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Apc4 (ANAPC4) (NM_013367) Human Tagged ORF Clone
CAT＃: RC209242
- TrueORF®
ANAPC4 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 4 (ANAPC4)
ORF Plasmid: tGFP
"NM_013367" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | APC4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209242 representing NM_013367
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCGTTTTCCGACCTGTTTCCCATCCTTCCGGGTGGTGGGAGAGAAGCAGCTCCCGCAGGAGATTA TTTTCCTGGTCTGGTCGCCCAAGCGGGATCTCATTGCTTTGGCCAACACAGCTGGCGAGGTTTTACTTCA TCGACTGGCAAGTTTTCATCGAGTTTGGAGTTTTCCACCAAATGAAAATACAGGAAAGGAGGTGACGTGT CTGGCATGGAGACCAGATGGCAAACTTTTGGCCTTTGCTCTTGCTGATACCAAGAAAATTGTTTTGTGTG ATGTAGAAAAACCTGAGAGCTTACACTCTTTTTCTGTGGAGGCTCCAGTTTCCTGTATGCATTGGATGGA AGTGACAGTAGAAAGCAGTGTTCTCACATCATTTTATAATGCTGAGGATGAATCAAATCTTCTCTTACCT AAACTACCTACACTGCCAAAAAACTATAGCAACACCTCAAAAATATTTAGTGAAGAAAATTCTGATGAAA TTATTAAGCTCTTGGGAGACGTCAGGCTTAATATTCTCGTCCTTGGAGGAAGCTCTGGATTTATTGAGCT TTATGCTTATGGAATGTTTAAAATTGCTCGAGTCACAGGGATTGCTGGTACTTGTCTTGCATTATGTTTA TCAAGTGATTTGAAATCATTATCAGTGGTCACAGAAGTCTCTACCAATGGTGCTTCAGAAGTTTCATACT TTCAGCTTGAAACTAATCTGTTGTACTCTTTCTTACCTGAAGTAACTCGGATGGCCAGAAAGTTTACTCA TATTTCAGCTCTGTTACAGTATATAAATTTGTCACTAACATGTATGTGTGAAGCATGGGAAGAAATACTA ATGCAGATGGATTCTCGTCTCACCAAGTTTGTGCAGGAAAAGAACACAACCACATCAGTGCAAGATGAGT TCATGCACTTGCTATTATGGGGGAAAGCAAGTGCTGAACTTCAGACTCTCTTGATGAATCAGTTAACAGT AAAGGGCTTGAAAAAGCTTGGCCAGTCTATAGAGTCATCATACTCCAGTATACAAAAATTGGTCATAAGT CATTTACAGAGTGGCTCGGAGTCTTTATTATACCATTTGAGTGAATTGAAAGGAATGGCTTCATGGAAGC AAAAATATGAACCTCTTGGACTAGATGCTGCAGGAATCGAAGAAGCTATAACTGCTGTGGGTTCTTTTAT ACTCAAGGCAAATGAACTTCTTCAAGTTATAGATAGTAGTATGAAAAACTTCAAAGCATTTTTTCGGTGG CTTTATGTGGCAATGTTGAGAATGACAGAAGACCATGTGCTTCCCGAGCTGAATAAGATGACTCAGAAAG ATATCACATTTGTTGCTGAATTTCTTACTGAACATTTCAATGAGGCTCCAGACCTTTATAATCGAAAAGG AAAATACTTTAACGTTGAAAGAGTTGGTCAGTACTTGAAAGATGAAGATGATGATCTTGTGTCACCCCCT AACACAGAAGGAAACCAGTGGTATGACTTTCTTCAAAATAGCAGCCACCTTAAAGAAAGTCCTTTGCTGT TTCCTTATTATCCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAATATTATTGATCAGTGTTT GCAAAAGCCAGCAGATGTAATTGGAAAATCGATGAATCAAGCAATCTGTATTCCATTGTATAGAGATACC AGAAGTGAGGATTCTACACGTAGATTGTTCAAATTTCCTTTTCTGTGGAATAATAAAACTTCAAATCTAC ATTATCTTCTTTTTACTATTCTAGAAGATTCACTTTATAAAATGTGCATCTTAAGGAGACATACTGATAT TTCTCAATCTGTGAGTAATGGACTAATTGCTATTAAATTTGGGAGCTTTACATATGCCACAACAGAAAAA GTCAGAAGAAGCATCTACAGTTGTTTAGATGCACAGTTTTATGATGATGAAACTGTAACAGTAGTTCTTA AAGACACTGTAGGACGTGAAGGAAGAGATAGACTCTTGGTCCAGCTGCCTTTGTCTTTAGTATATAACAG TGAAGATTCTGCAGAATATCAGTTCACTGGGACTTATTCTACAAGGCTAGATGAACAGTGTAGTGCTATT CCCACCCGTACCATGCATTTCGAGAAGCACTGGAGATTACTGGAAAGTATGAAAGCACAGTATGTTGCTG GGAATGGTTTTCGAAAAGTGTCCTGTGTGTTAAGCTCAAATCTTCGTCATGTGAGAGTATTTGAAATGGA CATAGATGATGAATGGGAGCTCGATGGGTCTTCAGATGAAGAGGAGGAGGCCAGTAATAAGCCTGTAAAA ATAAAGGAAGAAGTGTTGTCGGAGTCAGAGGCAGAGAACCAACAAGCTGGTGCTGCCGCTTTAGCTCCAG AGATAGTCATTAAAGTGGAAAAACTTGACCCTGAGCTAGACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209242 representing NM_013367
Red=Cloning site Green=Tags(s) MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTC LAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLP KLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCL SSDLKSLSVVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEIL MQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVIS HLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEEAITAVGSFILKANELLQVIDSSMKNFKAFFRW LYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPP NTEGNQWYDFLQNSSHLKESPLLFPYYPRKSLHFVKRRMENIIDQCLQKPADVIGKSMNQAICIPLYRDT RSEDSTRRLFKFPFLWNNKTSNLHYLLFTILEDSLYKMCILRRHTDISQSVSNGLIAIKFGSFTYATTEK VRRSIYSCLDAQFYDDETVTVVLKDTVGREGRDRLLVQLPLSLVYNSEDSAEYQFTGTYSTRLDEQCSAI PTRTMHFEKHWRLLESMKAQYVAGNGFRKVSCVLSSNLRHVRVFEMDIDDEWELDGSSDEEEEASNKPVK IKEEVLSESEAENQQAGAAALAPEIVIKVEKLDPELDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013367 |
| ORF Size | 2424 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013367.3 |
| RefSeq Size | 2701 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 29945 |
| UniProt ID | Q9UJX5 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
| MW | 91.9 kDa |
| Gene Summary | A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209242L1 | Lenti-ORF clone of ANAPC4 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 4 (ANAPC4) |
CNY 5,890.00 |
|
| RC209242L2 | Lenti-ORF clone of ANAPC4 (mGFP-tagged)-Human anaphase promoting complex subunit 4 (ANAPC4) |
CNY 5,890.00 |
|
| RC209242L3 | Lenti-ORF clone of ANAPC4 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 4 (ANAPC4) |
CNY 5,890.00 |
|
| RC209242L4 | Lenti-ORF clone of ANAPC4 (mGFP-tagged)-Human anaphase promoting complex subunit 4 (ANAPC4) |
CNY 11,272.00 |
|
| RG209242 | ANAPC4 (tGFP-tagged) - Human anaphase promoting complex subunit 4 (ANAPC4) |
CNY 10,472.00 |
|
| SC310100 | ANAPC4 (untagged)-Human anaphase promoting complex subunit 4 (ANAPC4) |
CNY 7,220.00 |
