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HHIP (NM_022475) Human Tagged ORF Clone
CAT＃: RC206868
- TrueORF®
HHIP (Myc-DDK-tagged)-Human hedgehog interacting protein (HHIP)
ORF Plasmid: tGFP
"NM_022475" in other vectors (6)
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CNY 5,128.00
CNY 5,800.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HIP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206868 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAGATGCTCTCCTTTAAGCTGCTGCTGCTGGCCGTGGCTCTGGGCTTCTTTGAAGGAGATGCTA AGTTTGGGGAAAGAAACGAAGGGAGCGGAGCAAGGAGGAGAAGGTGCCTGAATGGGAACCCCCCGAAGCG CCTGAAAAGGAGAGACAGGAGGATGATGTCCCAGCTGGAGCTGCTGAGTGGGGGAGAGATGCTGTGCGGT GGCTTCTACCCTCGGCTGTCCTGCTGCCTGCGGAGTGACAGCCCGGGGCTAGGGCGCCTGGAGAATAAGA TATTTTCTGTTACCAACAACACAGAATGTGGGAAGTTACTGGAGGAAATCAAATGTGCACTTTGCTCTCC ACATTCTCAAAGCCTGTTCCACTCACCTGAGAGAGAAGTCTTGGAAAGAGACCTAGTACTTCCTCTGCTC TGCAAAGACTATTGCAAAGAATTCTTTTACACTTGCCGAGGCCATATTCCAGGTTTCCTTCAAACAACTG CGGATGAGTTTTGCTTTTACTATGCAAGAAAAGATGGTGGGTTGTGCTTTCCAGATTTTCCAAGAAAACA AGTCAGAGGACCAGCATCTAACTACTTGGACCAGATGGAAGAATATGACAAAGTGGAAGAGATCAGCAGA AAGCACAAACACAACTGCTTCTGTATTCAGGAGGTTGTGAGTGGGCTGCGGCAGCCCGTTGGTGCCCTGC ATAGTGGGGATGGCTCGCAACGTCTCTTCATTCTGGAAAAAGAAGGTTATGTGAAGATACTTACCCCTGA AGGAGAAATTTTCAAGGAGCCTTATTTGGACATTCACAAACTTGTTCAAAGTGGAATAAAGGGAGGAGAT GAAAGAGGACTGCTAAGCCTCGCATTCCATCCCAATTACAAGAAAAATGGAAAGTTGTATGTGTCCTATA CCACCAACCAAGAACGGTGGGCTATCGGGCCTCATGACCACATTCTTAGGGTTGTGGAATACACAGTATC CAGAAAAAATCCACACCAAGTTGATTTGAGAACAGCCAGAGTCTTTCTTGAAGTTGCAGAACTCCACAGA AAGCATCTGGGAGGACAACTGCTCTTTGGCCCTGACGGCTTTTTGTACATCATTCTTGGTGATGGGATGA TTACACTGGATGATATGGAAGAAATGGATGGGTTAAGTGATTTCACAGGCTCAGTGCTACGGCTGGATGT GGACACAGACATGTGCAACGTGCCTTATTCCATACCAAGGAGCAACCCACACTTCAACAGCACCAACCAG CCCCCCGAAGTGTTTGCTCATGGGCTCCACGATCCAGGCAGATGTGCTGTGGATAGACATCCCACTGATA TAAACATCAATTTAACGATACTGTGTTCAGACTCCAATGGAAAAAACAGATCATCAGCCAGAATTCTACA GATAATAAAGGGGAAAGATTATGAAAGTGAGCCATCACTTTTAGAATTCAAGCCATTCAGTAATGGTCCT TTGGTTGGTGGATTTGTATACCGGGGCTGCCAGTCAGAAAGATTGTATGGAAGCTACGTGTTTGGAGATC GTAATGGGAATTTCCTAACTCTCCAGCAAAGTCCTGTGACAAAGCAGTGGCAAGAAAAACCACTCTGTCT CGGCACTAGTGGGTCCTGTAGAGGCTACTTTTCCGGTCACATCTTGGGATTTGGAGAAGATGAACTAGGT GAAGTTTACATTTTATCAAGCAGTAAAAGTATGACCCAGACTCACAATGGAAAACTCTACAAAATTGTAG ATCCCAAAAGACCTTTAATGCCTGAGGAATGCAGAGCCACGGTACAACCTGCACAGACACTGACTTCAGA GTGCTCCAGGCTCTGTCGAAACGGCTACTGCACCCCCACGGGAAAGTGCTGCTGCAGTCCAGGCTGGGAG GGGGACTTCTGCAGAACTGCAAAATGTGAGCCAGCATGTCGTCATGGAGGTGTCTGTGTTAGACCGAACA AGTGCCTCTGTAAAAAAGGATATCTTGGTCCTCAATGTGAACAAGTGGACAGAAACATCCGCAGAGTGAC CAGGGCAGGTATTCTTGATCAGATCATTGACATGACATCTTACTTGCTGGATCTAACAAGTTACATTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206868 protein sequence
Red=Cloning site Green=Tags(s) MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCG GFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREVLERDLVLPLL CKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISR KHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIKGGD ERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHR KHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQ PPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGP LVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGTSGSCRGYFSGHILGFGEDELG EVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRATVQPAQTLTSECSRLCRNGYCTPTGKCCCSPGWE GDFCRTAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNIRRVTRAGILDQIIDMTSYLLDLTSYIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022475 |
| ORF Size | 2100 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022475.3 |
| RefSeq Size | 3555 bp |
| RefSeq ORF | 2103 bp |
| Locus ID | 64399 |
| UniProt ID | Q96QV1 |
| Domains | EGF |
| Protein Families | Secreted Protein |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
| MW | 78.9 kDa |
| Gene Summary | This gene encodes a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.[provided by RefSeq, Feb 2011] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Downregulation of hedgehog-interacting protein (HHIP) contributes to hexavalent chromium-induced malignant transformation of human bronchial epithelial cells
,Li, P;Zhang, X;Murphy, AJ;Costa, M;Zhao, X;Sun, H;,
Carcinogenesis
,PubMed ID 32710611
[HHIP]
|
|
Hypoxia Triggers Hedgehog-Mediated Tumor–Stromal Interactions in Pancreatic Cancer
,Taly R. Spivak-Kroizman, Galen Hostetter, Richard Posner, Meraj Aziz, Chengcheng Hu, Michael J. Demeure, Daniel Von Hoff, Sunil R. Hingorani, Timothy B. Palculict, Julie Izzo, Galina M. Kiriakova, Mena Abdelmelek, Geoffrey Bartholomeusz, Brian P. James, and Garth Powis,
Cancer Res., Jun 2013; 73: 3235 - 3247.
,PubMed ID 23633488
[HHIP]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206868L1 | Lenti ORF clone of Human hedgehog interacting protein (HHIP), Myc-DDK-tagged |
CNY 7,528.00 |
|
| RC206868L2 | Lenti ORF clone of Human hedgehog interacting protein (HHIP), mGFP tagged |
CNY 7,700.00 |
|
| RC206868L3 | Lenti ORF clone of Human hedgehog interacting protein (HHIP), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC206868L4 | Lenti ORF clone of Human hedgehog interacting protein (HHIP), mGFP tagged |
CNY 7,700.00 |
|
| RG206868 | HHIP (tGFP-tagged) - Human hedgehog interacting protein (HHIP) |
CNY 6,370.00 |
|
| SC120676 | HHIP (untagged)-Human hedgehog interacting protein (HHIP) |
CNY 2,950.00 |
