PIGU (NM_080476) Human Tagged ORF Clone

CAT#: RC207146

  • TrueORF®

PIGU (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class U (PIGU)

ORF Plasmid: DDK tGFP



  "NM_080476" in other vectors (4)

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CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PIGU Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CDC91L1; GAB1; GPIBD21; NEDBSS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207146 representing NM_080476
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTCCCTTGGTCCTGGTGCTGGTGGTGGCTGTGACAGTGCGGGCGGCCTTGTTCCGCTCCAGTC
TGGCCGAGTTCATTTCCGAGCGGGTGGAGGTGGTGTCCCCACTGAGCTCTTGGAAGAGAGTGGTTGAAGG
CCTTTCACTGTTGGACTTGGGAGTATCTCCGTATTCTGGAGCAGTATTTCATGAAACTCCATTAATAATA
TACCTCTTTCATTTCCTAATTGACTATGCTGAATTGGTGTTTATGATAACTGATGCACTCACTGCTATTG
CCCTGTATTTTGCAATCCAGGACTTCAATAAAGTTGTGTTTAAAAAGCAGAAACTCCTCCTAGAACTGGA
CCAGTATGCCCCAGATGTGGCCGAACTCATCCGGACCCCTATGGAAATGCGTTACATCCCTTTGAAAGTG
GCCCTGTTCTATCTCTTAAATCCTTACACGATTTTGTCTTGTGTTGCCAAGTCTACCTGTGCCATCAACA
ACACCCTCATTGCTTTCTTCATTTTGACTACGATAAAAGGCAGTGCTTTCCTCAGTGCTATTTTTCTTGC
CTTAGCGACATACCAGTCTCTGTACCCACTCACCTTGTTTGTCCCAGGACTCCTCTATCTCCTCCAGCGG
CAGTACATACCTGTGAAAATGAAGAGCAAAGCCTTCTGGATCTTTTCTTGGGAGTATGCCATGATGTATG
TGGGAAGCCTAGTGGTAATCATTTGCCTCTCCTTCTTCCTTCTCAGCTCTTGGGATTTCATCCCCGCAGT
CTATGGCTTTATACTTTCTGTTCCAGATCTCACTCCAAACATTGGTCTTTTCTGGTACTTCTTTGCAGAG
ATGTTTGAGCACTTCAGCCTCTTCTTTGTATGTGTGTTTCAGATCAACGTCTTCTTCTACACCATCCCCT
TAGCCATAAAGCTAAAGGAGCACCCCATCTTCTTCATGTTTATCCAGATCGCTGTCATCGCCATCTTTAA
GTCCTACCCGACAGTGGGGGACGTGGCGCTCTACATGGCCTTCTTCCCCGTGTGGAACCATCTCTACAGA
TTCCTGAGAAACATCTTTGTCCTCACCTGCATCATCATCGTCTGTTCCCTGCTCTTCCCTGTCCTGTGGC
ACCTCTGGATTTATGCAGGAAGTGCCAACTCTAATTTCTTTTATGCCATCACACTGACCTTCAACGTTGG
GCAGATCCTGCTCATCTCTGATTACTTCTATGCCTTCCTGCGGCGGGAGTACTACCTCACACATGGCCTC
TACTTGACCGCCAAGGATGGCACAGAGGCCATGCTCGTGCTCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207146 representing NM_080476
Red=Cloning site Green=Tags(s)

MAAPLVLVLVVAVTVRAALFRSSLAEFISERVEVVSPLSSWKRVVEGLSLLDLGVSPYSGAVFHETPLII
YLFHFLIDYAELVFMITDALTAIALYFAIQDFNKVVFKKQKLLLELDQYAPDVAELIRTPMEMRYIPLKV
ALFYLLNPYTILSCVAKSTCAINNTLIAFFILTTIKGSAFLSAIFLALATYQSLYPLTLFVPGLLYLLQR
QYIPVKMKSKAFWIFSWEYAMMYVGSLVVIICLSFFLLSSWDFIPAVYGFILSVPDLTPNIGLFWYFFAE
MFEHFSLFFVCVFQINVFFYTIPLAIKLKEHPIFFMFIQIAVIAIFKSYPTVGDVALYMAFFPVWNHLYR
FLRNIFVLTCIIIVCSLLFPVLWHLWIYAGSANSNFFYAITLTFNVGQILLISDYFYAFLRREYYLTHGL
YLTAKDGTEAMLVLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080476
ORF Size 1305 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080476.5
RefSeq Size 1833 bp
RefSeq ORF 1308 bp
Locus ID 128869
UniProt ID Q9H490
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
MW 49.9 kDa
Gene Summary The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Cdc91, a predicted integral membrane protein that may function in cell division control. The protein encoded by this gene is the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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