DPPA2 (NM_138815) Human Tagged ORF Clone

CAT#: RC205441

  • TrueORF®

DPPA2 (Myc-DDK-tagged)-Human developmental pluripotency associated 2 (DPPA2)

ORF Plasmid: DDK tGFP



  "NM_138815" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


DPPA2 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CT100; ECAT15-2; PESCRG1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205441 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGATGCAAATTTGGATAGCAGCAAGAAGAATTTCTTGGAGGGGGAAGTAGATGATGAGGAAAGTG
TGATTTTGACACTGGTGCCAGTTAAAGATGACGCAAATATGGAACAAATGGAACCAAGCGTTTCTTCAAC
TTCTGATGTCAAACTGGAGAAGCCTAAGAAATACAATCCAGGTCATCTACTTCAAACAAATGAGCAATTT
ACAGCTCCACAAAAAGCTAGATGCAAAATACCAGCCCTTCCCTTGCCGACCATTTTGCCTCCCATTAATA
AGGTGTGTCGGGACACTTTGCGGGACTGGTGTCAACAACTCGGTTTGAGTACTAATGGCAAGAAAATCGA
AGTTTATCTGAGGCTTCATAGGCATGCTTACCCTGAACAACGGCAAGATATGCCTGAAATGTCACAAGAG
ACCAGATTACAGCGATGTTCGAGGAAACGCAAGGCAGTGACCAAGAGAGCAAGGCTTCAGAGAAGTTATG
AGATGAATGAGAGAGCAGAAGAGACCAATACAGTTGAAGTGATAACTTCAGCACCGGGAGCCATGTTGGC
ATCATGGGCAAGAATTGCTGCAAGAGCTGTTCAGCCTAAGGCTTTGAATTCATGTTCCATTCCTGTTTCT
GTTGAGGCCTTTTTGATGCAAGCCTCTGGCGTCAGGTGGTGTGTGGTCCATGGCAGACTTCTCTCGGCAG
ACACAAAGGGTTGGGTACGCCTGCAGTTTCATGCAGGTCAGGCCTGGGTGCCTACCACTCACAGGAGGAT
GATTTCTCTCTTCTTGTTACCTGCCTGCATTTTCCCATCCCCAGGCATAGAAGATAATATGTTATGCCCC
GACTGTGCTAAGAGGAATAAGAAGATGATGAAAAGATTAATGACAGTAGAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205441 protein sequence
Red=Cloning site Green=Tags(s)

MSDANLDSSKKNFLEGEVDDEESVILTLVPVKDDANMEQMEPSVSSTSDVKLEKPKKYNPGHLLQTNEQF
TAPQKARCKIPALPLPTILPPINKVCRDTLRDWCQQLGLSTNGKKIEVYLRLHRHAYPEQRQDMPEMSQE
TRLQRCSRKRKAVTKRARLQRSYEMNERAEETNTVEVITSAPGAMLASWARIAARAVQPKALNSCSIPVS
VEAFLMQASGVRWCVVHGRLLSADTKGWVRLQFHAGQAWVPTTHRRMISLFLLPACIFPSPGIEDNMLCP
DCAKRNKKMMKRLMTVEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_138815
ORF Size 894 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_138815.4
RefSeq Size 1393 bp
RefSeq ORF 897 bp
Locus ID 151871
UniProt ID Q7Z7J5
Protein Families Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency
MW 33.8 kDa
Gene Summary Binds to target gene promoters, including NKX2-5 and SYCE1, but not GATA4, and may be involved in the maintenance of the active epigenetic status of these genes.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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