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CNOT1 (NM_206999) Human Tagged Lenti ORF Clone
CAT＃: RC206903L4
- LentiORF®
Lenti-ORF clone of CNOT1 (mGFP-tagged)-Human CCR4-NOT transcription complex, subunit 1 (CNOT1), transcript variant 2
"NM_206999" in other vectors (4)
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CNY 7,600.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | AD-005; CDC39; HPE12; NOT1; NOT1H |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC206903).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_206999 |
| ORF Size | 4653 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_206999.1 |
| RefSeq Size | 5019 bp |
| RefSeq ORF | 4656 bp |
| Locus ID | 23019 |
| UniProt ID | A5YKK6 |
| Protein Pathways | RNA degradation |
| MW | 173.6 kDa |
| Gene Summary | Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206903 | CNOT1 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 1 (CNOT1), transcript variant 2 |
CNY 16,976.00 |
|
| RC206903L3 | Lenti-ORF clone of CNOT1 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 1 (CNOT1), transcript variant 2 |
CNY 7,600.00 |
|
| RG206903 | CNOT1 (tGFP-tagged) - Human CCR4-NOT transcription complex, subunit 1 (CNOT1), transcript variant 2 |
CNY 18,576.00 |
|
| SC126464 | CNOT1 (untagged)-Human CCR4-NOT transcription complex, subunit 1 (CNOT1), transcript variant 2 |
CNY 10,450.00 |
