Steroidogenic Factor 1 (NR5A1) (NM_004959) Human Tagged ORF Clone

CAT＃: RC207577

NR5A1 (Myc-DDK-tagged)-Human nuclear receptor subfamily 5, group A, member 1 (NR5A1)

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_004959" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00

货期*
现货
规格
    • 10 ug
Cited in 10 publications.

Product images

经常一起买 (5)
PS100001
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TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

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【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA806157
NR5A1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)
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【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AD4BP; ELP; FTZ1; FTZF1; hSF-1; POF7; SF-1; SF1; SPGF8; SRXX4; SRXY3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207577 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTATTCGTACGACGAGGACCTGGACGAGCTGTGCCCCGTGTGCGGGGACAAGGTGTCCGGCTACC
ACTACGGACTGCTCACGTGTGAGAGCTGCAAGGGCTTCTTCAAGCGCACGGTGCAGAACAACAAGCACTA
CACGTGCACCGAGAGCCAGAGCTGCAAGATCGACAAGACGCAGCGCAAGCGCTGTCCCTTCTGCCGCTTC
CAGAAATGCCTGACGGTGGGGATGCGCCTGGAAGCCGTGCGCGCTGACCGTATGAGGGGTGGCCGGAACA
AGTTTGGGCCGATGTACAAGCGGGACCGGGCCCTGAAACAGCAGAAGAAGGCACAGATTCGGGCCAATGG
CTTCAAGCTGGAGACAGGGCCCCCGATGGGGGTGCCCCCGCCGCCCCCTCCCGCACCGGACTACGTGCTG
CCTCCCAGCCTGCATGGGCCTGAGCCCAAGGGCCTGGCCGCCGGTCCACCTGCTGGGCCACTGGGCGACT
TTGGGGCCCCAGCACTGCCCATGGCCGTGCCCGGTGCCCACGGGCCACTGGCTGGCTACCTCTACCCTGC
CTTTCCTGGCCGTGCCATCAAGTCTGAGTACCCGGAGCCTTATGCCAGCCCCCCACAGCCTGGGCTGCCG
TACGGCTACCCAGAGCCCTTCTCTGGAGGGCCCAACGTGCCTGAGCTCATCCTGCAGCTGCTGCAGCTGG
AGCCGGATGAGGACCAGGTGCGGGCCCGCATCTTGGGCTGCCTGCAGGAGCCCACCAAAAGCCGCCCCGA
CCAGCCGGCGGCCTTCGGCCTCCTGTGCAGAATGGCCGACCAGACCTTCATCTCCATCGTGGACTGGGCA
CGCAGGTGCATGGTCTTCAAGGAGCTGGAGGTGGCCGACCAGATGACGCTGCTGCAGAACTGCTGGAGCG
AGCTGCTGGTGTTCGACCACATCTACCGCCAGGTCCAGCACGGCAAGGAGGGCAGCATCCTGCTGGTCAC
CGGGCAGGAGGTGGAGCTGACCACAGTGGCCACCCAGGCGGGCTCGCTGCTGCACAGCCTGGTGTTGCGG
GCGCAGGAGCTGGTGCTGCAGCTGCTTGCGCTGCAGCTGGACCGGCAGGAGTTTGTCTGCCTCAAGTTCA
TCATCCTCTTCAGCCTGGATTTGAAGTTCCTGAATAACCACATCCTGGTGAAAGACGCTCAGGAGAAGGC
CAACGCCGCCCTGCTTGACTACACCCTGTGCCACTACCCGCACTGCGGGGACAAATTCCAGCAGCTGCTG
CTGTGCCTGGTGGAGGTGCGGGCCCTGAGCATGCAGGCCAAGGAGTACCTGTACCACAAGCACCTGGGCA
ACGAGATGCCCCGCAACAACCTGCTCATCGAAATGCTGCAAGCCAAGCAGACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RC207577 protein sequence
Red=Cloning site Green=Tags(s)

MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKTQRKRCPFCRF
QKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKLETGPPMGVPPPPPPAPDYVL
PPSLHGPEPKGLAAGPPAGPLGDFGAPALPMAVPGAHGPLAGYLYPAFPGRAIKSEYPEPYASPPQPGLP
YGYPEPFSGGPNVPELILQLLQLEPDEDQVRARILGCLQEPTKSRPDQPAAFGLLCRMADQTFISIVDWA
RRCMVFKELEVADQMTLLQNCWSELLVFDHIYRQVQHGKEGSILLVTGQEVELTTVATQAGSLLHSLVLR
AQELVLQLLALQLDRQEFVCLKFIILFSLDLKFLNNHILVKDAQEKANAALLDYTLCHYPHCGDKFQQLL
LCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004959
ORF Size 1383 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004959.5
RefSeq Size 3095 bp
RefSeq ORF 1386 bp
Locus ID 2516
UniProt ID Q13285
Protein Families Druggable Genome, Transcription Factors
MW 51.6 kDa
Gene Summary The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (10)

The use of this cDNA Clones has been cited in the following citations:
FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture ,Li, YH;Chen, TM;Huang, BM;Yang, SH;Wu, CC;Lin, YM;Chuang, JI;Tsai, SJ;Sun, HS;, Biol. Reprod. ,PubMed ID 32886743 [Steroidogenic Factor 1]
Novel NR5A1 Pathogenic Variants Cause Phenotypic Heterogeneity in 46,XY Disorders of Sex Development ,Sudhakar, DVS;Jaishankar, S;Regur, P;Kumar, U;Singh, R;Kabilan, U;Namduri, S;Dhyani, J;Gupta, NJ;Chakravarthy, B;Vaman, K;Shabir, I;Khadgawat, R;Deenadayal, M;Chaitanya A, D;Dada, R;Sharma, Y;Anand, A;Thangaraj, K;, Sex Dev ,PubMed ID 32008008 [Steroidogenic Factor 1]
Analysis of NR5A1 in 142 patients with premature ovarian insufficiency, diminished ovarian reserve, or unexplained infertility ,Jaillard, S;Sreenivasan, R;Beaumont, M;Robevska, G;Dubourg, C;Knarston, IM;Akloul, L;van den Bergen, J;Odent, S;Croft, B;Jouve, G;Grover, SR;Duros, S;Pimentel, C;Belaud-Rotureau, MA;Ayers, KL;Ravel, C;Tucker, EJ;Sinclair, AH;, Maturitas ,PubMed ID 31787151 [Steroidogenic Factor 1]
Analysis of variants in GATA4 and FOG2 / ZFPM2 demonstrates benign contribution to 46,XY disorders of sex development ,null, Molecular Genetics & Genomic Medicine ,PubMed ID 31962012 [Steroidogenic Factor 1]
NR5A1 gene variants repress the ovarian-specific WNT signalling pathway in 46,XX Disorders of sex development patients ,Knarston, IM;Robevska, G;van den Bergen, JA;Eggers, S;Croft, B;Yates, J;Hersmus, R;Looijenga, LHJ;Cameron, FJ;Monhike, K;Ayers, KL;Sinclair, AH;, Hum. Mutat. ,PubMed ID 30350900 [Steroidogenic Factor 1]
Functional characterisation of novel NR5A1 variants reveals multiple complex roles in Disorders of Sex Development ,Robevska, G;van den Bergen, JA;Ohnesorg, T;Eggers, S;Hanna, C;Hersmus, R;Thompson, EM;Baxendale, A;Verge, CF;Lafferty, AR;Marzuki, NS;Santosa, A;Listyasari, NA;Riedl, S;Warne, G;Looijenga, L;Faradz, S;Ayers, KL;Sinclair, AH;, Hum. Mutat. ,PubMed ID 29027299 [Steroidogenic Factor 1]
Novel Heterozygous Mutations of NR5A1 and Their Functional Characteristics in Patients with 46,XY Disorders of Sex Development without Adrenal Insufficiency. ,null, Hormone research in paediatrics ,PubMed ID 26139438 [Steroidogenic Factor 1]
Whole exome sequencing combined with linkage analysis identifies a novel 3?bp deletion in NR5A1 ,Eggers, S;Smith, KR;Bahlo, M;Looijenga, LH;Drop, SL;Juniarto, ZA;Harley, VR;Koopman, P;Faradz, SM;Sinclair, AH;, Eur. J. Hum. Genet. ,PubMed ID 25099250 [Steroidogenic Factor 1]
cAMP-stimulated transcription of DGK{theta} requires steroidogenic factor 1 and sterol regulatory element binding protein 1 ,Kai Cai and Marion B. Sewer, J. Lipid Res., Aug 2013; 54: 2121 - 2132. ,PubMed ID 23610160 [Steroidogenic Factor 1]
Acid Ceramidase (ASAH1) Represses Steroidogenic Factor 1-Dependent Gene Transcription in H295R Human Adrenocortical Cells by Binding to the Receptor ,Natasha C. Lucki, Donghui Li, Sibali Bandyopadhyay, Elaine Wang, Alfred H. Merrill, and Marion B. Sewer, Mol. Cell. Biol., Nov 2012; 32: 4419 - 4431. [Steroidogenic Factor 1]

Other Versions

SKU Description Size Price
RC207577L1 Lenti ORF clone of Human nuclear receptor subfamily 5, group A, member 1 (NR5A1), Myc-DDK-tagged
    • 10 ug

CNY 5,890.00

RC207577L2 Lenti ORF clone of Human nuclear receptor subfamily 5, group A, member 1 (NR5A1), mGFP tagged
    • 10 ug

CNY 5,890.00

RC207577L3 Lenti ORF clone of Human nuclear receptor subfamily 5, group A, member 1 (NR5A1), Myc-DDK-tagged
    • 10 ug

CNY 5,890.00

RC207577L4 Lenti ORF clone of Human nuclear receptor subfamily 5, group A, member 1 (NR5A1), mGFP tagged
    • 10 ug

CNY 5,890.00

RG207577 NR5A1 (tGFP-tagged) - Human nuclear receptor subfamily 5, group A, member 1 (NR5A1)
    • 10 ug

CNY 4,370.00

SC303555 NR5A1 (untagged)-Human nuclear receptor subfamily 5, group A, member 1 (NR5A1)
    • 10 ug

CNY 3,656.00
CNY 7,220.00

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