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BYSL (NM_004053) Human Tagged Lenti ORF Clone
CAT＃: RC208675L4
- LentiORF®
Lenti ORF clone of Human bystin-like (BYSL), mGFP tagged
"NM_004053" in other vectors (6)
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CNY 5,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | BYSTIN; Enp1 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC208675).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004053 |
| ORF Size | 1311 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004053.3 |
| RefSeq Size | 2005 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 705 |
| UniProt ID | Q13895 |
| Protein Families | Stem cell - Pluripotency |
| MW | 49.6 kDa |
| Gene Summary | Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208675 | BYSL (Myc-DDK-tagged)-Human bystin-like (BYSL) |
CNY 3,656.00 |
|
| RC208675L1 | Lenti ORF clone of Human bystin-like (BYSL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208675L2 | Lenti ORF clone of Human bystin-like (BYSL), mGFP tagged |
CNY 5,890.00 |
|
| RC208675L3 | Lenti ORF clone of Human bystin-like (BYSL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RG208675 | BYSL (tGFP-tagged) - Human bystin-like (BYSL) |
CNY 4,370.00 |
|
| SC117600 | BYSL (untagged)-Human bystin-like (BYSL) |
CNY 2,950.00 |
