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Adducin 2 (ADD2) (NM_017488) Human Tagged ORF Clone
CAT＃: RC207895
ADD2 (Myc-DDK-tagged)-Human adducin 2 (beta) (ADD2), transcript variant 4
ORF Plasmid: tGFP
"NM_017488" in other vectors (4)
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CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADDB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207895 representing NM_017488
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAAGAGACGGTCCCCGAGGCTGCCTCGCCGCCGCCCCCGCAGGGGCAGCCTTACTTTGACCGCT TCTCAGAGGACGACCCCGAGTACATGCGCCTTCGCAACCGGGCGGCGGACCTGCGGCAGGACTTCAACCT GATGGAGCAGAAGAAGCGCGTCACCATGATCCTGCAGAGTCCCTCTTTCAGGGAGGAGCTGGAAGGCCTC ATCCAGGAGCAGATGAAGAAGGGGAACAACTCCTCCAACATCTGGGCCCTGCGACAGATCGCGGACTTCA TGGCCAGCACCTCCCACGCAGTCTTCCCGACATCTTCCATGAATGTCTCCATGATGACGCCTATCAATGA CCTCCACACAGCTGACTCCCTGAACCTGGCCAAAGGGGAGCGGCTCATGCGGTGCAAGATCAGCAGTGTC TACCGACTCCTGGACCTCTATGGCTGGGCCCAGCTGAGTGACACCTATGTCACGTTGAGAGTCAGCAAGG AGCAGGACCACTTCCTGATCAGCCCTAAGGGAGTTTCTTGCAGTGAAGTCACAGCGTCCAGCCTGATCAA GGTGAACATTCTGGGAGAGGTGGTGGAGAAGGGCAGCAGCTGCTTCCCAGTGGACACCACAGGCTTCTGT CTGCACTCGGCCATCTATGCAGCGAGGCCCGACGTGCGCTGCATCATCCACCTGCACACACCGGCCACAG CAGCGGTGTCGGCCATGAAGTGGGGCCTCCTGCCTGTCTCCCACAATGCCCTGCTGGTGGGGGACATGGC CTATTATGACTTCAATGGGGAAATGGAGCAGGAAGCCGATCGGATCAACCTGCAGAAGTGCCTTGGACCC ACCTGCAAGATCCTGGTGCTAAGAAACCATGGAGTGGTTGCTCTGGGTGACACGGTAGAGGAGGCATTTT ACAAGATCTTCCACCTGCAGGCTGCATGTGAGATACAGGTGTCGGCTCTGTCCAGTGCCGGGGGAGTGGA GAACCTCATCCTCCTGGAGCAGGAGAAGCACCGGCCCCATGAGGTGGGCTCCGTGCAGTGGGCCGGGAGC ACCTTTGGGCCTATGCAGAAGAGTCGGCTGGGGGAGCATGAGTTTGAGGCCCTCATGAGGATGCTGGACA ACCTGGGCTACAGAACAGGTTACACGTATCGCCACCCCTTTGTTCAAGAGAAAACCAAACACAAAAGTGA GGTGGAGATTCCAGCCACGGTCACAGCCTTCGTGTTTGAGGAGGACGGTGCCCCGGTGCCCGCCCTGCGA CAGCATGCCCAGAAGCAGCAGAAGGAGAAGACCCGCTGGCTCAATACGCCCAACACCTACCTGCGGGTCA ATGTGGCCGATGAGGTCCAGAGGAGCATGGGCAGCCCCCGACCCAAGACCACGTGGATGAAGGCTGACGA GGTGGAGAAATCCAGCAGTGGCATGCCGATTCGCATCGAAAACCCAAACCAATTTGTGCCTCTCTATACT GACCCCCAGGAAGTACTGGAGATGAGGAACAAGATTCGAGAACAAAACCGACAAGATGTGAAGTCAGCGG GGCCTCAGTCCCAGCTCCTGGCGAGCGTCATTGCCGAGAAGAGCCGAAGCCCGTCTACAGAGAGCCAGCT GATGTCCAAGGGAGACGAGGATACCAAAGACGATTCAGAGGAGACGGTGCCCAACCCCTTCAGCCAACTC ACTGACCAGGAGTTGGAGGAGTACAAGAAAGAGGTGGAGAGGAAGAAACTAGAACTTGATGAGACAGGAC AGGAACGAGAGCCAGGCTCTGGTCCGGCCGTGTGCGAGTTCTTCAGCGTTGCCCTCCACATCTGGAGTAA CATATTGGAGAGAAAGAAACTGCCCCAGAAGAGCCTGGCTCACCTGCAAAGTCTGCACCTGCTTCTCCAG TGCAGAGCCCAGCGAAGGAGGCAGAGACAAAGAGCCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207895 representing NM_017488
Red=Cloning site Green=Tags(s) MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGL IQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSV YRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFC LHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGP TCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQEKHRPHEVGSVQWAGS TFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGAPVPALR QHAQKQQKEKTRWLNTPNTYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYT DPQEVLEMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQL TDQELEEYKKEVERKKLELDETGQEREPGSGPAVCEFFSVALHIWSNILERKKLPQKSLAHLQSLHLLLQ CRAQRRRQRQRAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017488 |
| ORF Size | 1929 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017488.4 |
| RefSeq Size | 4043 bp |
| RefSeq ORF | 1932 bp |
| Locus ID | 119 |
| UniProt ID | P35612 |
| MW | 72.5 kDa |
| Gene Summary | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207895L3 | Lenti ORF clone of Human adducin 2 (beta) (ADD2), transcript variant 4, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207895L4 | Lenti ORF clone of Human adducin 2 (beta) (ADD2), transcript variant 4, mGFP tagged |
CNY 6,460.00 |
|
| RG207895 | ADD2 (tGFP-tagged) - Human adducin 2 (beta) (ADD2), transcript variant 4 |
CNY 5,040.00 |
|
| SC314313 | ADD2 (untagged)-Human adducin 2 (beta) (ADD2), transcript variant 4 |
CNY 7,220.00 |
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