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PANK4 (NM_018216) Human Tagged ORF Clone
CAT＃: RC208116
- TrueORF®
PANK4 (Myc-DDK-tagged)-Human pantothenate kinase 4 (PANK4)
ORF Plasmid: tGFP
"NM_018216" in other vectors (4)
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CNY 5,664.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208116 representing NM_018216
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGTGTGGAGCGAGCGGCAGCGGGAGCAGCGGGGACAGTCTGGACAAGAGCATCACGCTGCCCC CCGACGAGATCTTCCGCAACCTGGAGAACGCCAAGCGCTTCGCCATCGACATAGGCGGGTCGTTAACCAA GCTGGCCTACTATTCAACGGTACAGCACAAAGTCGCCAAGGTGCGGTCTTTCGACCACTCCGGAAAGGAC ACAGAACGTGAACATGAGCCGCCCTATGAGATTTCAGTTCAAGAAGAGATCACTGCTCGACTGCACTTCA TTAAGTTTGAGAATACCTACATCGAAGCCTGCCTGGACTTCATCAAAGACCATCTCGTCAACACAGAGAC CAAGGTCATCCAGGCGACCGGGGGCGGGGCCTACAAGTTCAAGGACCTCATCGAAGAGAAGCTGCGGCTG AAAGTCGACAAGGAGGACGTGATGACGTGCCTGATTAAGGGGTGCAACTTCGTGCTCAAGAACATCCCCC ATGAGGCCTTCGTGTACCAGAAGGATTCCGACCCTGAGTTCCGGTTCCAGACCAACCACCCCCACATTTT CCCCTATCTTCTTGTCAATATCGGCTCTGGAGTCTCCATCGTGAAGGTGGAGACGGAGGACAGGTTCGAG TGGGTCGGCGGCAGCTCCATTGGAGGCGGCACCTTCTGGGGGCTTGGCGCTCTGCTCACCAAAACGAAGA AGTTTGACGAGCTCCTGCACCTGGCCTCGAGGGGCCAGCACAGCAATGTGGACATGCTGGTGCGGGACGT CTACGGCGGCGCCCACCAGACTCTCGGGCTGAGCGGGAACCTCATCGCCAGCAGCTTCGGGAAGTCGGCC ACCGCCGACCAAGAGTTCTCCAAAGAAGACATGGCGAAGAGCCTGCTGCACATGATCAGCAACGACATTG GGCAGCTGGCCTGCCTCCACGCACGGCTGCACAGCCTGGACCGCGTGTACTTTGGAGGCTTCTTTATCCG GGGCCACCCCGTGACCATGCGCACCATCACCTATAGCATCAACTTCTTCTCCAAGGGGGAAGTGCAGGCG CTGTTTCTGAGGCACGAAGGCTACCTGGGAGCCATCGGAGCGTTCCTGAAAGGAGCTGAGCAGGACAATC CTAACCAGTACAGCTGGGGAGAGAACTATGCAGGCAGCTCCGGGCTGATGAGTGCATCACCCGAGCTCGG CCCGGCGCAGCGGGCGCGGAGTGGCACTTTTGACTTGCTGGAAATGGACCGGCTGGAGAGGCCACTGGTT GACCTGCCGCTCCTCCTGGACCCGCCCTCCTACGTGCCCGACACGGTGGACCTCACCGATGACGCTCTGG CCCGAAAATACTGGCTCACCTGCTTTGAGGAGGCCCTGGACGGGGTAGTGAAGCGCGCAGTGGCGAGCCA GCCAGACTCTGTGGATGCAGCCGAGAGGGCGGAGAAGTTCCGGCAGAAGTACTGGAACAAGCTTCAGACC CTGAGGCAGCAGCCCTTCGCCTATGGGACCCTGACCGTGCGCAGCCTGCTGGACACCAGGGAGCACTGTC TGAACGAGTTCAACTTCCCGGATCCCTACTCCAAAGTGAAGCAGCGGGAGAATGGCGTGGCGCTGAGGTG CTTCCCCGGGGTCGTGCGCTCCCTGGACGCGCTGGGCTGGGAGGAACGGCAGCTGGCGCTGGTGAAAGGC CTCCTGGCGGGGAATGTCTTCGACTGGGGGGCCAAAGCCGTGTCTGCTGTCCTTGAATCCGACCCCTACT TTGGGTTTGAAGAAGCAAAGAGGAAGTTACAAGAAAGACCCTGGCTCGTGGATTCCTACAGCGAGTGGCT TCAGAGATTAAAGGGGCCCCCTCATAAATGTGCCTTAATTTTCGCAGATAACAGTGGAATAGACATCATT TTGGGAGTCTTCCCCTTTGTCAGGGAGCTACTCCTTAGAGGGACAGAGGTCATCCTGGCGTGCAACTCAG GCCCCGCCCTGAACGACGTGACCCACAGCGAGTCCCTCATCGTGGCAGAGCGTATTGCGGGCATGGACCC TGTCGTGCACTCTGCGCTCCAGGAAGAGAGGCTGCTGCTGGTGCAGACGGGCTCCAGCTCCCCGTGCCTC GACCTCAGCCGCCTGGATAAGGGGCTGGCCGCACTGGTGCGGGAGCGTGGCGCGGATCTGGTGGTCATCG AGGGCATGGGCCGTGCTGTCCACACAAACTACCACGCAGCCCTGCGCTGCGAGAGCCTCAAGCTGGCCGT CATCAAGAACGCGTGGCTGGCCGAGCGGCTGGGCGGCCGGCTCTTCAGCGTCATCTTCAAGTACGAGGTC CCAGCCGAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC208116 representing NM_018216
Red=Cloning site Green=Tags(s) MAECGASGSGSSGDSLDKSITLPPDEIFRNLENAKRFAIDIGGSLTKLAYYSTVQHKVAKVRSFDHSGKD TEREHEPPYEISVQEEITARLHFIKFENTYIEACLDFIKDHLVNTETKVIQATGGGAYKFKDLIEEKLRL KVDKEDVMTCLIKGCNFVLKNIPHEAFVYQKDSDPEFRFQTNHPHIFPYLLVNIGSGVSIVKVETEDRFE WVGGSSIGGGTFWGLGALLTKTKKFDELLHLASRGQHSNVDMLVRDVYGGAHQTLGLSGNLIASSFGKSA TADQEFSKEDMAKSLLHMISNDIGQLACLHARLHSLDRVYFGGFFIRGHPVTMRTITYSINFFSKGEVQA LFLRHEGYLGAIGAFLKGAEQDNPNQYSWGENYAGSSGLMSASPELGPAQRARSGTFDLLEMDRLERPLV DLPLLLDPPSYVPDTVDLTDDALARKYWLTCFEEALDGVVKRAVASQPDSVDAAERAEKFRQKYWNKLQT LRQQPFAYGTLTVRSLLDTREHCLNEFNFPDPYSKVKQRENGVALRCFPGVVRSLDALGWEERQLALVKG LLAGNVFDWGAKAVSAVLESDPYFGFEEAKRKLQERPWLVDSYSEWLQRLKGPPHKCALIFADNSGIDII LGVFPFVRELLLRGTEVILACNSGPALNDVTHSESLIVAERIAGMDPVVHSALQEERLLLVQTGSSSPCL DLSRLDKGLAALVRERGADLVVIEGMGRAVHTNYHAALRCESLKLAVIKNAWLAERLGGRLFSVIFKYEV PAE SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018216 |
| ORF Size | 2319 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018216.1, NP_060686.1 |
| RefSeq Size | 2642 bp |
| RefSeq ORF | 2322 bp |
| Locus ID | 55229 |
| UniProt ID | Q9NVE7 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Pantothenate and CoA biosynthesis |
| MW | 85.99 kDa |
| Gene Summary | This gene encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is most abundant in muscle but is expressed in all tissues. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208116L3 | Lenti-ORF clone of PANK4 (Myc-DDK-tagged)-Human pantothenate kinase 4 (PANK4) |
CNY 7,700.00 |
|
| RC208116L4 | Lenti-ORF clone of PANK4 (mGFP-tagged)-Human pantothenate kinase 4 (PANK4) |
CNY 7,700.00 |
|
| RG208116 | PANK4 (tGFP-tagged) - Human pantothenate kinase 4 (PANK4) |
CNY 6,370.00 |
|
| SC113651 | PANK4 (untagged)-Human pantothenate kinase 4 (PANK4) |
CNY 5,672.00 |
