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MAN2C1 (NM_006715) Human Tagged ORF Clone
CAT＃: RC208517
MAN2C1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2C, member 1 (MAN2C1)
ORF Plasmid: tGFP
"NM_006715" in other vectors (5)
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CNY 7,240.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MAN6A8; MANA; MANA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208517 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGCGCCGGCCTTGAAGCACTGGCGCACCACGCTGGAGCGGGTGGAGAAGTTCGTGTCGCCGC TCTACTTTACCGACTGTAACCTCCGCGGCAGGCTTTTTGGGGCCAGCTGCCCTGTGGCTGTGCTCTCCAG CTTCCTGACGCCGGAGAGACTTCCCTACCAGGAGGCAGTCCAGCGGGACTTCCGCCCCGCGCAGGTCGGC GACAGCTTCGGACCCACATGGTGGACCTGCTGGTTCCGGGTGGAGCTGACCATCCCAGAGGCATGGGTGG GCCAGGAAGTTCACCTTTGCTGGGAAAGTGATGGAGAAGGTCTGGTGTGGCGTGATGGAGAACCTGTCCA GGGTTTAACCAAAGAGGGTGAGAAGACCAGCTATGTCCTGACTGACAGGCTGGGGGAAAGAGACCCCCGA AGCCTCACTCTCTATGTGGAAGTAGCCTGCAATGGGCTCCTGGGGGCCGGGAAGGGAAGCATGATTGCAG CCCCTGACCCTGAGAAGATGTTCCAGCTGAGCCGGGCTGAGCTAGCTGTGTTCCACCGGGATGTCCACAT GCTCCTGGTGGATCTGGAGCTGCTGCTGGGCATAGCCAAGGGCCTCGGGAAGGACAACCAGCGCAGCTTC CAGGCCCTGTACACAGCCAATCAGATGGTGAACGTGTGTGACCCTGCCCAGCCCGAGACCTTCCCAGTGG CCCAGGCCCTGGCCTCCAGGTTCTTTGGCCAACATGGGGGTGAAAGCCAACACACCATTCATGCCACAGG GCACTGCCACATTGATACAGCCTGGCTTTGGCCCTTCAAAGAGACTGTGAGGAAATGTGCCCGGAGCTGG GTGACCGCCCTGCAGCTCATGGAGCGGAACCCTGAGTTCATCTTTGCCTGCTCCCAGGCGCAGCAGCTGG AATGGGTGAAGAGCCGCTACCCTGGCCTGTACTCCCGCATCCAGGAGTTTGCGTGCCGTGGGCAGTTTGT GCCTGTGGGGGGCACCTGGGTGGAGATGGATGGGAACCTGCCCAGTGGAGAGGCCATGGTGAGGCAGTTT TTGCAGGGCCAGAACTTCTTTCTGCAGGAGTTTGGGAAGATGTGCTCTGAGTTCTGGCTGCCGGACACCT TTGGCTACTCAGCACAGCTCCCCCAGATCATGCACGGCTGTGGCATCAGGCGCTTTCTCACCCAGAAATT GAGCTGGAATTTGGTGAACTCCTTCCCACACCATACATTTTTCTGGGAGGGCCTGGATGGCTCCCGTGTA CTGGTCCACTTCCCACCTGGCGACTCCTATGGGATGCAGGGCAGCGTGGAGGAGGTGCTGAAGACCGTGG CCAACAACCGGGACAAGGGGCGGGCCAACCACAGTGCCTTCCTCTTTGGCTTTGGGGATGGGGGTGGTGG CCCCACCCAGACCATGCTGGACCGCCTGAAGCGCCTGAGCAATACGGATGGGCTGCCCAGGGTGCAGCTA TCTTCTCCAAGACAGCTCTTCTCAGCACTGGAGAGTGACTCAGAGCAGCTGTGCACGTGGGTTGGGGAGC TCTTCTTGGAGCTGCACAATGGCACATACACCACCCATGCCCAGATCAAGAAGGGGAACCGGGAATGTGA GCGGATCCTGCACGACGTGGAGCTGCTCAGTAGCCTGGCCCTGGCCCGCAGTGCCCAGTTCCTATACCCA GCAGCCCAGCTGCAGCACCTCTGGAGGCTCCTTCTTCTGAACCAGTTCCATGATGTGGTGACTGGAAGCT GCATCCAGATGGTGGCAGAGGAAGCCATGTGCCATTATGAAGACATCCGTTCCCATGGCAATACACTGCT CAGCGCTGCAGCCGCAGCCCTGTGTGCTGGGGAGCCAGGTCCTGAGGGCCTCCTCATCGTCAACACACTG CCCTGGAAGCGGATCGAAGTGATGGCCCTGCCCAAACCGGGCGGGGCCCACAGCCTAGCCCTGGTGACAG TGCCCAGCATGGGCTATGCTCCTGTTCCTCCCCCCACCTCACTGCAGCCCCTGCTGCCCCAGCAGCCTGT GTTCGTAGTGCAAGAGACTGATGGCTCCGTGACTCTGGACAATGGCATCATCCGAGTGAAGCTGGACCCA ACTGGTCGCCTGACGTCCTTGGTCCTGGTGGCCTCTGGCAGGGAGGCCATTGCTGAGGGCGCCGTGGGGA ACCAGTTTGTGCTATTTGATGATGTCCCCTTGTACTGGGATGCATGGGACGTCATGGACTACCACCTGGA GACACGGAAGCCTGTGCTGGGCCAGGCAGGGACCCTGGCAGTGGGCACCGAGGGCGGCCTGCGGGGCAGC GCCTGGTTCTTGCTACAGATCAGCCCCAACAGTCGGCTTAGCCAGGAGGTTGTGCTGGACGTTGGCTGCC CCTATGTCCGCTTCCACACCGAGGTACACTGGCATGAGGCCCACAAGTTCCTGAAGGTGGAGTTCCCTGC TCGCGTGCGGAGTTCCCAGGCCACCTATGAGATCCAGTTTGGGCACCTGCAGCGACCTACCCACTACAAT ACCTCTTGGGACTGGGCTCGATTTGAGGTGTGGGCCCATCGCTGGATGGATCTGTCAGAACACGGCTTTG GGCTGGCCCTGCTCAACGACTGCAAGTATGGCGCGTCAGTGCGAGGCAGCATCCTCAGCCTCTCGCTCTT GCGGGCGCCTAAAGCCCCGGACGCTACTGCTGACACGGGGCGCCACGAGTTCACCTATGCACTGATGCCG CACAAGGGCTCTTTCCAGGATGCTGGCGTTATCCAAGCTGCCTACAGCCTAAACTTCCCCCTGTTGGCTC TGCCAGCCCCCAGCCCAGCGCCCGCCACCTCCTGGAGTGCGTTTTCCGTGTCTTCACCCGCGGTCGTATT GGAGACCGTCAAGCAGGCGGAGAGCAGCCCCCAGCGCCGCTCGCTGGTCCTGAGGCTGTATGAGGCCCAC GGCAGCCACGTGGACTGCTGGCTGCACTTGTCGCTGCCGGTTCAGGAGGCCATCCTCTGCGATCTCTTGG AGCGACCAGACCCTGCTGGCCACTTGACCCTTCGGGACAACCGCCTGAAGCTCACCTTTTCTCCCTTCCA AGTGCTGTCCCTGTTGCTCGTGCTTCAGCCTCCGCCACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208517 protein sequence
Red=Cloning site Green=Tags(s) MAAAPALKHWRTTLERVEKFVSPLYFTDCNLRGRLFGASCPVAVLSSFLTPERLPYQEAVQRDFRPAQVG DSFGPTWWTCWFRVELTIPEAWVGQEVHLCWESDGEGLVWRDGEPVQGLTKEGEKTSYVLTDRLGERDPR SLTLYVEVACNGLLGAGKGSMIAAPDPEKMFQLSRAELAVFHRDVHMLLVDLELLLGIAKGLGKDNQRSF QALYTANQMVNVCDPAQPETFPVAQALASRFFGQHGGESQHTIHATGHCHIDTAWLWPFKETVRKCARSW VTALQLMERNPEFIFACSQAQQLEWVKSRYPGLYSRIQEFACRGQFVPVGGTWVEMDGNLPSGEAMVRQF LQGQNFFLQEFGKMCSEFWLPDTFGYSAQLPQIMHGCGIRRFLTQKLSWNLVNSFPHHTFFWEGLDGSRV LVHFPPGDSYGMQGSVEEVLKTVANNRDKGRANHSAFLFGFGDGGGGPTQTMLDRLKRLSNTDGLPRVQL SSPRQLFSALESDSEQLCTWVGELFLELHNGTYTTHAQIKKGNRECERILHDVELLSSLALARSAQFLYP AAQLQHLWRLLLLNQFHDVVTGSCIQMVAEEAMCHYEDIRSHGNTLLSAAAAALCAGEPGPEGLLIVNTL PWKRIEVMALPKPGGAHSLALVTVPSMGYAPVPPPTSLQPLLPQQPVFVVQETDGSVTLDNGIIRVKLDP TGRLTSLVLVASGREAIAEGAVGNQFVLFDDVPLYWDAWDVMDYHLETRKPVLGQAGTLAVGTEGGLRGS AWFLLQISPNSRLSQEVVLDVGCPYVRFHTEVHWHEAHKFLKVEFPARVRSSQATYEIQFGHLQRPTHYN TSWDWARFEVWAHRWMDLSEHGFGLALLNDCKYGASVRGSILSLSLLRAPKAPDATADTGRHEFTYALMP HKGSFQDAGVIQAAYSLNFPLLALPAPSPAPATSWSAFSVSSPAVVLETVKQAESSPQRRSLVLRLYEAH GSHVDCWLHLSLPVQEAILCDLLERPDPAGHLTLRDNRLKLTFSPFQVLSLLLVLQPPPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006715 |
| ORF Size | 3120 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006715.4 |
| RefSeq Size | 3291 bp |
| RefSeq ORF | 3123 bp |
| Locus ID | 4123 |
| UniProt ID | Q9NTJ4 |
| Domains | Glyco_hydro_38 |
| Protein Families | Druggable Genome |
| Protein Pathways | Other glycan degradation |
| MW | 115.8 kDa |
| Gene Summary | Cleaves alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues from glycoproteins. Involved in the degradation of free oligosaccharides in the cytoplasm.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208517L3 | Lenti ORF clone of Human mannosidase, alpha, class 2C, member 1 (MAN2C1), Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC208517L4 | Lenti ORF clone of Human mannosidase, alpha, class 2C, member 1 (MAN2C1), mGFP tagged |
CNY 7,600.00 |
|
| RG208517 | MAN2C1 (tGFP-tagged) - Human mannosidase, alpha, class 2C, member 1 (MAN2C1) |
CNY 6,270.00 |
|
| SC110944 | MAN2C1 (untagged)-Human mannosidase, alpha, class 2C, member 1 (MAN2C1) |
CNY 7,248.00 |
|
| SC324329 | MAN2C1 (untagged)-Human mannosidase, alpha, class 2C, member 1 (MAN2C1) |
CNY 7,248.00 |
