AGCAGGAGTTGAAGCAAATTGTGAAGAGGTCTACAACCCAGGTGGCAGACAGTGGCTATCAGCGGGGGGA GAACGTTACTGTGGAGAACCAACCAAGGTTGCTTCTAGAACTGCTGGAGTTACTGTTTGACAAGTTTAAT GCTGTAGCCGCTGCACACTCTGTGGTCCTGGGATACCTGCAGGACACTGTAGTGACTCCACTGACTCAGC AGGAAGATATCAAACTGTATGATATGGCAGATGTATGGGTGAAGATCCAAGATGTTCTACAGATGCTATT AACTGAGTACTTGGATATGAAAAATACTCGTACGGCCTCTGAACCATCAGCTCAACTAAGCTATGCCAGC ACTGGACGAGAGTTTGCAGCCTTTTTTGCCAAGAAGAAACCTCAAAGGCCAAAAAATTCTCTTTTCAAGT TCGAATCGTCCTCCCATGCCATCAGTATGAGCGCCTATCTGCGAGAACAGAGAAGGGAGCTCTATAGTCG GAGTGGAGAACTGCAAGGGGGTCCTGATGACAACTTACTTGAAGGTGGAGGAACAAAATTTGTCTGCAAA CCTGGAGCCAGAAACATTACCGTCATATTCCACCCATTACTAAGATTTATTCAGGAGATTGAGCATGCTC TGGGTCTTGGCCCAGCCAAACAGTGTCCTCTTCGAGAGTTTCTCACCGTGTACATCAAAAACATCTTTCT CAATCAAGTCTTGGCTGAGATCAACAAGGAGATTGAAGGAGTCACTAAAACATCTGACCCTTTGAAGATT CTGGCCAACGCAGACACCATGAAGGTGCTGGGAGTGCAGCGGCCTCTCCTACAGAGCACAATCATTGTGG AGAAGACAGTTCAAGACCTCCTGAACCTGATGCATGACTTGAGTGCATATTCAGATCAATTCCTCAACAT GGTGTGCGTGAAGCTCCAGGAGTACAAGGACACCTGCACTGCAGCTTACAGGGGTATTGTCCAGTCAGAA GAAAAACTTGTCATCAGTGCATCCTGGGCAAAAGATGATGATATCAGCAGACTCTTGAAATCTCTACCAA ACTGGATGAATATGGCTCAACCCAAACAGCTGAGGCCAAAAAGAGAGGAGGAAGAAGATTTCATAAGGGC AGCTTTTGGCAAGGAGTCTGAAGTTCTTATTGGGAACCTGGGTGATAAATTAATCCCTCCACAAGACATC CTTCGTGACGTCAGTGACCTCAAAGCCTTGGCCAACATGCATGAAAGCCTGGAATGGTTGGCAAGTCGAA CAAAGTCAGCTTTCTCCAATCTTTCTACATCCCAGATGCTTTCTCCTGCTCAAGACAGCCACACGAACAC GGATCTCCCCCCAGTGTCAGAGCAGATCATGCAGACTCTCAGTGAACTTGCCAAATCGTTCCAGGATATG GCTGACCGCTGCTTGCTTGTCTTACATCTGGAAGTGAGGGTTCACTGTTTCCACTATCTTATCCCTCTTG CAAAGGAGGGGAACTATGCCATTGTGGCTAATGTGGAAAGTATGGATTATGACCCCCTGGTGGTCAAGCT CAACAAAGATATCAGCGCCATTGAAGAGGCCATGAGCGCCAGCCTTCAGCAGCACAAGTTCCAGTATATC TTCGAAGGCCTGGGCCACCTGATCTCCTGCATCCTCATTAATGGTGCCCAGTACTTCAGGCGCATCAGTG AGTCTGGCATCAAGAAAATGTGTAGGAACATTTTTGTTCTTCAGCAGAATTTGACCAACATCACCATGTC GCGGGAGGCAGACCTGGACTTTGCAAGGCAGTACTACGAGATGCTTTACAACACAGCTGACGAGCTCCTG AACCTGGTGGTGGACCAGGGTGTGAAGTACACGGAGCTGGAGTACATCCACGCTCTGACCCTGCTGCACC GCAGCCAAACTGGGGTGGGGGAACTGACCACCCAGAACACGAGGCTGCAGAGGCTCAAAGAGATCATCTG CGAGCAGGCTGCCATCAAGCAAGCCACCAAGGACAAGAAGATAACTACCGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209487 protein sequence
Red=Cloning site Green=Tags(s) MAAEAAGGKYRSTVSKSKDPSGLLISVIRTLSTSDDVEDRENEKGRLEEAYEKCDRDLDELIVQHYTELT TAIRTYQSITERITNSRNKIKQVKENLLSCKMLLHCKRDELRKLWIEGIEHKHVLNLLDEIENIKQVPQK LEQCMASKHYLSATDMLVSAVESLEGPLLQVEGLSDLRLELHSKKMNLHLVLIDELHRHLYIKSTSRVVQ RNKEKGKISSLVKDASVPLIDVTNLPTPRKFLDTSHYSTAGSSSVREINLQDIKEDLELDPEENSTLFMG ILIKGLAKLKKIPETVKAIIERLEQELKQIVKRSTTQVADSGYQRGENVTVENQPRLLLELLELLFDKFN AVAAAHSVVLGYLQDTVVTPLTQQEDIKLYDMADVWVKIQDVLQMLLTEYLDMKNTRTASEPSAQLSYAS TGREFAAFFAKKKPQRPKNSLFKFESSSHAISMSAYLREQRRELYSRSGELQGGPDDNLLEGGGTKFVCK PGARNITVIFHPLLRFIQEIEHALGLGPAKQCPLREFLTVYIKNIFLNQVLAEINKEIEGVTKTSDPLKI LANADTMKVLGVQRPLLQSTIIVEKTVQDLLNLMHDLSAYSDQFLNMVCVKLQEYKDTCTAAYRGIVQSE EKLVISASWAKDDDISRLLKSLPNWMNMAQPKQLRPKREEEEDFIRAAFGKESEVLIGNLGDKLIPPQDI LRDVSDLKALANMHESLEWLASRTKSAFSNLSTSQMLSPAQDSHTNTDLPPVSEQIMQTLSELAKSFQDM ADRCLLVLHLEVRVHCFHYLIPLAKEGNYAIVANVESMDYDPLVVKLNKDISAIEEAMSASLQQHKFQYI FEGLGHLISCILINGAQYFRRISESGIKKMCRNIFVLQQNLTNITMSREADLDFARQYYEMLYNTADELL NLVVDQGVKYTELEYIHALTLLHRSQTGVGELTTQNTRLQRLKEIICEQAAIKQATKDKKITTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021807 |
| ORF Size | 2922 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021807.4 |
| RefSeq Size | 4203 bp |
| RefSeq ORF | 2925 bp |
| Locus ID | 60412 |
| UniProt ID | Q96A65 |
| Domains | Sec8_exocyst |
| Protein Pathways | Tight junction |
| MW | 110.5 kDa |
| Gene Summary | The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
SmartBac, a new baculovirus system for large protein complex production
,Zhai, Y;Zhang, D;Yu, L;Sun, F;Sun, F;,
Journal of Structural Biology: X
[Sec8]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209487L3 | Lenti ORF clone of Human exocyst complex component 4 (EXOC4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209487L4 | Lenti ORF clone of Human exocyst complex component 4 (EXOC4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG209487 | EXOC4 (tGFP-tagged) - Human exocyst complex component 4 (EXOC4), transcript variant 1 |
CNY 4,370.00 |
|
| SC102359 | EXOC4 (untagged)-Human exocyst complex component 4 (EXOC4), transcript variant 1 |
CNY 9,584.00 |
|
| SC323875 | EXOC4 (untagged)-Human exocyst complex component 4 (EXOC4), transcript variant 1 |
CNY 7,144.00 |
