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P4HA1 (NM_001017962) Human Tagged ORF Clone
CAT＃: RC206055
P4HA1 (Myc-DDK-tagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 2
ORF Plasmid: tGFP
"NM_001017962" in other vectors (6)
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CNY 3,976.00
CNY 3,990.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | P4HA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206055 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTTACTT CAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTGAAAGATTATATTAA GGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAAGTTAGATCGGCTAACTAGTACAGCG ACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGTAAATGCATTCAAATTAATGAAACGTCTGAATACTG AGTGGAGTGAGTTGGAGAATCTGGTCCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCA GAGACAGTACTTTCCTAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACC TACAATTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACAAATCTTTTCTAACGG CTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCATACGGAACTGTGGAT GGAACAAGCCCTAAGGCAACTGGATGAAGGCGAGATTTCTACCATAGATAAAGTCTCTGTTCTAGATTAT TTGAGCTATGCGGTATATCAGCAGGGAGACCTGGATAAGGCACTTTTGCTCACAAAGAAGCTTCTTGAAC TAGATCCTGAACATCAGAGAGCTAATGGTAACTTAAAATATTTTGAGTATATAATGGCTAAAGAAAAAGA TGTCAATAAGTCTGCTTCAGATGACCAATCTGATCAGAAAACTACACCAAAGAAAAAAGGGGTTGCTGTG GATTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACCCCTCGGA GACAGAAAAAACTCTTTTGCCGCTACCATGATGGAAACCGTAATCCTAAATTTATTCTGGCTCCAGCTAA ACAGGAGGATGAATGGGACAAGCCTCGTATTATTCGCTTCCATGATATTATTTCTGATGCAGAAATTGAA ATCGTCAAAGACCTAGCAAAACCAAGGCTGAGGCGAGCCACCATTTCAAACCCAATAACAGGAGACTTGG AGACGGTACATTACAGAATTAGCAAAAGTGCCTGGCTCTCTGGCTATGAAAATCCTGTGGTGTCTCGAAT TAATATGAGAATACAAGATCTAACAGGACTAGATGTTTCCACAGCAGAGGAATTACAGGTAGCAAATTAT GGAGTTGGAGGACAGTATGAACCCCATTTTGACTTTGCACGGAAAGATGAGCCAGATGCTTTCAAAGAGC TGGGGACAGGAAATAGAATTGCTACATGGCTGTTTTATATGAGTGATGTGTCTGCAGGAGGAGCCACTGT TTTTCCTGAAGTTGGAGCTAGTGTTTGGCCCAAAAAAGGAACTGCTGTTTTCTGGTATAATCTGTTTGCC AGTGGAGAAGGAGATTATAGTACACGGCATGCAGCCTGTCCAGTGCTAGTTGGCAACAAATGGGTATCCA ATAAATGGCTCCATGAACGTGGACAAGAATTTCGAAGACCTTGTACGTTGTCAGAATTGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206055 protein sequence
Red=Cloning site Green=Tags(s) MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTA TKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDT YNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDY LSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAV DYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIE IVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANY GVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFA SGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001017962 |
| ORF Size | 1602 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001017962.2, NP_001017962.1 |
| RefSeq Size | 2860 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 5033 |
| UniProt ID | P13674 |
| Protein Families | Druggable Genome, P450 |
| Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
| MW | 61 kDa |
| Gene Summary | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206055L1 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206055L2 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC206055L3 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206055L4 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG206055 | P4HA1 (tGFP-tagged) - Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 2 |
CNY 4,370.00 |
|
| SC302089 | P4HA1 (untagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 2 |
CNY 7,220.00 |
