PDCL2 (NM_152401) Human Tagged ORF Clone

CAT#: RC206703

  • TrueORF®

PDCL2 (Myc-DDK-tagged)-Human phosducin-like 2 (PDCL2)

ORF Plasmid: DDK tGFP



  "NM_152401" in other vectors (4)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


PDCL2 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms GCPHLP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206703 representing NM_152401
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGATCCCAATGAAGATACAGAATGGAATGACATTTTAAGAGATTTCGGCATTCTTCCTCCTAAAG
AAGAGTCAAAAGATGAAATTGAAGAAATGGTTTTACGTTTACAGAAAGAAGCAATGGTGAAACCATTTGA
AAAGATGACTCTTGCACAGCTAAAGGAAGCTGAAGATGAATTTGATGAAGAAGATATGCAGGCTGTTGAA
ACATATAGAAAGAAGCGGTTACAGGAATGGAAAGCTCTTAAGAAAAAACAAAAATTTGGAGAATTAAGAG
AAATTTCTGGAAATCAGTATGTGAATGAAGTCACAAATGCAGAAGAAGATGTGTGGGTTATAATTCATCT
ATACAGATCAAGCATCCCAATGTGTTTGTTGGTTAACCAGCATCTTAGTCTTCTAGCAAGAAAGTTTCCA
GAAACTAAATTTGTTAAAGCCATCGTGAATAGCTGTATTCAACACTACCATGACAATTGTTTACCAACAA
TTTTTGTGTATAAAAATGGTCAGATAGAAGCCAAATTCATTGGAATTATAGAATGTGGAGGGATAAATCT
CAAGCTGGAAGAACTTGAATGGAAGCTAGCAGAAGTTGGAGCAATACAGACTGATTTGGAAGAAAACCCC
AGAAAAGACATGGTAGATATGATGGTATCTTCAATTAGAAACACTTCTATTCATGATGACAGTGATAGCT
CCAACAGTGATAATGATACCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206703 representing NM_152401
Red=Cloning site Green=Tags(s)

MQDPNEDTEWNDILRDFGILPPKEESKDEIEEMVLRLQKEAMVKPFEKMTLAQLKEAEDEFDEEDMQAVE
TYRKKRLQEWKALKKKQKFGELREISGNQYVNEVTNAEEDVWVIIHLYRSSIPMCLLVNQHLSLLARKFP
ETKFVKAIVNSCIQHYHDNCLPTIFVYKNGQIEAKFIGIIECGGINLKLEELEWKLAEVGAIQTDLEENP
RKDMVDMMVSSIRNTSIHDDSDSSNSDNDTK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_152401
ORF Size 723 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_152401.3
RefSeq Size 873 bp
RefSeq ORF 726 bp
Locus ID 132954
UniProt ID Q8N4E4
MW 28.5 kDa
Gene Summary This gene encodes a member of the phosducin-like protein family and is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin. Members of the phosducin-like protein family have been shown to bind to the beta-gamma subunits of G proteins. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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